Effect of Exogenous Brassinolides Treatment on Sugar Metabolism of Merlot Grape Berries

doi:10.16420/j.issn.0513-353x.2020-0834

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 117-128.doi: 10.16420/j.issn.0513-353x.2020-0834

• Research Notes • Previous Articles Next Articles

Effect of Exogenous Brassinolides Treatment on Sugar Metabolism of Merlot Grape Berries

HE Yan, SUN Yanli, ZHAO Fangfang, DAI Hongjun()

College of Agronomy,Ningxia University,Yinchuan 750021,China

Received:2021-05-13 Revised:2021-09-10 Online:2022-01-25 Published:2022-01-24
Contact: DAI Hongjun E-mail:dai_hj@nxu.edu.cn

Abstract

Abstract:

The purpose of this study was to explore the effect of exogenous brassinolides（2,4- Epibrassinolide,EBR）on sugar metabolism of Merlot grape berries. 10-year-old grape Vitis vinifera ‘Merlot’was used as the test material,and different concentrations of EBR（0.2,0.4,0.6 mg · L^-1）solutions were sprayed in the eighth week after anthesis,with distilled water as the control. Using chemical analysis,HPLC,Enzyme-linked Immunosorbent assay,and transcription analysis to study the effects of EBR treatment on the sugar components,key sugar metabolism enzymes and related gene expression during the ripening process of‘Merlot’grapes. Compared with the control,0.6 mg · L^-1 EBR treatment can significantly promote the accumulation of glucose and fructose in grape berries,the contents were respectively increased by 16.95% and 39.31%. At maturation,the activities of acid invertase（AI）and sucrose phosphate synthase（SPS）were significantly higher than the control. The 0.6 mg · L^-1 EBR significantly up-regulated the transcription levels of cell wall acid invertase（VvcwINV）,sucrose transporter（VvSUC12）,and sucrose synthetase（VvSS）during the process of grape color transformation to maturity. The expression of VvSS gene was 2.75 times that of the control. Correlation analysis showed that hexose was significantly positively correlated with SS and AI under 0.6 mg · L^-1 EBR treatment. 0.6 mg · L^-1 EBR treatment improves the activity of SS and AI,and promotes the expression of VvSS,VvcwINV gene and sucrose transporter VvSUC12 gene,thereby increasing the content of glucose and fructose. 0.6 mg · L^-1 EBR treatment has the best effect.

Key words: grape, EBR, sugar metabolism, gene expression

CLC Number:

S663.1

HE Yan, SUN Yanli, ZHAO Fangfang, DAI Hongjun. Effect of Exogenous Brassinolides Treatment on Sugar Metabolism of Merlot Grape Berries[J]. Acta Horticulturae Sinica, 2022, 49(1): 117-128.

Figures/Tables 6

References 36

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基因名称 Gene name	序列号	引物序列Primer sequence（5'-3'）
基因名称 Gene name	Accession No.	正向 Forward	反向 Reverse
SS	BG273882. 1	CCAGCCAACGTCATTAGCCT	CCATGACTTGTGGCCTTCCT
SPS	BG273723. 1	TAGCCCTTGAGGACCCCTAC	CCATGCAAACCGAACCTACCC
VvcwINV	AY538262	ATGAATCATCTAGYGTGGAGCAC	CTTAAACGATATCTCCACATCTG
VVSUC12	AF021809	CTTCATCCATTCCTCATCCA	GCGGCAATCATACAACTG
HT1	AJ 001061	GTCTATGTTTCAGGGTTTG	AAGAAGATTTGGGCTATG
EF	AF176496	GTTAAGATGATTCCAACCAAGCCC	TCTCCACGCTCTTGATGACTC
Actin	BN000705	CTTGCATCCCTCAGCACCTT	TCCTGTGGACAATGGATGGA

基因名称 Gene name	序列号	引物序列Primer sequence（5'-3'）
基因名称 Gene name	Accession No.	正向 Forward	反向 Reverse
SS	BG273882. 1	CCAGCCAACGTCATTAGCCT	CCATGACTTGTGGCCTTCCT
SPS	BG273723. 1	TAGCCCTTGAGGACCCCTAC	CCATGCAAACCGAACCTACCC
VvcwINV	AY538262	ATGAATCATCTAGYGTGGAGCAC	CTTAAACGATATCTCCACATCTG
VVSUC12	AF021809	CTTCATCCATTCCTCATCCA	GCGGCAATCATACAACTG
HT1	AJ 001061	GTCTATGTTTCAGGGTTTG	AAGAAGATTTGGGCTATG
EF	AF176496	GTTAAGATGATTCCAACCAAGCCC	TCTCCACGCTCTTGATGACTC
Actin	BN000705	CTTGCATCCCTCAGCACCTT	TCCTGTGGACAATGGATGGA

