Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1977-1990.doi: 10.16420/j.issn.0513-353x.2021-0378

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Comparative Chloroplast Genome Study of Mallus servisii‘Red Delicious’and‘Golden Delicious’

DING Zhijie, BAO Jinbo, ROUXIAN Guli, ZHU Tiantian, LI Xueli, MIAO Haoyu, TIAN Xinmin*()   

  1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Sciences and Technology,Xinjiang University,Urumqi 830046,China
  • Received:2022-02-17 Revised:2022-05-19 Online:2022-09-25 Published:2022-10-08
  • Contact: TIAN Xinmin


The wild apple tree Malus sieversii(Rosaceae)is considered to be the ancestor of modern cultivated apple trees. To effectively conserve apple genetic resources,it would be important to elucidate differences of chloroplast genomes between M. sieversii and cultivated apples,as well as verify whether modern cultivated apples originated from M. sieversii. In this study,we used the next-generation sequencing technology to complete the whole-genome sequencing,assembly,and annotation of M. sieversii as well as two domesticated apple trees‘Red Delicious’and‘Golden Delicious’. We successfully mapped the chloroplast genomes of M. sieversii and the two domesticated apple trees,and subsequently constructed the Malus phylogenetic tree at the level of the chloroplast genome. Through a series of comparative analyses,We obtained the following main results:the chloroplast genome of M. sieversii exhibites a typical quadripartite structure,and the complete genomes ranged between 160 068 and 160 288 bp in length. A total of 131 genes were annotated in M. sieversii and the two domesticated apple trees,including 86 protein-coding,37 tRNA,and eight rRNA genes. The base composition of the chloroplast genomes of M. sieversii and the two domesticated apples varieties were similar. A total of 43,49,and 43 repetitive sequence and 64,61 and 57 simple sequence repeat sites were identified in M. sieversii,‘Red Delicious’and‘Golden Delicious’,respectively. For the simple sequence repeats,the main repeat types were single A or T base repeats. Finally,phylogeny study showed that all the Malus species formed a single monophyletic group(BP = 100). M. sieversii and the two cultivated varieties were grouped in a subclade with strong support(BP = 100),supporting the hypothesis that M. sieversii is the putative ancestor of domesticated apples.

Key words: Malus sieversii, chloroplastgenome, variation analysis, phylogeny

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