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园艺学报 ›› 2019, Vol. 46 ›› Issue (11): 2213-2223.doi: 10.16420/j.issn.0513-353x.2019-0065

• 研究论文 • 上一篇    下一篇

γ–氨基丁酸诱导匍匐翦股颖耐铝性生理及分子机制初探

曾伟航,李 州*   

  1. 四川农业大学动物科技学院草学系,成都 611130
  • 出版日期:2019-11-25 发布日期:2019-11-25
  • 基金资助:
    国家自然科学基金项目(31702182)

The Study on Physiological and Molecular Mechanism of Aluminum Tolerance Induced By Exogenous γ-Aminobutyric Acid in Creeping Bentgrass

ZENG Weihang and LI Zhou*   

  1. College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China
  • Online:2019-11-25 Published:2019-11-25

摘要: 研究了γ–氨基丁酸(GABA)对铝(AlCl3)胁迫下‘Pencross’匍匐翦股颖幼苗叶片叶绿素含量、相对含水量、渗透调节、活性氧成分、蛋白质羰基含量、细胞膜稳定性、抗氧化防御以及抗逆相关基因差异表达的影响。外源添加0.5 mmol ? L -1 GABA预处理能显著提高铝胁迫下匍匐翦股颖叶片叶绿素含量和相对含水量;诱导可溶性蛋白、可溶性糖和游离脯氨酸的积累,降低细胞渗透势,增强细胞渗透调节能力;同时增强铝胁迫下关键抗氧化酶过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性及相关基因表达,抑制铝胁迫下细胞内活性氧、电解质渗透率、丙二醛(MDA)和蛋白质羰基含量的上升,有效缓解了铝胁迫对细胞造成的氧化伤害。此外,外源GABA预处理也显著诱导铝胁迫下脱水蛋白基因DHN3的表达。表明GABA显著提高匍匐翦股颖耐铝性,促进有机渗透调节物质积累,提高渗透调节能力,增强抗氧化防御系统,有效缓解氧化伤害。GABA诱导脱水蛋白基因(DHN3)表达,提示其可能参与GABA提高匍匐翦股颖的耐铝性。

关键词: 匍匐翦股颖, γ–氨基丁酸, 铝胁迫, 渗透调节, 抗氧化, 基因表达, 脱水蛋白, 金属硫蛋白

Abstract: Effects of γ-aminobutyric acid(GABA)on chlorophyll content,relative water content,osmotic adjustment,reactive oxygen accumulation,protein carbonyl content,cell membrane stability,antioxidant defense,and differential expression of stress-related genes in leaves of creeping bentgrass (Agrostis stolonifera)‘Pencross’seedlings exposed to aluminum(AlCl3)stress were studied. Exogenous application of 0.5 mmol ? L -1 GABA could significantly increase chlorophyll content and relative water content and induce accumulation of soluble protein,soluble sugar,and free proline leading to the decrease in cell osmotic potential and increase in osmotic adjustment in leaves of creeping bentgrass under aluminum stress. Exogenous GABA also could significantly improve activities of key antioxidant enzymes CAT,POD,and SOD as well as their genes expression levels associated with the significantly lower reactive oxygen accumulation,electrolyte leakage,malondialdehyde content,and protein carbonyl content in leaves under aluminum stress,hence the application of GABA effectively alleviated the oxidative damage caused by aluminum stress. Exogenous GABA also promoted the creeping creeping growth under normal condition. In addition,exogenous GABA significantly induced the expression of the dehydrins-encoded gene DHN3 under aluminum stress. These results showed that GABA significantly increased aluminum tolerance of creeping bentgrass associated with the accumulation of organic osmolytes and enhancement of osmotic adjustment and antioxidant defense,thereby effectively alleviating oxidative damage under aluminum stress. GABA-induced dehydrins-encoded gene DHN3 expression may be also involved in GABA- regulated aluminum tolerance in creeping bentgrass.

Key words: Agrostis stolonifera, γ-aminobutyric acid, aluminum stress, osmotic adjustment, antioxidant, gene expression, dehydrins, metallothionein

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