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园艺学报 ›› 2022, Vol. 49 ›› Issue (2): 352-364.doi: 10.16420/j.issn.0513-353x.2020-1013

• 研究论文 • 上一篇    下一篇

向日葵LACS家族鉴定及响应非生物胁迫表达分析

周至铭, 杨佳宝, 张程, 曾令露, 孟晚秋, 孙黎()   

  1. 石河子大学生命科学学院,新疆石河子 832003
  • 收稿日期:2021-07-01 修回日期:2021-12-23 出版日期:2022-02-25 发布日期:2022-02-28
  • 通讯作者: 孙黎 E-mail:sunlishz@126.com
  • 基金资助:
    国家自然科学基金项目(31760064);国家自然科学基金项目(31360052)

Genome-wide Identification and Expression Analyses of Long-chain Acyl-CoA Synthetases Under Abiotic Stresses in Helianthus annuus

ZHOU Zhiming, YANG Jiabao, ZHANG Cheng, ZENG Linglu, MENG Wanqiu, SUN Li()   

  1. College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003,China
  • Received:2021-07-01 Revised:2021-12-23 Online:2022-02-25 Published:2022-02-28
  • Contact: SUN Li E-mail:sunlishz@126.com

摘要:

利用生物信息学技术从向日葵(Helianthus annuus)基因组中鉴定了13个长链酰基辅酶A合成酶(long-chain acyl-CoA synthases,LACSs)家族基因,其分布在向日葵8条染色体上。系统发育树显示,HaLACS家族成员分为6个亚族,且同一亚族成员具有相似的基因结构和蛋白保守基序。基因复制分析发现HaLACS家族扩增的主要因素是片段复制。HaLACS的启动子区域富含逆境响应和植物激素调控相关元件。表达谱分析显示HaLACS在向日葵不同组织中的表达存在差异。比较2个向日葵品种(高/低亚油酸含量)中HaLACS在种子发育不同阶段的表达,HaLACS呈现差异表达。qRT-PCR结果表明,200 mmol · L-1 NaCl处理24 h后,7个HaLACS在茎中的表达量与同期对照相比显著上调;100 μmol · L-1 ABA 处理3 h后,8个HaLACS在根中的表达量显著上调;15% PEG 6000模拟干旱胁迫后,多数HaLACS在不同组织中呈现诱导表达。综上,向日葵LACS基因家族不仅参与了向日葵脂质的合成,而且在应对非生物胁迫时发挥重要作用。

关键词: 向日葵, LACS基因家族, 非生物胁迫, 基因表达

Abstract:

Based on bioinformatics analyses,13 long-chain acyl-CoA synthetases(LACSs)putative genes from LACS gene family were identified from the genome of sunflower(Helianthus annuus)and mapped to eight chromosomes. The phylogenetic analysis showed that HaLACSs clustered into six subgroups and HaLACS proteins within each subgroup share the similar structures and conserved motifs. The main factors for HaLACS family amplification in sunflower were segmental duplication. Bioinformatic analysis of HaLACS genes promoter regions showed multiple cis-elements related to environmental stresses and plant hormone response. Tissue expression profile analysis showed that the HaLACS genes exhibited differential expression profiles in various tissues. The comparison of gene expression profiles between two sunflower cultivars(high/low linoleic acid)found that HaLACSs showed differential expression at different seed development stages. The qRT-PCR analysis showed that seven HaLACS up-regulated significantly in sunflower stem treated with 200 mmol · L-1 NaCl for 24 h compared with control. After three hours treatment with 100 μmol · L-1 ABA,eight HaLACS synchronized especially in roots,and most HaLACS colud be induced by drought stress(15% PEG 6000). The above results indicate that the HaLACS play essential roles in dealing with different abiotic stresses and lipid biosynthesis.

Key words: sunflower, LACS gene family, abiotic stress, gene expression

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