The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes

doi:10.16420/j.issn.0513-353x.2021-0141

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 723-738.doi: 10.16420/j.issn.0513-353x.2021-0141

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The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes

LI Shasha¹, YU Saisai¹, FU Yuheng¹, LUO Qiangwei², XU Yan¹, WANG Yuejin¹^,^**()

1. State Key Laboratory of Crop Stress Biology in Arid Areas,Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest,Ministry of Agriculture and Rural Affairs,College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100,China
2. Institute of Grapes and Melons on the Research in Xinjiang, Shanshan,Xinjiang 838200,China

Received:2021-11-15 Revised:2022-01-18 Online:2022-04-25 Published:2022-04-24
Contact: WANG Yuejin E-mail:wangyj@nwsuaf.edu.cn

Abstract

Abstract:

New germplasms of seedless and cold-resistant grapes were cultivated by embryo rescue techniques after crossing in the field and by molecular marker-assisted selection using stenospermocarpic seedless grapes as the female parents and varieties of Chinese cold-resistant wild species or hybrids from Vitis vinifera × V. amurensis as the male parents. Among the 14 hybrid combinations,a total of 1 021 hybrid plantlets were obtained,among which 634 plantlets were obtained in the combination of‘Ruby Seedless’×‘Beibinghong’with the highest seedling rate of 18.13%. When‘Zuoyouhong’was the male parent,‘Flame Seedless’was suitable as female parent. When‘Beibinghong’and‘Xuelanhong’were as the male parent,‘Ruby Seedless’was suitable as female parent. When‘Beichun’as the male parent,‘Kunxiang Seedless’was suitable as female parent. A total of 132 lines related to seedlessness preliminary screened by the two seedless molecular markers SCF27 and GSLP1. A total of 190 lines related to cold-resistant were initially screened through the molecular marker S241-717. Among these hybrid progeny screened,59 lines possessed both seedless and cold-resistant traits. The grapevine cold-related genes CBF1,COR27 and ERD15 detected by qRT-PCR in the lines with or without cold-resistant traits of the hybrid progeny of‘Ruby Seedless’×‘Beibinghong’and‘Ruby Seedless’×‘Xuelanhong’under cold treatment were differential expression,and the cold-related lines showed significant expression of the cold-resistant genes.

Key words: grape, seedlessness, cold-resistance, embryo rescue, molecular marker

CLC Number:

S663.1

LI Shasha, YU Saisai, FU Yuheng, LUO Qiangwei, XU Yan, WANG Yuejin. The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes[J]. Acta Horticulturae Sinica, 2022, 49(4): 723-738.

