园艺学报 ›› 2022, Vol. 49 ›› Issue (1): 117-128.doi: 10.16420/j.issn.0513-353x.2020-0834
收稿日期:
2021-05-13
修回日期:
2021-09-10
出版日期:
2022-01-25
发布日期:
2022-01-24
通讯作者:
代红军
E-mail:dai_hj@nxu.edu.cn
基金资助:
HE Yan, SUN Yanli, ZHAO Fangfang, DAI Hongjun()
Received:
2021-05-13
Revised:
2021-09-10
Online:
2022-01-25
Published:
2022-01-24
Contact:
DAI Hongjun
E-mail:dai_hj@nxu.edu.cn
摘要:
以酿酒葡萄‘美乐’为试材,于花后8周对其果实喷施不同浓度(0.2、0.4、0.6 mg · L-1)油菜素内酯(2,4-表油菜素内酯,EBR)溶液,以喷施等量蒸馏水为对照,研究 EBR对果实成熟过程中糖组分、糖代谢关键酶及相关基因表达的影响。0.6 mg · L-1 EBR处理促进了果实发育过程中葡萄糖和果糖的积累,花后104 d分别较对照提高了16.95%和39.31%,果实成熟期酸性转化酶(AI)和蔗糖磷酸合成酶(SPS)活性均显著高于对照,果实转色至成熟过程中显著上调了细胞壁酸性转化酶基因(VvcwINV)、蔗糖转运蛋白基因(VvSUC12)、蔗糖合成酶基因(VvSS)的转录水平,花后67 d VvSS的表达量是对照的2.75倍。相关性分析表明,0.6 mg · L-1 EBR处理下果实中己糖含量与SS、AI活性呈显著正相关。0.6 mg · L-1 EBR处理提高了果实SS和AI的活性、促进了VvSS、VvcwINV以及VvSUC12的表达,从而增加了葡萄糖和果糖的含量。0.6 mg · L-1 EBR处理效果最好。
中图分类号:
贺琰, 孙艳丽, 赵芳芳, 代红军. 外源油菜素内酯处理对‘美乐’葡萄果实糖代谢的影响[J]. 园艺学报, 2022, 49(1): 117-128.
HE Yan, SUN Yanli, ZHAO Fangfang, DAI Hongjun. Effect of Exogenous Brassinolides Treatment on Sugar Metabolism of Merlot Grape Berries[J]. Acta Horticulturae Sinica, 2022, 49(1): 117-128.
基因名称 Gene name | 序列号 | 引物序列Primer sequence(5'-3') | |
---|---|---|---|
Accession No. | 正向 Forward | 反向 Reverse | |
SS | BG273882. 1 | CCAGCCAACGTCATTAGCCT | CCATGACTTGTGGCCTTCCT |
SPS | BG273723. 1 | TAGCCCTTGAGGACCCCTAC | CCATGCAAACCGAACCTACCC |
VvcwINV | AY538262 | ATGAATCATCTAGYGTGGAGCAC | CTTAAACGATATCTCCACATCTG |
VVSUC12 | AF021809 | CTTCATCCATTCCTCATCCA | GCGGCAATCATACAACTG |
HT1 | AJ 001061 | GTCTATGTTTCAGGGTTTG | AAGAAGATTTGGGCTATG |
EF | AF176496 | GTTAAGATGATTCCAACCAAGCCC | TCTCCACGCTCTTGATGACTC |
Actin | BN000705 | CTTGCATCCCTCAGCACCTT | TCCTGTGGACAATGGATGGA |
表1 试验所用引物
Table 1 Primers used in the study
基因名称 Gene name | 序列号 | 引物序列Primer sequence(5'-3') | |
---|---|---|---|
Accession No. | 正向 Forward | 反向 Reverse | |
SS | BG273882. 1 | CCAGCCAACGTCATTAGCCT | CCATGACTTGTGGCCTTCCT |
SPS | BG273723. 1 | TAGCCCTTGAGGACCCCTAC | CCATGCAAACCGAACCTACCC |
VvcwINV | AY538262 | ATGAATCATCTAGYGTGGAGCAC | CTTAAACGATATCTCCACATCTG |
VVSUC12 | AF021809 | CTTCATCCATTCCTCATCCA | GCGGCAATCATACAACTG |
HT1 | AJ 001061 | GTCTATGTTTCAGGGTTTG | AAGAAGATTTGGGCTATG |
EF | AF176496 | GTTAAGATGATTCCAACCAAGCCC | TCTCCACGCTCTTGATGACTC |
Actin | BN000705 | CTTGCATCCCTCAGCACCTT | TCCTGTGGACAATGGATGGA |
图1 EBR处理对葡萄果实中葡萄糖(A)、果糖(B)、蔗糖(C)和可溶性总糖(D)含量的影响 不同小写字母表示相同时期处理间差异显著(P < 0.05)。
Fig. 1 Effect of EBR treatments on glucose(A),fructose(B),sucrose(C)and total soluble sugar(D) contents in grape berries Lowercase letters indicate significant differences between treatments in the same period(P < 0.05).
