Analysis of Differential Expression Genes in Leaves of Leaf Color Mutant of Chinese Orchid

doi:10.16420/j.issn.0513-353x.2021-0950

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 371-381.doi: 10.16420/j.issn.0513-353x.2021-0950

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Analysis of Differential Expression Genes in Leaves of Leaf Color Mutant of Chinese Orchid

JIANG Yu, TU Xunliang, HE Junrong^*()

Institute of Horticulture，Sichuan Academy of Agricultural Sciences，Chengdu 610000，China

Received:2022-09-26 Revised:2022-12-05 Online:2023-02-25 Published:2023-03-06
Contact: *（E-mail：junrh@126.com）

Abstract

Abstract:

The Chinses orchid‘Longchangsu’and its leaf color mutant‘Yeyi Longchangsu’are excellent materials to study the formation mechanism of the leaf color of orchid. Differentially expressed genes（DEGs）in the leaves of‘Longchangsu’and‘Yeyi Longchangsu’were identifed by next-generation sequencing using Illumina Hiseq2500. Through the analysis of different expression genes，it was found that the genes related to pigment synthesis and chloroplast development might be the cause of the differences between‘Longchangsu’and‘Yeyi Longchangsu’.

Key words: Chinese orchid, leaf, leaf color, transcriptome

CLC Number:

S682.31

JIANG Yu, TU Xunliang, HE Junrong. Analysis of Differential Expression Genes in Leaves of Leaf Color Mutant of Chinese Orchid[J]. Acta Horticulturae Sinica, 2023, 50(2): 371-381.

Figures/Tables 10

References 35

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基因名称 Gene name	引物序列（5′-3′） Primers sequence
c19370_g1	F：CGCTGTCCAGCTCCCC；R：CGCTCAATCGCCCAAA
c59163_g1	F：TTTGGAAGACGAACAA；R：CTGGAGAAGGAGGTGC
c2728_g1	F：TGGTGCTGGCAAGATG；R：ACGCAGTGCTGGTGAA
c7212_g1	F：TCGTCTTCATCCGCCA；R：CCCAACCATAATCCCC
c63240_g1	F：GGGTGGGTTGGATTAT；R：TTGCCTGGGTAGAGTG
c100428_g1	F：CTACTGAGGCTGGCTT；R：GGATTGTCGCTGGAAA
c 42120_g1	F：AACGATCCAGACCAGG；R：TCTCTCAGCAGTGCCA
c404_g1	F：CTAATAACGACCCAAT；R：GCAAAGAACACACAAA
c42905_g1	F：GGTCTTACCACCATCA；R：GGGAACCTTTTCTCTA
c2922_g1	F：CATCCATTGCTTGTCG；R：ATCATTCTGCTCTGCC
c80392_g1	F：CATTGCGTTCATCGTT；R：CAAGCCCCTCTTTTTT
c32780_g1	F：TTCACTGGTTACTCTC；R：AACTCAATCTTGTTCT
c40734_g1	F：CCTCACGACCTTTCAA；R：CTTTCCCCACTCTCTG
c62684_g1	F：GTTGCTCGGCTGTTCG；R：AGTTGATGGTTTGGGA
c65620_g1	F：TTGTAGCAACCAAGGC；R：ACGGAAAAGAAAATCT
ACTIN-7	F：AATCCCAAGGCAAACAGA；R：CCATACCAGAATCCAG

基因名称 Gene name	引物序列（5′-3′） Primers sequence
c19370_g1	F：CGCTGTCCAGCTCCCC；R：CGCTCAATCGCCCAAA
c59163_g1	F：TTTGGAAGACGAACAA；R：CTGGAGAAGGAGGTGC
c2728_g1	F：TGGTGCTGGCAAGATG；R：ACGCAGTGCTGGTGAA
c7212_g1	F：TCGTCTTCATCCGCCA；R：CCCAACCATAATCCCC
c63240_g1	F：GGGTGGGTTGGATTAT；R：TTGCCTGGGTAGAGTG
c100428_g1	F：CTACTGAGGCTGGCTT；R：GGATTGTCGCTGGAAA
c 42120_g1	F：AACGATCCAGACCAGG；R：TCTCTCAGCAGTGCCA
c404_g1	F：CTAATAACGACCCAAT；R：GCAAAGAACACACAAA
c42905_g1	F：GGTCTTACCACCATCA；R：GGGAACCTTTTCTCTA
c2922_g1	F：CATCCATTGCTTGTCG；R：ATCATTCTGCTCTGCC
c80392_g1	F：CATTGCGTTCATCGTT；R：CAAGCCCCTCTTTTTT
c32780_g1	F：TTCACTGGTTACTCTC；R：AACTCAATCTTGTTCT
c40734_g1	F：CCTCACGACCTTTCAA；R：CTTTCCCCACTCTCTG
c62684_g1	F：GTTGCTCGGCTGTTCG；R：AGTTGATGGTTTGGGA
c65620_g1	F：TTGTAGCAACCAAGGC；R：ACGGAAAAGAAAATCT
ACTIN-7	F：AATCCCAAGGCAAACAGA；R：CCATACCAGAATCCAG