Figures/Tables 10

References 58

[1]	Agüero C B, Riquelme C, Tizio R. 1995. Embryo rescue from seedless grapevines(Vitis vinifera L.)treated with growth retardants. Vitis, 34 (2):73-76.
[2]	Aalto M K, Heleniusa E, Kariolaa T, Pennanen V, Heino P, Horak H, Puzorjova I, Kollist H, Palva E T. 2012. ERD15-an attenuator of plant ABA responses and stomatal aperture. Plant Science, 182 (1):19-28. doi: 10.1016/j.plantsci.2011.08.009 URL
[3]	Amaral A L D, Oliveira P R D D, Czermainski A B C, Camargo U A. 2001. Embryo growth stages on plant obtention from crosses between seedless grape parents. Revista Brasileira de Fruticultura, 23 (3):647-651. doi: 10.1590/S0100-29452001000300041 URL
[4]	Bharathy P V, Karibasappa G S. 2003. Influence of pre-bloom sprays of benzyladenine on in vitro recovery of hybrid embryos from crosses of Thompson Seedless and 8 seeded varieties of grape(Vitis spp.). Vitis, 42 (4):199-202.
[5]	Cain D. 2010. New diversity in table grapes:a commercial perspective. Journal of the American Pomological Society, 64 (2):83-85.
[6]	Cain D W, Emershad R L. 1983. In-ovulo embryo culture and seedling development of seeded and seedless grapes(Vitis vinifera L.). Vitis, 22:9-14.
[7]	Chang D, Zhen Z, Yang Q, Ren J, Huang J, Wang B, Lu H, Cai B, Tao J. 2013. VaCBF1 from Vitis amurensis associated with cold acclimation and cold tolerance. Acta Physiologiae Plantarum, 35 (10):2975-2984. doi: 10.1007/s11738-013-1329-3 URL
[8]	Ding Lan. 2019. Analysis of cold resistance function of VaSAP15 gene in Vitis amurensis [M. D. Dissertation]. Yangling:Northwest A & F University. (in Chinese)
	丁兰. 2019. 中国野生山葡萄VaSAP15基因的抗寒功能分析[硕士论文]. 杨凌:西北农林科技大学.
[9]	Ebadi A, Sarikhani H, Zamani Z, Babalar M. 2004. Effect of male parent and application of boric acid on embryo rescue in some seedless grapevine(Vitis vinifera)cultivars. Acta Horticulturae,(640):255-260.
[10]	Emershad R L, Ramming D W, Serpe M D. 1989. In ovulo embryo development and plant formation from stenospermic genotypes of Vitis vinifera. American Journal of Botany, 76 (3):397-402. doi: 10.1002/j.1537-2197.1989.tb11327.x URL
[11]	Fan Gao-tao, Sun Xiao-ming, Ren Xiao-die, Li Shao-hua, Xin Hai-ping, Wang Wan-jun. 2015. Cloning and cold resistance function of COR27 gene in grape. Plant Science Journal, 33 (3):346-354. (in Chinese)
	范高韬, 孙小明, 任小蝶, 李绍华, 辛海平, 王万军. 2015. 葡萄COR27基因的克隆与抗寒功能研究. 植物科学学报, 33 (3):346-354.
[12]	Gribaudo I, Zanetti R, Botta R, Vallania R, Eynard I. 1993. In ovulo embryo culture of stenospermocarpic grapes. Vitis, 32:9-14.
[13]	Guo Yin-shan, Guo Xiu-wu, Zhang Hai-e, Li Yi-hui, Li Cheng-xiang. 2006. Studies on the factors affecting embryo rescue of the crossed progeny between diploid and tetraploid grape cultivars. Journal of Fruit Science, 23 (1):115-117. (in Chinese)
	郭印山, 郭修武, 张海娥, 李轶晖, 李成祥. 2006. 葡萄二倍体和四倍体杂交胚挽救的影响因素. 果树学报, 23 (1):115-117.
[14]	He Pu-chao, Chao Wu-ji. 1982. Studies on the cold hardiness of eight species of Vitis L. growing wild in China. Acta Horticulturae Sinica, 9 (3):17-21. (in Chinese)
	贺普超, 晁无疾. 1982. 我国葡萄属野生种质资源的抗寒性分析. 园艺学报, 9 (3):17-21.
[15]	Ji W, Wang Y. 2013. Breeding for seedless grapes using Chinese wild Vitis spp. II. In vitro embryo rescue and plant development. Journal of the Science of Food and Agricultural, 93 (15):3870-3875. doi: 10.1002/jsfa.6342 URL
[16]	Jia Shan-shan, Luo Qiang-wei, Li Sha-sha, Wang Yue-jin. 2020. Optimization of embryo rescue technique and production of potential seedless grape germplasm with rosy aroma. Scientia Agricultura Sinica, 53 (16):3344-3355. (in Chinese)
	贾姗姗, 骆强伟, 李莎莎, 王跃进. 2020. 葡萄胚挽救技术优化及无核和玫瑰香味新种质创制. 中国农业科学, 53 (16):3344-3355.
[17]	Kariola T, Brader G, Helenius E, Li J, Heino P, Palva E T. 2006. Early responsive to dehydration 15,a negative regulator of abscisic acid responses in Arabidopsis. Plant Physiology, 142 (4):1559-1573. pmid: 17056758
[18]	Lahogue F, This P, Bouquet A. 1998. Identification of a codominant scar marker linked to the seedlessness character in grapevine. Theoretical and Applied Genetics, 97 (5-6):950-959. doi: 10.1007/s001220050976 URL
[19]	Ledbetter C A, Shonnard C B. 1990. Improved seed development and germination of stenospermic grapes by plant growth regulators. Journal of Horticultural Science, 65 (3):269-274. doi: 10.1080/00221589.1990.11516056 URL
[20]	Li G, Ji W, Wang G, Zhang J, Wang Y. 2014. An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species. In Vitro Cellular & Development Biology - Plant, 50 (1):110-120.
[21]	Li Jin-yue, Wang Fei, Wang Yue-jin, Wei Yan-xia. 2008. Study on the freezing resistance in embryo rescue hybrids of seedless and seeded grapes. Acta Agriculturae Boreali-occidentalis Sinica, 17 (6):113-118. (in Chinese)
