ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (4): 641-649.doi: 10.16420/j.issn.0513-353x.2017-0578

• Research Papers • Previous Articles     Next Articles

Analysis of the Complete Genome of Apple stem grooving virus Isolate Jilin-shaguo

LI Zhengnan,ZHANG Shuangna,ZHANG Zunping,FAN Xudong,REN Fang,HU Guojun,and DONG Yafeng*   

  1. National Center for Eliminating Viruses from Deciduous Fruit Tree,Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China
  • Online:2018-04-25 Published:2018-04-25


Using RT-PCR and RACE techniques,the complete genome of Apple stem grooving virus(ASGV)isolate Jilin-shaguo(JLSG)was amplified. The genome of ASGV-JLSG has 6 496 nucleotides,encoding two overlapping open reading frames(ORFs). The ORF1(position 37–6 354 nt)encodes a 241 kD polyprotein,including domains of a methyltransferase,a papain-like-protease,a nucleotide triphosphate-binding helicase,a RNA-dependent RNA polymerase,and a coat protein. ORF2(4 788– 5 750 nt)encodes a movement protein. The nucleotide identity of complete genome between ASGV-JLSG and the 29 ASGV isolates published in GenBank ranged from 79.20% to 86.60%. Phylogenetic analysis indicated that the concerned ASGV isolates can be grouped into 2 clusters,but no geographic or host association among one another. Recombination analysis suggested that ASGV-JLSG was the recombinant of ASGV-HH(JN701424)and CTLV-MTH(KC588948). This was the first reported complete genome of ASGV in Northeast China.

Key words: apple, Apple stem grooving virus, genome, phylogenetic analysis, identity, recombination

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