Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (7): 1401-1414.doi: 10.16420/j.issn.0513-353x.2021-0499

• Research Papers •     Next Articles

Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress

ZHENG Xiaodong1, XI Xiangli1, LI Yuqi1, SUN Zhijuan2, MA Changqing1, HAN Mingsan3, LI Shaoxuan3, TIAN Yike1, WANG Caihong1,**()   

  1. 1Laboratory of Fruit Tree Genetic Improvement and Biotechnology,College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China
    2College of Life Science,Qingdao Agricultural University,Qingdao, Shandong 266109,China
    3Qingdao Academy of Agricultural Sciences,Qingdao,Shandong 266000,China
  • Received:2022-01-17 Revised:2022-03-29 Online:2022-07-25 Published:2022-07-29
  • Contact: WANG Caihong


In order to explore the effect of brassinosteroids on the growth of apple plants under saline-alkali stress,apple rootstock Malus hupehensis with apomixis characteristics were used as experimental materials. The study investigated the effects of exogenous EBL(brassinosteroids analogs)on ion homeostasis,osmotic regulation,antioxidant system and pH balance of M. hupehensis under saline-alkali stress. The results showed that application of 0.2 mg · L-1 exogenous EBL could significantly enhance the tolerance of M. hupehensis to saline-alkali stress. The main mechanisms were as follows:exogenous EBL could regulate the expression of Na+/K+ transporter genes and enhance the ratio of potassium to sodium to maintain ion homeostasis. Moreover,exogenous EBL could accumulate proline and soluble sugar to modulate osmotic stress,and enhance the activities of POD and CAT to alleviate oxidative damage under saline-alkali stress. Furthermore,exogenous EBL could decrease root pH by improving root secretion of organic acids and enhancing the expression of MhAHA2 and MhAHA8 genes. Exogenous EBL could also cooperate with auxin,gibberellin and dihydrozeatin responding to saline-alkali stress.

Key words: apple, Malus hupehensis, rootstock, saline-alkali stress, brassinosteroids, ion homeostasis, oxidative damage

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