	李金月, 王飞, 王跃进, 魏艳霞. 2008. 无核葡萄与有核葡萄胚挽救杂交后代的抗寒性研究. 西北农业学报, 17 (6):113-118.
[22]	Li S, Li Z, Zhao Y, Zhao J, Luo Q, Wang Y. 2020a. New disease-resistant,seedless grapes are developed using embryo rescue and molecular markers. 3 Biotech, 10 (1):4. doi: 10.1007/s13205-019-1993-0 URL
[23]	Li S, Liu K, Yu S, Jia S, Chen S, Fu Y, Sun F, Luo Q, Wang Y. 2020b. The process of embryo abortion of stenospermocarpic grape and it develops into plantlet in vitro using embryo rescue. Plant Cell,Tissue & Organ Culture, 143 (2):389-409.
[24]	Li Sheng-chen, Wen Li-zhu, Zhang Feng-qin, Luo Fang-mei, Yang Mei-rong, Zhang Ying-zhu, Huang De-pan. 1983. The new cold and disease-resistant cultivar- Beichun. Chinese Bulletin of Botany,(2):30-32. (in Chinese)
	黎盛臣, 文丽珠, 张凤琴, 罗方梅, 杨美容, 张映祝, 黄德藩. 1983. 抗寒抗病葡萄新品种-北醇. 植物学通报,(2):30-32.
[25]	Li T, Li Z, Yin X, Guo Y, Wang Y, Xu Y. 2018. Improved in vitro Vitis vinifera L. embryo development of F₁progeny of‘Delight’×‘Ruby Seedless’ using putrescine and marker-assisted selection. In Vitro Cellular & Developmental Biology-Plant, 54 (3):291-301.
[26]	Li Z, Li T, Wang Y, Xu Y. 2015. Breeding new seedless grapes using in ovulo embryo rescue and marker-assisted selection. In Vitro Cellular & Developmental Biology-Plant, 51 (3):241-248.
[27]	Liu Chong-huai, Jiang Jian-fu, Fan Xiu-cai, Zhang Ying. 2014. The utilization of Chinese wild grape species in production and breeding. Journal of Plant Genetic Resources, 15 (4):720-727. (in Chinese)
	刘崇怀, 姜建福, 樊秀彩, 张颖. 2014. 中国野生葡萄资源在生产和育种中利用的概况. 植物遗传资源学报, 15 (4):720-727.
[28]	Liu Q, Zhang J, Wang Y, Yu D, Xia H. 2016. Breeding for cold-resistant,seedless grapes from Chinese wild Vitis amurensis using embryo rescue. New Zealand Journal of Crop and Horticultural Science, 44 (2):136-151. doi: 10.1080/01140671.2016.1153489 URL
[29]	Liu Qiao, Zhang Jian-xia, Yu Dong-dong, Wang Yue-jin. 2015. Influence of 6-BA on embryo rescue of seedless grape with cold resistance. Journal of Fruit Science, 32 (5):903-908. (in Chinese)
	刘巧, 张剑侠, 于冬冬, 王跃进. 2015. 6-BA对抗寒无核葡萄胚挽救的影响. 果树学报, 32 (5):903-908.
[30]	Liu S M, Sykes S R, Clingeleffer P R, Liu S M, Sykes S R, Clingeleffer P R. 2003. Improved in ovulo embryo culture for stenospermocarpic grapes (Vitis vinifera L.). Australian Journal of Agricultural Research, 54 (9):869-876. doi: 10.1071/AR03053 URL
[31]	Mejía N, Hinrichsen P. 2003. A new,highly assertive scar marker potentially useful to assist selection for seedlessness in table grape breeding. Acta Horticultura, 603:559-564.
[32]	Mejía N, Soto B, Guerrero M, Casanueva X, Houel C, Miccono Mde los Á, Ramos R, Cunff L L, Boursiquot J M, Hinrichsen P, Adam-Blondon A. 2011. Molecular,genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine. BMC Plant Biology, 11 (1):57. doi: 10.1186/1471-2229-11-57 URL
[33]	Niu Ru-xuan, Zhang Jiang-xia, Wang Yue-jin, Zhai Huan, Zhao Kai. 2012. Embryo rescue of seedless grape with disease and cold resistance. Journal of Fruit Science, 29 (5):825-829. (in Chinese)
	牛茹萱, 张剑侠, 王跃进, 翟焕, 赵凯. 2012. 抗病抗寒无核葡萄胚挽救研究. 果树学报, 29 (5):825-829.
[34]	Notsuka K, Tsuru T, Shiraishi M. 2001. Seedless-seedless grape hybridization via in-ovluo embryo culture. Journal of the Japanese Society for Horticultural Science, 70 (1):7-15.
[35]	Pan Xue-jun. 2005. Innovating in the technique system of embryo rescue of stenospermocarpic grape and breeding new cultivars of both seedless and disease-resistance traits[Ph.D.Dissertation]. Yangling:Northwest A & F University. (in Chinese)
	潘学军. 2005. 无核抗病葡萄胚挽救技术体系优化及新品系培育[博士论文]. 杨凌: 西北农林科技大学.
[36]	Pellerone F I, Edwards K J, Thomas M R. 2001. Grapevine microsatellite repeats:isolation,characterisation and use for genotyping of grape germplasm from Southern Italy. Vitis, 40 (4):179-186.
[37]	Pommer C V, Ramming D W, Emershad R L. 1995. Influence of grape genotype,ripening season,seed trace size,and culture date on in ovule embryo development and plant formation. Bragantia, 54 (2):237-249. doi: 10.1590/S0006-87051995000200002 URL
[38]	Ponce M T, Aguero C, Gregorimt M T, Tizio R. 2000. Factors affecting development of stenospermic grape Vitis vinifera L. embryo cultured in vitro. Acta Horticulturae, 528:667-671.
[39]	Ramming D W, Emershad R L. 1982. In ovulo embryo culture of seeded and seedless Vitis vnifera L. HortScience, 17:576.
[40]	Ramming D W, Emershad R L, Tarailn R. 2000. A stenospermocarpic,seedless Vitis vinifera × Vitis rotundifolia hybrid developed by embryo rescue. HortScience, 35 (4):732-734. doi: 10.21273/HORTSCI.35.4.732 URL
