园艺学报 ›› 2022, Vol. 49 ›› Issue (1): 157-165.doi: 10.16420/j.issn.0513-353x.2020-0758

• 研究报告 • 上一篇    下一篇


李茂福1,2,3, 杨媛1,2,4, 王华1,2,3, 范又维1,2,3, 孙佩1,2,3, 金万梅1,2,3,*()   

  1. 1北京市农林科学院林业果树研究所,北京100093
  • 收稿日期:2021-03-30 修回日期:2021-09-07 出版日期:2022-01-25 发布日期:2022-01-24
  • 通讯作者: 金万梅
  • 基金资助:

Identification and Analysis of Self Incompatibility S-RNase in Rose

LI Maofu1,2,3, YANG Yuan1,2,4, WANG Hua1,2,3, FAN Youwei1,2,3, SUN Pei1,2,3, JIN Wanmei1,2,3,*()   

  1. 1Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China
    2Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture,Beijing 100093,China
    3Beijing Engineering Research Center of Functional Floriculture,Beijing 100093,China
    4Beijing Engineering Research Center for Deciduous Fruit Trees,Beijing 100093,China
  • Received:2021-03-30 Revised:2021-09-07 Online:2022-01-25 Published:2022-01-24
  • Contact: JIN Wanmei


以月季‘月月红’(Rosa chinensis‘Slater’s Crimson China’)和‘2015-58-20’(Rosa sp.)为材料进行自交和异交,授粉花柱中花粉管荧光显微镜观察结果显示月季为典型的配子体自交不亲和植物。通过与已知蔷薇科自交不亲和S-RNase基因的序列进行比对,在‘月月红’中克隆到RcS1-RNaseRcS2-RNase基因的cDNA和DNA全长序列,序列分析表明,RcS1-RNaseRcS2-RNase具有5个保守结构域(C1、C2、C3、RC4和C5)和1个高变区。系统进化分析发现,RcS-RNase与其他蔷薇科植物的S-RNase蛋白具有较高的同源性。组织特异性表达分析表明,RcS1-RNaseRcS2-RNase基因只在花柱中特异表达。

关键词: 月季, 自交不亲和, S-RNase, 基因克隆, 基因表达


In Rosa chinensis‘Slater’s Crimson China’and Rosa sp.‘2015-58-20’,the fluorescence microscope observation of pollen tubes in both self-pollinated and cross-pollinated styles indicated that rose is a typical gametophyte self-incompatible plant. The full-length sequence of RcS1-RNase and RcS2-RNase cDNA and DNA have been identified in the‘Slater’s Crimson China’. Sequence analysis showed that RcS1-RNase and RcS2-RNase have five conserved domains(C1,C2,C3,RC4 and C5)and a hypervariable region. Phylogenetic analysis found that RcS-RNase has high homology with S-RNase proteins in other Rosaceae plants. Tissue specific expression analysis indicated that RcS1-RNase and RcS2-RNase genes are solely expressed in styles.

Key words: Rosa, self incompatibility, S-RNase, gene cloning, gene expression