图2 不同浓度EBR处理对葡萄果实蔗糖代谢酶活性的影响 数值表示为平均值 ± 标准误(SD);同列小写字母表示在0.05水平差异显著。
Fig. 2 The effect of different concentration of EBR on sucrose metabolism enzyme activity in grape berries. The value expressed as mean ± standard error(SD);Lowercase letters indicates the difference in the level of P < 0.05 in the same column.
酶 Enzyme | EBR/(mg · L-1) | 相关系数Correlation coefficient | |||
---|---|---|---|---|---|
蔗糖 Sucrose | 葡萄糖 Glucose | 果糖 Fructose | 可溶性总糖 Total soluble sugar | ||
SS | 0(对照 Control) | 0.279 | 0.798 | 0.833* | 0.824* |
0.2 | 0.736 | 0.357 | 0.399 | 0.467 | |
0.4 | 0.766 | 0.504 | 0.468 | 0.460 | |
0.6 | 0.353 | 0.893* | 0.937** | 0.440 | |
NI | 0(对照 Control) | 0.391 | 0.207 | 0.535 | 0.389 |
0.2 | 0.363 | 0.196 | 0.276 | 0.246 | |
0.4 | 0.464 | 0.346 | 0.321 | 0.338 | |
0.6 | 0.569 | 0.244 | 0.283 | 0.409 | |
SPS | 0(对照 Control) | 0.884* | 0.707 | 0.738 | 0.745 |
0.2 | 0.853* | 0.472 | 0.505 | 0.534 | |
0.4 | 0.921** | 0.536 | 0.490 | 0.833* | |
0.6 | 0.962** | 0.562 | 0.640 | 0.860* | |
AI | 0(对照 Control) | 0.640 | 0.458 | 0.469 | 0.676 |
0.2 | 0.313 | 0.722 | 0.755 | 0.809 | |
0.4 | 0.545 | 0.815* | 0.825* | 0.827* | |
0.6 | 0.448 | 0.937** | 0.888* | 0.785 |
表2 葡萄果实糖组分与蔗糖代谢相关酶活性的相关性分析
Table 2 Correlation between sugar components and enzymes activity related to sucrose metabolism in grape berries
酶 Enzyme | EBR/(mg · L-1) | 相关系数Correlation coefficient | |||
---|---|---|---|---|---|
蔗糖 Sucrose | 葡萄糖 Glucose | 果糖 Fructose | 可溶性总糖 Total soluble sugar | ||
SS | 0(对照 Control) | 0.279 | 0.798 | 0.833* | 0.824* |
0.2 | 0.736 | 0.357 | 0.399 | 0.467 | |
0.4 | 0.766 | 0.504 | 0.468 | 0.460 | |
0.6 | 0.353 | 0.893* | 0.937** | 0.440 | |
NI | 0(对照 Control) | 0.391 | 0.207 | 0.535 | 0.389 |
0.2 | 0.363 | 0.196 | 0.276 | 0.246 | |
0.4 | 0.464 | 0.346 | 0.321 | 0.338 | |
0.6 | 0.569 | 0.244 | 0.283 | 0.409 | |
SPS | 0(对照 Control) | 0.884* | 0.707 | 0.738 | 0.745 |
0.2 | 0.853* | 0.472 | 0.505 | 0.534 | |
0.4 | 0.921** | 0.536 | 0.490 | 0.833* | |
0.6 | 0.962** | 0.562 | 0.640 | 0.860* | |
AI | 0(对照 Control) | 0.640 | 0.458 | 0.469 | 0.676 |
0.2 | 0.313 | 0.722 | 0.755 | 0.809 | |
0.4 | 0.545 | 0.815* | 0.825* | 0.827* | |
0.6 | 0.448 | 0.937** | 0.888* | 0.785 |
图3 不同浓度EBR处理对葡萄果实蔗糖代谢相关基因相对表达量的影响 不同小写字母表示显著差异(P < 0.05)。
Fig. 3 The effect of different concentration of EBR on the relative transcript levels of sucrose metabolism in grape berries Different lowercase mean significant differences at 0.05 level.