[41]	Song Run-gang, Lu Wen-peng, Guo Tai-jun, Liu Jing-kuan, Shen Yu-jie, Lin Xing-gui, Li Xiao-hong, Guo Zhen-gui. 2005. A new dry-red brewing grape variety‘Zuoyouhong’. Acta Horticulturae Sinica, 32 (4):757. (in Chinese)
	宋润刚, 路文鹏, 郭太君, 刘景宽, 沈育杰, 林兴桂, 李晓红, 郭振贵. 2005. 酿造干红葡萄酒新品种‘左优红’. 园艺学报, 32 (4):757.
[42]	Song Run-gang, Lu Wen-peng, Shen Yu-jie, Jin Ren-hao, Li Xiao-hong, Guo Zhen-gui, Liu Jing-kuan, Lin Xing-gui. 2008. A new ice-red wine grape variety-Beibinghong. Sino-Overseas Grapevine & Wine,(4):19-22. (in Chinese)
	宋润刚, 路文鹏, 沈育杰, 金仁浩, 李晓红, 郭振贵, 刘景宽, 林兴桂. 2008. 冰红葡萄酒新品种“北冰红”的选育研究. 中外葡萄与葡萄酒,(4):19-22.
[43]	Song Run-gang, Lu Wen-peng, Zhang Qing-tian, Li Xiao-hong, Yang Yi-ming, Fan Shu-tian, Ai-Jun, Shen Yu-jie, Lin Xing-gui. 2012. A new cultivar of Vitis amurensis‘Xuelanhong’for dry-red brewing. Acta Horticulturae Sinica, 39 (11):2312-2314. (in Chinese)
	宋润刚, 路文鹏, 张庆田, 李晓红, 杨义明, 范书田, 艾军, 沈育杰, 林兴桂. 2012. 酿酒葡萄新品种‘雪兰红’. 园艺学报, 39 (11):2312-2314.
[44]	Spiegel-Roy P, Sahar N, Baron J, Lavi U. 1985. In vitro culture and plant formation from grape cultivars with abortive ovules and seeds. Journal of the American Society for Horticultural Science, 110 (1):109-112.
[45]	Tian L, Wang Y. 2008. Seedless grape breeding for disease resistance by using embryo rescue. Vitis, 47 (1):15-19.
[46]	Tian L, Wang Y, Niu L, Tang D. 2008. Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp. I. in vitro embryo rescue and plant development. Scientia Horticulturae, 117 (2):136-141. doi: 10.1016/j.scienta.2008.03.024 URL
[47]	Wang Fa-wei, Liu Yang, Wu Xue-yan, Li Xiao-wei, Li Hai-yan. 2013. Cloning and expression analysis of VaCBF1 transcription factor gene in grape. Journal of Northwest A & F University(Natural Science Edition), 41 (12):86-92. (in Chinese)
	王法微, 刘洋, 吴学彦, 李晓薇, 李海燕. 2013. 山葡萄VaCBF1转录因子基因的克隆与表达分析. 西北农林科技大学学报(自然科学版), 41 (12):86-92.
[48]	Wang Y J, Lamikanra O, Lu J, Ramming D. 1996. Identification of genetic marker linked to seedless genes in grapes using RAPD. Acta University Agriculture Boreali-Occidentalis, 24 (5):12-20,21.
[49]	Wang Yue-jin, Lamikanra O. 2002. Application and synthesis on the DNA probe for detecting seedless genes in grapevine. Journal of Northwest A&F University(Natural Science Edition), 30 (3):42-44. (in Chinese)
	王跃进, Lamikanra O. 2002. 检测葡萄无核基因DNA探针的合成与应用. 西北农林科技大学学报(自然科学版), 30 (3):42-44.
[50]	Zhang C, Fan X C, Liu C H, Fang J G. 2021. Anatomical berry characteristics during the development of grape berries with different shapes Horticultural Plant Journal, 7 (4):295-306.
[51]	Zhang Hong-ming, Meng Xin-fa, Zhang Lu-sheng. 1990. A study on ovule development of seedless grapes and its early culture in vitro Ⅰ. the effect of plant growth regulators on ovule culture in vitro. Acta Agricultura Universitatis Pekinensis, 16 (3):277-283. (in Chinese)
	张宏明, 孟新法, 张潞生. 1990. 无核葡萄胚珠发育及早期离体培养的研究Ⅰ. 激素对胚珠离体培养的影响. 北京农业大学学报, 16 (3):277-283.
[52]	Zhang Jian-xia, Niu Ru-xuan. 2013. The present situation and prospect of embryo rescue technique research in seedless grape breeding. Acta Horticulturae Sinica, 40 (9):1645-1655. (in Chinese)
	张剑侠, 牛茹萱. 2013. 无核葡萄胚挽救技术的研究现状与展望. 园艺学报, 40 (9):1645-1655.
[53]	Zhang Jian-xia, Xiong Yan, Wang Yue-jin, Zhu Zi-guo. 2010. RAPD markers linked to the hardiness gene in Chinese wild Vitis and their sequence analysis. Chinese Agricultural Science Bulletin, 26 (10):30-37. (in Chinese)
	张剑侠, 熊燕, 王跃进, 朱自果. 2010. 中国野生葡萄抗寒基因的RAPD标记及其序列分析. 中国农学通报, 26 (10):30-37.
[54]	Zhao Kai. 2013. Study on the embryo rescue of seedless grape with cold resistance and the functional analysis of VaERD[M. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	赵凯. 2013. 抗寒无核葡萄胚挽救及VaERD基因功能研究[硕士论文]. 杨凌: 西北农林科技大学.
[55]	Zhao Kai, Liu Qiao, Zhang Jian-xia, Niu Ru-xuan, Li Gui-rong, Wang Yue-jin. 2013. Impacting factors on embryo rescue of seedless grape with cold resistant. Northern Horticulture,(17):11-15. (in Chinese)
	赵凯, 刘巧, 张剑侠, 牛茹萱, 李桂荣, 王跃进. 2013. 抗寒无核葡萄胚挽救影响因素的研究. 北方园艺,(17):11-15.
[56]	Zhao Ya-nan, Luo Qiang-wei, Wang Yue-jin. 2018. Breeding for grape germplasm involved in seedlessness with cold-resistant using embryo rescue. Scientia Agricultura Sinica, 51 (21):4119-4130. (in Chinese)
	赵雅楠, 骆强伟, 王跃进. 2018. 利用胚挽救技术创制无核抗寒葡萄新种质. 中国农业科学, 51 (21):4119-4130.
[57]	Zhao Ying. 2018. Evaluation for cold tolerance in germplasms of Vitis amurensis and study on mechanisms of cold tolerance[Ph. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	赵滢. 2018. 山葡萄种质抗寒性评价及抗寒机制研究[博士论文]. 北京: 中国农业科学院.
[58]	Zhu P, Gu B, Li P, Shu X, Zhang X, Zhang J. 2020. New cold-resistant,seedless grapes developed using embryo rescue and marker-assisted selection. Plant Cell,Tissue & Organ Culture, 140 (3):551-562.