基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
镁—原卟啉Ⅸ甲基转移酶 CHLM-magnesium-protoporphyrin Ⅸ methyltransferase	c19370_g1	2.38	上调Up
谷氨酰-tRNA还原酶 HEMA-glutamyl-tRNA reductase	c59163_g1	2.30	上调Up
尿卟啉原脱羧酶 HEME-uroporphyrinogen decarboxylase	c2728_g1	-1.72	下调Down

基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
镁—原卟啉Ⅸ甲基转移酶 CHLM-magnesium-protoporphyrin Ⅸ methyltransferase	c19370_g1	2.38	上调Up
谷氨酰-tRNA还原酶 HEMA-glutamyl-tRNA reductase	c59163_g1	2.30	上调Up
尿卟啉原脱羧酶 HEME-uroporphyrinogen decarboxylase	c2728_g1	-1.72	下调Down

基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
玉米黄素环氧化酶ABA1-zeaxanthin epoxidase	c7212_g1	-3.78	下调Down
类胡萝卜素裂解双加氧酶7 CCD7-carotenoid cleavage dioxygenase 7	c63240_g1	-2.32	下调Down
脱落酸8′-羟化酶1 ABAH1-ABA8’-hydroxylases 1	c100428_g1	-1.69	下调Down
类胡萝卜素裂解双加氧酶8 CCD8-carotenoid cleavage dioxygenase 8	c11404_g1	3.40	上调Up
脱落酸8′-羟化酶3 ABAH3-ABA8’-hydroxylases 3	c23549_g1	1.36	上调Up

Analysis of Differential Expression Genes in Leaves of Leaf Color Mutant of Chinese Orchid

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基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
编码光系统Ⅰ Coded photosystem Ⅰ
光系统ⅠP700叶绿素a载脂蛋白A1 psaA-photosystem ⅠP700 chlorophyll a apoprotein A1	c80392_g1	-1.61	下调Down
编码光系统Ⅱ Coded photosystem Ⅱ
光系统Ⅱ蛋白H psbH-photosystem Ⅱ protein H	c83746_g1	-1.36	下调Down
光系统Ⅱ蛋白B psbB-photosystem Ⅱ protein B	c74742_g1	-1.93	下调Down
光系统Ⅱ蛋白I psbI-photosystem Ⅱ protein I	c42120_g1	-2.18	下调Down
光系统Ⅱ蛋白I psbD-photosystem Ⅱ protein I	c404_g1	-2.09	下调Down
光系统Ⅱ蛋白C psbC-photosystem Ⅱ protein C	c42905_g1	-2.26	下调Down
光系统Ⅱ蛋白A psbA-photosystem Ⅱ protein A	c2922_g1	-2.61	下调Down

基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
ATP合酶亚组 ATP synthase subgroup	c32780_g1	-2.37	下调Down
	c40734_g1	-1.98	下调Down
	c59833_g1	-1.64	下调Down
	c66335_g1	-1.55	下调Down
还原型烟酰胺腺嘌呤二核苷酸磷酸 NADPH	c3591_g1	-1.79	下调Down
	c28628_g1	-1.55	下调Down
	c102708_g1	-1.40	下调Down
光系统Q蛋白 Photosystem Q protein	c2922_g1	-2.61	下调Down
叶绿素脱辅基蛋白 Chlorophyll decoprotein	c83746_g1	-2.09	下调Down
	c62614_g1	-1.93	下调Down
光系统I组装蛋白Ycf3蛋白 Photosystem I assembly protein	c64551_g1	-1.67	下调Down
叶绿素脱辅基蛋白 Chlorophyll decoprotein	c80392_g1	-1.61	下调Down
光系统2反应中心蛋白H Photosystem 2 reaction center protein H	c110275_g1	-1.36	下调Down
细胞色素b6复合体 Cytochrome b6 complex	c67804_g1	-1.44	下调Down
	c785_g1	-1.37	下调Down
五肽重复蛋白PPR蛋白 Pentatricopeptide repeat proteins	c40226_g1	-2.83	下调Down