酶 Enzyme	EBR/（mg · L^-1）	相关系数Correlation coefficient
酶 Enzyme	EBR/（mg · L^-1）	蔗糖 Sucrose	葡萄糖 Glucose	果糖 Fructose	可溶性总糖 Total soluble sugar
SS	0（对照 Control）	0.279	0.798	0.833^*	0.824^*
	0.2	0.736	0.357	0.399	0.467
	0.4	0.766	0.504	0.468	0.460
	0.6	0.353	0.893^*	0.937^**	0.440
NI	0（对照 Control）	0.391	0.207	0.535	0.389
	0.2	0.363	0.196	0.276	0.246
	0.4	0.464	0.346	0.321	0.338
	0.6	0.569	0.244	0.283	0.409
SPS	0（对照 Control）	0.884^*	0.707	0.738	0.745
	0.2	0.853^*	0.472	0.505	0.534
	0.4	0.921^**	0.536	0.490	0.833^*
	0.6	0.962^**	0.562	0.640	0.860^*
AI	0（对照 Control）	0.640	0.458	0.469	0.676
	0.2	0.313	0.722	0.755	0.809
	0.4	0.545	0.815^*	0.825^*	0.827^*
	0.6	0.448	0.937^**	0.888^*	0.785

酶 Enzyme	EBR/（mg · L^-1）	相关系数Correlation coefficient
酶 Enzyme	EBR/（mg · L^-1）	蔗糖 Sucrose	葡萄糖 Glucose	果糖 Fructose	可溶性总糖 Total soluble sugar
SS	0（对照 Control）	0.279	0.798	0.833^*	0.824^*
	0.2	0.736	0.357	0.399	0.467
	0.4	0.766	0.504	0.468	0.460
	0.6	0.353	0.893^*	0.937^**	0.440
NI	0（对照 Control）	0.391	0.207	0.535	0.389
	0.2	0.363	0.196	0.276	0.246
	0.4	0.464	0.346	0.321	0.338
	0.6	0.569	0.244	0.283	0.409
SPS	0（对照 Control）	0.884^*	0.707	0.738	0.745
	0.2	0.853^*	0.472	0.505	0.534
	0.4	0.921^**	0.536	0.490	0.833^*
	0.6	0.962^**	0.562	0.640	0.860^*
AI	0（对照 Control）	0.640	0.458	0.469	0.676
	0.2	0.313	0.722	0.755	0.809
	0.4	0.545	0.815^*	0.825^*	0.827^*
	0.6	0.448	0.937^**	0.888^*	0.785

酶 Enzyme	EBR/（mg · L^-1）	相关系数 Correlation coefficient
酶 Enzyme	EBR/（mg · L^-1）	VvSS	VvSPS	VvcwINV	VvSUC12	VvHT1
SS	0	0.929^**	0.458	-0.076	0.490	-0.799
	0.2	0.680	0.274	-0.367	-0.109	-0.583
	0.4	0.553	0.395	0.416	0.542	0.153
	0.6	0.734	0.633	0.327	0.751	-0.103
NI	0	0.731	-0.122	-0.201	0.128	-0.599
	0.2	0.830^*	0.251	0.191	-0.417	-0.518
	0.4	0.532	0.258	0.365	0.590	0.276
	0.6	0.420	0.454	0.746	0.504	-0.186
SPS	0	0.784	0.139	0.235	0.633	-0.401
	0.2	0.473	-0.020	-0.209	0.354	-0.550
	0.4	0.268	-0.211	0.060	0.229	-0.321
	0.6	0.132	-0.010	0.011	0.189	-0.647
AI	0	0.767	0.061	0.225	0.800	-0.393
	0.2	0.477	-0.056	0.135	-0.278	-0.832^*
	0.4	0.568	-0.288	-0.465	0.266	-0.169
	0.6	0.421	0.250	0.182	0.361	-0.447

Effect of Exogenous Brassinolides Treatment on Sugar Metabolism of Merlot Grape Berries

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