亲本 Parent	名称 Name	特性 Characteristic	来源 Origin
母本 Female	火焰无核 Flame Seedless	无核、红色、早熟 Stenospermocarpic,red berries,early maturing	（Cardinal × Thompson Seedless）× [（Red Malaga × Tifafini Ahmer）×（Muscat Alexandria × Thompson Seedless）]
	昆香无核 Kunxiang Seedless	无核、金黄色、早熟、玫瑰香味 Stenospermocarpic,yellow berries,early maturing,aroma of muscat	Queen of Vineyard × 康耐诺 Queen of Vineyard × Kangnainuo
	赫什无核 Heshi Seedless	无核、绿色 Stenospermocarpic,green berries	欧亚种 Vitis. vinifera
	红无籽露 Sultanina Rose	无核、红色 Stenospermocarpic,red berries	欧亚种 V. vinifera
	红宝石无核 Ruby Seedless	无核、红色、大粒、中熟 Stenospermocarpic,red berries,big berry,mid-maturing	Emperor × Q25-6
	克瑞森无核 Crimson Seedless	无核、红色、大粒、晚熟 Stenospermocarpic,red berries,big berry,late maturing	C11-160 × G4-74
	无核紫 Black Monukka	无核、紫黑色 Stenospermocarpic,black	欧亚种 V. vinifera
	波尔莱特 Perlette	无核、中熟、绿色 Stenospermocarpic,green berries,mid-maturing	Queen of Vineyard × Scolokertek Kiralynoie
父本 Male	左优红 Zuoyouhong	有核、蓝黑色、抗寒、抗病、抗旱 Seeded,blue and black,cold-resistance,disease- resistance,drought-resistance	79-26-58 × 74-1-326
	北冰红 Beibinghong	有核、抗寒、抗病 Seeded,cold-resistance,disease-resistance	左优红 × 86-4-53 Zuoyouhong × 86-4-53
	雪兰红 Xuelanhong	有核、蓝黑色、抗寒 Seeded,blue and black,cold-resistance	左优红 × 北冰红 Zuoyouhong × Beibinghong
	北醇 Beichun	有核、紫黑色、抗寒 Seeded,black,late maturing,cold-resistance	玫瑰香 × 野生山葡萄 Muscat Hamburgh × Vitis amurensis