基因注释 Gene annotation	基因名称 Gene name	log₂FC（YL/GL）	表达变化 Change in expression
T-box转录因子 T-box transcription factor	c92579_g1	-8.61	下调Down
锌指蛋白 Zinc finger protein	c64437_g1	-8.22	下调Down
受体激酶蛋白LRR Receptor kinase protein-LRR	c14053_g1	-7.32	下调Down
黄酮类 Flavonoids	c100073_g1	-6.86	下调Down
半胱氨酸蛋白酶 Cysteine protease	c39353_g1	-6.17	下调Down
ATP合酶 ATP synthase	c67276_g1	-5.69	下调Down
OsI_26737蛋白 OsI_26737 protein	c77510_g1	-5.61	下调Down
多聚蛋白 Polymerin	c66610_g1	-5.54	下调Down
LRR类受体蛋白激酶FER LRR class receptor protein kinase FER	c46737_g1	-5.52	下调Down
细胞分裂素 Cytokinin	c107218_g1	-5.43	下调Down
P450细胞色素 P450 cytochrome	c64814_g1	-5.19	下调Down
左旋门冬酰胺酶 L-Olastinase	c19273_g1	-5.02	下调Down
细胞壁蛋白 Cell wall protein	c108447_g1	-4.93	下调Down
ATP 结合蛋白 ATP-binding protein	c32257_g1	-4.69	下调Down
线粒体蛋白 Mitochondrial protein	c60540_g1	-4.56	下调Down
转运蛋白 Transporter	c27327_g2	-4.52	下调Down
转运蛋白 Transporter	c87_g1	-4.35	下调Down
ABC蛋白G家族 ABC protein G family	c84960_g1	-3.69	下调Down
磷酸果糖激酶 Phosphofructokinase	c91817_g1	-3.52	下调Down
ABC蛋白G家族 ABC protein G family	c23768_g1	-3.43	下调Down
磷酸盐转运蛋白 Phosphate transporter	c62018_g1	-3.34	下调Down
Cation/H（+）逆向转运蛋白 Cation/H（+）reverse transporter	c24560_g1	-3.29	下调Down
ATP合酶 ATP synthase	c5340_g1	-3.13	下调Down
无机磷酸盐转运蛋白 Inorganic phosphate transporter	c108071_g1	-2.93	下调Down
P450细胞色素 P450 cytochrome	c81249_g1	-2.92	下调Down
阳离子转运调控蛋白 Cationic transport regulatory proteins	c67213_g1	-2.74	下调Down
热激蛋白70 Heat shock 70 protein	c100558_g1	-2.73	下调Down
转录因子AtMYC2 Transcription factor AtMYC2	c39553_g1	-2.72	下调Down
核糖核酸酶H蛋白 RNase H protein	c66710_g1	-2.71	下调Down
NADH氧化还原酶J亚组 NADH oxidoreductase J subgroup	c3591_g1	-1.79	下调Down
基因注释 Gene annotation	基因 Gene	log₂FC（YL/GL）	表达变化 Change in expression
植物凝集素 Phytolectins	c41468_g1	11.58	上调Up
脂类转移蛋白 Liposomes	c58987_g1	11.03	上调Up
漆酶 Laccase	c60052_g2	10.82	上调Up
脂肪酶 Lipase	c80711_g1	10.50	上调Up
细胞凋亡蛋白酶 Apoptotic protease	c5460_g1	10.28	上调Up
萌发素类蛋白 Germinin proteins	c96935_g1	10.21	上调Up
β-葡糖苷酶 β-Glucosidase	c98081_g1	10.06	上调Up
氧戊二磷酸盐加氧酶 Oxopentadiphosphate oxygenase	c39362_g1	9.76	上调Up
甘氨酸蛋白 Glycine protein	c1241_g1	9.68	上调Up
微管蛋白 Tubulin	c4157_g1	9.66	上调Up
组蛋白H3 Histone H3	c84326_g1	9.63	上调Up
过氧物酶 Peroxygenase	c1459_g1	9.18	上调Up
赤霉素双加氧酶 Gibberellin dioxygenase	c58885_g1	9.11	上调Up
赤霉素调节蛋白 Gibberellin regulatory protein	c84075_g1	8.90	上调Up
微管蛋白 Tubulin	c101614_g1	8.82	上调Up
高柠檬酸激酶 High citrate kinase	c4090_g1	8.53	上调Up
异丙基苹果酸合酶 Isopropyl malate synthase	c105648_g1	8.48	上调Up
甘露糖结合凝激酶 Mannose binds to thrombinase	c84042_g1	8.39	上调Up
NAC蛋白 NAC protein	c62684_g1	8.24	上调Up
ABC转运蛋白G家族 ABC transporter G family	c99352_g1	7.98	上调Up
伸展蛋白 Stretch protein	c101662_g1	7.78	上调Up
细胞核受体蛋白 Nuclear receptor protein	c41338_g1	7.75	上调Up
阳离子过氧化物酶 Cationic peroxidase	c29289_g1	7.59	上调Up
Myb家族蛋白 Myb family protein	c65620_g1	7.52	上调Up
细胞周期蛋白B1 Cyclin B1	c5863_g1	7.51	上调Up
跨膜蛋白45B Transmembrane protein 45B	c61378_g1	7.48	上调Up
伸展蛋白 Stretch protein	c83873_g1	7.30	上调Up

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