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园艺学报 ›› 2021, Vol. 48 ›› Issue (8): 1446-1456.doi: 10.16420/j.issn.0513-353x.2020-0630

• 研究论文 • 上一篇    下一篇

甜樱桃磷酸蔗糖合酶基因PavSPS的功能分析

齐希梁, 刘聪利, 宋露露, 李明()   

  1. 中国农业科学院郑州果树研究所,郑州 450009
  • 收稿日期:2020-12-10 修回日期:2021-04-26 出版日期:2021-08-25 发布日期:2021-09-06
  • 通讯作者: 李明 E-mail:liming06@caas.cn
  • 基金资助:
    中国农业科学院科技创新工程专项(CAAS-ASTIP-2020-ZFRI);河南省自然科学基金项目(20230041053);河南省自然科学基金项目(212300410419)

Functional Analysis of Sucrose-phosphate Synthase Genes(SPS)in Sweet Cherry

QI Xiliang, LIU Congli, SONG Lulu, LI Ming()   

  1. Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China
  • Received:2020-12-10 Revised:2021-04-26 Online:2021-08-25 Published:2021-09-06
  • Contact: LI Ming E-mail:liming06@caas.cn

摘要:

从甜樱桃基因组中鉴定了4个磷酸蔗糖合酶(sucrose phosphate synthase,SPS)基因(PavSPSA1PavSPSA2PavSPSBPavSPSC)。实时荧光定量PCR分析表明,PavSPSA1在甜果实发育和成熟过程中表达量最高,其次是PavSPSC,PavSPSA2PavSPSB表达量较低;PavSPSA1PavSPSA2在果实成熟软化过程中均上调表达,与甜樱桃果实的成熟软化过程吻合。通过利用病毒诱导的基因沉默(VIGS)技术沉默PavSPSA1,甜樱桃果实蔗糖含量降低,果实着色和果实成熟软化延迟;沉默PavSPSA2PavSPSBPavSPSC,甜樱桃果实蔗糖含量、果实着色和果实成熟软化与对照无差异,推测PavSPSA1或许是调控果实蔗糖合成与积累的关键基因,影响果实着色和成熟软化。

关键词: 甜樱桃, 磷酸蔗糖合酶, PavSPSA1, 基因表达, 果实成熟, 基因沉默

Abstract:

In order to ascertain the function of sucrose-phosphate synthase genes(SPS)in the regulation of sweet cherry ripening,four full-length cDNA of sweet cherry SPS genes(PavSPSA1,PavSPSA2,PavSPSB,and PavSPSC)were cloned in sweet cherry genome. Gene expression analysis showed that the expression levels of PavSPSA1 was the highest,followed by PavSPSC,whereas PavSPSA2 and PavSPSB were relatively weakly expressed during the fruit growth and development,and ripening using quantitative real-time PCR. The expression levels of PavSPSA1 and PavSPSA2 coincided with fruit ripening and softening. Furthermore,we used TRV-mediated virus-induced gene silencing(VIGS)to evaluate the function of PavSPSA1,PavSPSA2,PavSPSB,and PavSPSC in the regulation of ripening. The results showed that silencing PavSPSA1 gene decreased sucrose content,delayed fruit coloring,ripening and softening. However,The fruit phenotypes for the PavSPSA2,PavSPSB,and PavSPSC-silenced fruits showed no visible defects when compared with the control fruits. Taken together,our findings determined that PavSPSA1 may be a key gene regulating sucrose synthesis and accumulation in sweet cherry fruit and play an indispensable role in the regulation of sweet cherry fruit ripening and softening.

Key words: sweet cherry, sucrose-phosphate synthase, PavSPSA1, gene expression, fruit ripening, VIGS

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