亲本 Parent	名称 Name	特性 Characteristic	来源 Origin
母本 Female	火焰无核 Flame Seedless	无核、红色、早熟 Stenospermocarpic,red berries,early maturing	（Cardinal × Thompson Seedless）× [（Red Malaga × Tifafini Ahmer）×（Muscat Alexandria × Thompson Seedless）]
	昆香无核 Kunxiang Seedless	无核、金黄色、早熟、玫瑰香味 Stenospermocarpic,yellow berries,early maturing,aroma of muscat	Queen of Vineyard × 康耐诺 Queen of Vineyard × Kangnainuo
	赫什无核 Heshi Seedless	无核、绿色 Stenospermocarpic,green berries	欧亚种 Vitis. vinifera
	红无籽露 Sultanina Rose	无核、红色 Stenospermocarpic,red berries	欧亚种 V. vinifera
	红宝石无核 Ruby Seedless	无核、红色、大粒、中熟 Stenospermocarpic,red berries,big berry,mid-maturing	Emperor × Q25-6
	克瑞森无核 Crimson Seedless	无核、红色、大粒、晚熟 Stenospermocarpic,red berries,big berry,late maturing	C11-160 × G4-74
	无核紫 Black Monukka	无核、紫黑色 Stenospermocarpic,black	欧亚种 V. vinifera
	波尔莱特 Perlette	无核、中熟、绿色 Stenospermocarpic,green berries,mid-maturing	Queen of Vineyard × Scolokertek Kiralynoie
父本 Male	左优红 Zuoyouhong	有核、蓝黑色、抗寒、抗病、抗旱 Seeded,blue and black,cold-resistance,disease- resistance,drought-resistance	79-26-58 × 74-1-326
	北冰红 Beibinghong	有核、抗寒、抗病 Seeded,cold-resistance,disease-resistance	左优红 × 86-4-53 Zuoyouhong × 86-4-53
	雪兰红 Xuelanhong	有核、蓝黑色、抗寒 Seeded,blue and black,cold-resistance	左优红 × 北冰红 Zuoyouhong × Beibinghong
	北醇 Beichun	有核、紫黑色、抗寒 Seeded,black,late maturing,cold-resistance	玫瑰香 × 野生山葡萄 Muscat Hamburgh × Vitis amurensis

