Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 161-169.doi: 10.16420/j.issn.0513-353x.2021-0756

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Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Pepper Isolate in Qinghai Province

LUO Hailin1, YUAN Lei1, WENG Hua1, YAN Jiahui1,*(), GUO Qingyun1,*(), WANG Wenqing2, MA Xinming3   

  1. 1Key Laboratory of Agricultural Integrated Pest Management,Scientific Observing and Experimental Station of Crop Pest in Xining,Ministry of Agriculture and Rural Affairs,Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,China
    2Vegetable Technical Service Center of Datong County,Xining 810016,China
    3Bureau of Agriculture and Rural & Science and Technology Affairs of Xunhua County,Haidong,Qinghai 810600,China
  • Received:2022-05-12 Revised:2022-08-13 Online:2023-01-25 Published:2023-01-18
  • Contact: *(


An investigation on pepper virus was conducted. Samples collected in Xunhua County,Qinghai Province shown symptoms of plant wilt and leaf shrinkage,which were very similar to the symptoms induced by broad bean wilt virus 2(BBWV2). To identify the possible virus associated with the disease,20 samples were collected and detected with universal primer and specific primer using RT-PCR

and 13 samples shown positive results when using a pair of degenerate primers for BBWV2. The results showed that 13 of 20 pepper samples in Xunhua County were confirmed to be infected with broad bean wilting virus 2. Further phylogenetic analysis based on the nucleotide sequence also suggested that the virus detected was clustered with BBWV2 and shared the highest sequence identities with the isolates from abroad,and relatively lower identities with the isolates of broad bean wilt virus 1(BBWV1)or Lamium mild mosaic virus(LMMV). All these results showed that the virus infected pepper is an isolate of BBWV2.

Key words: pepper, broad bean wilt virus, whole genome

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