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园艺学报 ›› 2019, Vol. 46 ›› Issue (11): 2201-2212.doi: 10.16420/j.issn.0513-353x.2019-0450

• 研究论文 • 上一篇    下一篇

切花菊耐寒性相关SNP位点挖掘与候选基因分析

范宏虹,徐婷婷,苏江硕,孙 炜,种昕冉,管志勇,房伟民,陈发棣,张 飞*   

  1. 南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,南京 210095
  • 出版日期:2019-11-25 发布日期:2019-11-25
  • 基金资助:
    国家自然科学基金项目(31572152);江苏省重点研发计划项目(BE2018426);江苏省优势学科建设工程项目

Identification of SNP Alleles and Candidate Genes for Cold Tolerance of Cut Chrysanthemum

FAN Honghong,XU Tingting,SU Jiangshuo,SUN Wei,CHONG Xinran,GUAN Zhiyong,FANG Weimin,CHEN Fadi,and ZHANG Fei*   

  1. College of Horticulture,Nanjing Agricultural University,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095,China
  • Online:2019-11-25 Published:2019-11-25

摘要: 以58份切花菊为试验材料,调查了脚芽期、现蕾期和盛花期叶片以及盛花期舌状花低温半致死温度(LT50),利用全基因组关联分析挖掘耐寒性相关优异等位变异和候选基因。结果表明,切花菊品种不同时期叶片或盛花期舌状花耐寒性变异较大,变异系数均大于25%;通过主效可加互作可乘(AMMI)模型的双标图分析筛选出抗寒性强且稳定性较好的品种‘天使’。基于36 737个高质量SNP位点和混合线性模型的关联分析,共挖掘出24个与耐寒性显著关联的SNP位点(P < 0.001),其中有5个、11个和5个SNP位点分别与脚芽期、现蕾期、盛花期叶片耐寒性相关,3个SNP位点与盛花期舌状花耐寒性相关;5个SNP位点的表型效应值小于–4 ℃,为优异等位变异位点。通过显著性SNP位点所在的SLAF标签序列与菊花转录组数据库进行比对分析,筛选得到5个候选基因,其中CL2042.Contig4_All 和Unigene40993_All可能与冷胁迫有一定关联。

关键词: 菊花, 耐寒性, 全基因组关联分析, 候选基因

Abstract: In this study,the low semi-lethal temperature(LT50)was investigated for leaves at rhizome stage(RS),budding stage(RS),and flowering stage(FS),and ray florets at flowering stage(RFS)of 58 cut chrysanthemum accessions,and elite alleles and candidate genes relevant to cold tolerance were examined using genome-wide association analysis. The results showed that,the cold tolerance varied widely for leaves at different stages and ray florets at the flowering stage in the investigated cut chrysanthemums,with coefficients of variation larger than 25%. Statistical analysis by the additive main effects and multiplicative interaction effects(AMMI)model revealed that several accessions showed stronger cold tolerance and expressed stably across different stages. The genome-wide association analysis based on 36 737 high-quality SNPs and mixed linear model(MLM)identified a total of 24 SNPs that are significantly associated with cold tolerance,each five for RS and FS,11 for BS,and three for RFS. Among which,five SNPs showing a phenotypic effect <–4 ℃ should be elite alleles for cold tolerance. Blasting the SLAF tags harboring the cold tolerance-associated SNPs with the transcriptomes of chrysanthemum,CL2042.Contig4_All and Unigene40993_All might function in tolerance to cold stress.

Key words: chrysanthemum, cold tolerance, genome-wide association analysis, candidate genes

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