Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (8): 1650-1662.doi: 10.16420/j.issn.0513-353x.2021-0518

• Research Papers • Previous Articles     Next Articles

Identification of CslD Gene Family in Pineapple and Functional Analysis of AcoCslD2a

LIU Chaoyang(), LIAO Zhichan, LU Xinxin, HE Yehua   

  1. Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China,Ministry of Agriculture and Rural Affairs,College of Horticulture,South China Agricultural University,Guangzhou 510642,China
  • Received:2022-02-22 Revised:2022-05-09 Online:2022-08-25 Published:2022-09-05


In this study,eight CslD genes were characterized in pineapple genome. Chromosomal mapping showed that they were distributed on seven chromosomes and one scaffold. Three to five exons were identified in these genes. Three subgroups were divided according to the phylogenetic analysis,and the number of Group Ⅲ was significantly increased in pineapple. The potential biological functions of the pineapple CslD gene family members were further predicted,basing on the collinearity and gene expression analysis. The full length cDNA sequence of AcoCslD2a was cloned and the subcellular localization analysis indicated that its encoding protein was localized in Golgi apparatus. Two expression vector for AcoCslD2a gene,which were driven by the promoters including ProAtUbq10 and ProAtCslD3,respectively,were constructed and transformed into the Arabidopsis atcsld3 root hair deficient mutant,mediated by Agrobacterium tumefaciens GV3101. The phenotype analysis for the transgenic lines of different expression vectors both indicated that the defect root hair number phenotype of mutant could be recovered to the wild-type phenotype by AcoCslD2a,while the defect root hair length phenotype could be partially recovered.

Key words: pineapple, cellulose synthase-like, CslD, cell wall, root hair

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