Abstract:

Using RT-PCR and RACE techniques，the complete genome of Apple stem grooving virus（ASGV）isolate Jilin-shaguo（JLSG）was amplified. The genome of ASGV-JLSG has 6 496 nucleotides，encoding two overlapping open reading frames（ORFs）. The ORF1（position 37–6 354 nt）encodes a 241 kD polyprotein，including domains of a methyltransferase，a papain-like-protease，a nucleotide triphosphate-binding helicase，a RNA-dependent RNA polymerase，and a coat protein. ORF2（4 788– 5 750 nt）encodes a movement protein. The nucleotide identity of complete genome between ASGV-JLSG and the 29 ASGV isolates published in GenBank ranged from 79.20% to 86.60%. Phylogenetic analysis indicated that the concerned ASGV isolates can be grouped into 2 clusters，but no geographic or host association among one another. Recombination analysis suggested that ASGV-JLSG was the recombinant of ASGV-HH（JN701424）and CTLV-MTH（KC588948）. This was the first reported complete genome of ASGV in Northeast China.

Key words: apple, Apple stem grooving virus, genome, phylogenetic analysis, identity, recombination