Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 65-78.doi: 10.16420/j.issn.0513-353x.2021-0985

• Research Papers • Previous Articles     Next Articles

Construction of Cabbage Chromosome Segment Substitution Lines

CUI Jian1,2, ZHONG Xionghui2, LIU Zeci2, CHEN Denghui2, LI Hailong2, HAN Rui2, YUE Xiangqing2, KANG Jungen2,*(), WANG Chao1,*()   

  1. 1School of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China
    2Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China
  • Received:2022-08-17 Revised:2022-11-17 Online:2023-01-25 Published:2023-01-18
  • Contact: *(


In this experiment,cabbage R2-P2,a foundation parent with head,late bolting and low glucosinolate content,high susceptibility to black rot,Fusarium wilt and downy mildew was served as the recurrent parent. On the contrary,the donor parent R4P1 is a wild cabbage inbred line R4-P1 with no-heading,early bolting,thick wax,high glucosinolate content,and resistance to black rot,Fusarium wilt,downy mildew and other diseases. Resultly,the Chromosome segment substitution lines(CSSLs)was both possessed the genetic background of R2-P2 and individual donor fragment of R4-P1. The CSSLs with 163 lines was constructed by continuous backcross,selfing and marker-assisted selection. The CSSLs contained 102 substituted segments totally,which were derived from genome of donor parent R4-P1 and evenly distributed on nine chromosomes. There were average 1.3 chromosome fragments on a line at least. The whole length of the substituted segments was 573.74 Mb with an average length of 5.62 Mb,which covered the 73.4% of complete DNA sample of R4-P1. Among them,139.91 Mb of single substituted segments covered the 26.5% of complete DNA. Besides,the double-segment replacement fragments were 231.41 Mb,which was accounted for 43.82%. Meanwhile,the total length of three replacement fragments was 201.3 Mb,covering 25.98% of the donor parent genome.

Key words: cabbage, Chromosome segment substitution line, genome, molecular marker, QTL

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