酶 Enzyme | EBR/(mg · L-1) | 相关系数 Correlation coefficient | ||||
---|---|---|---|---|---|---|
VvSS | VvSPS | VvcwINV | VvSUC12 | VvHT1 | ||
SS | 0 | 0.929** | 0.458 | -0.076 | 0.490 | -0.799 |
0.2 | 0.680 | 0.274 | -0.367 | -0.109 | -0.583 | |
0.4 | 0.553 | 0.395 | 0.416 | 0.542 | 0.153 | |
0.6 | 0.734 | 0.633 | 0.327 | 0.751 | -0.103 | |
NI | 0 | 0.731 | -0.122 | -0.201 | 0.128 | -0.599 |
0.2 | 0.830* | 0.251 | 0.191 | -0.417 | -0.518 | |
0.4 | 0.532 | 0.258 | 0.365 | 0.590 | 0.276 | |
0.6 | 0.420 | 0.454 | 0.746 | 0.504 | -0.186 | |
SPS | 0 | 0.784 | 0.139 | 0.235 | 0.633 | -0.401 |
0.2 | 0.473 | -0.020 | -0.209 | 0.354 | -0.550 | |
0.4 | 0.268 | -0.211 | 0.060 | 0.229 | -0.321 | |
0.6 | 0.132 | -0.010 | 0.011 | 0.189 | -0.647 | |
AI | 0 | 0.767 | 0.061 | 0.225 | 0.800 | -0.393 |
0.2 | 0.477 | -0.056 | 0.135 | -0.278 | -0.832* | |
0.4 | 0.568 | -0.288 | -0.465 | 0.266 | -0.169 | |
0.6 | 0.421 | 0.250 | 0.182 | 0.361 | -0.447 |
表3 蔗糖代谢相关酶活性与蔗糖代谢相关基因相对表达量的相关性
Table 3 Correlation between relative transcript levels and enzymes activity related to sucrose metabolism in grape berries
酶 Enzyme | EBR/(mg · L-1) | 相关系数 Correlation coefficient | ||||
---|---|---|---|---|---|---|
VvSS | VvSPS | VvcwINV | VvSUC12 | VvHT1 | ||
SS | 0 | 0.929** | 0.458 | -0.076 | 0.490 | -0.799 |
0.2 | 0.680 | 0.274 | -0.367 | -0.109 | -0.583 | |
0.4 | 0.553 | 0.395 | 0.416 | 0.542 | 0.153 | |
0.6 | 0.734 | 0.633 | 0.327 | 0.751 | -0.103 | |
NI | 0 | 0.731 | -0.122 | -0.201 | 0.128 | -0.599 |
0.2 | 0.830* | 0.251 | 0.191 | -0.417 | -0.518 | |
0.4 | 0.532 | 0.258 | 0.365 | 0.590 | 0.276 | |
0.6 | 0.420 | 0.454 | 0.746 | 0.504 | -0.186 | |
SPS | 0 | 0.784 | 0.139 | 0.235 | 0.633 | -0.401 |
0.2 | 0.473 | -0.020 | -0.209 | 0.354 | -0.550 | |
0.4 | 0.268 | -0.211 | 0.060 | 0.229 | -0.321 | |
0.6 | 0.132 | -0.010 | 0.011 | 0.189 | -0.647 | |
AI | 0 | 0.767 | 0.061 | 0.225 | 0.800 | -0.393 |
0.2 | 0.477 | -0.056 | 0.135 | -0.278 | -0.832* | |
0.4 | 0.568 | -0.288 | -0.465 | 0.266 | -0.169 | |
0.6 | 0.421 | 0.250 | 0.182 | 0.361 | -0.447 |
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