用途 Usage	基因 Gene	引物序列（5′-3′） Primers sequence	参考文献 Reference
分子标记 Molecular marker	GSLP1	CCAGTTCGCCCGTAAATG	Wang et al.,1996;王跃进和Lamikanra,2002
	SCF27	F:CAGGTGGGAGTAGTGGAATG;R:CAGGTGGGAGTAAGATTTGT	Mejía & Hinrichsen,2003
	S241	TGACGTCCGT	张剑侠等,2010
qRT-PCR	VvCOR27	F:GAGGGTTGTGGGAGTGCG;R:AACTGTGCTTCTCTGTCCAT	范高韬,2015
	VaCBF1	F:GGAGACACGACACCCAATATAC;R:GGAAAGGTGCCTAGCCATATC	王法微等,2013
	VaERD15	F:TACAGTCCTCTCCCTGGTTT;R:GAGGGCAGGATCGTCAATATC	赵凯,2013
	VvActin	F:CTATCCTTCGTCTTGACCTTGCTG;R:AGTGGTGAACATGTAACCCCTCTC	丁兰,2019

用途 Usage	基因 Gene	引物序列（5′-3′） Primers sequence	参考文献 Reference
分子标记 Molecular marker	GSLP1	CCAGTTCGCCCGTAAATG	Wang et al.,1996;王跃进和Lamikanra,2002
	SCF27	F:CAGGTGGGAGTAGTGGAATG;R:CAGGTGGGAGTAAGATTTGT	Mejía & Hinrichsen,2003
	S241	TGACGTCCGT	张剑侠等,2010
qRT-PCR	VvCOR27	F:GAGGGTTGTGGGAGTGCG;R:AACTGTGCTTCTCTGTCCAT	范高韬,2015
	VaCBF1	F:GGAGACACGACACCCAATATAC;R:GGAAAGGTGCCTAGCCATATC	王法微等,2013
	VaERD15	F:TACAGTCCTCTCCCTGGTTT;R:GAGGGCAGGATCGTCAATATC	赵凯,2013
	VvActin	F:CTATCCTTCGTCTTGACCTTGCTG;R:AGTGGTGAACATGTAACCCCTCTC	丁兰,2019

杂交组合 Cross	果粒数 No. of berries	接种胚珠数 No. of cultured ovules	发育胚数 No. of embryos developed	胚发育率/% Per of embryo developed	成苗数 No. of plants development	成苗率/% Per of plants development
火焰无核 × 左优红 Flame Seedless × Zuoyouhong	567	831	156	18.77	49	5.90
火焰无核 × 北冰红 Flame Seedless × Beibinghong	974	1 392	251	18.03	79	5.68
昆香无核 × 左优红 Kunxiang Seedless × Zuoyouhong	812	451	114	25.28	22	4.88
昆香无核 × 雪兰红 Kunxiang Seedless × Xuelanhong	700	357	107	29.97	32	8.96
昆香无核 × 北醇 Kunxiang Seedless × Beichun	651	455	128	28.13	39	8.57
赫什无核 × 北冰红 Heshi Seedless × Beibinghong	424	273	43	15.75	12	4.40
赫什无核 × 雪兰红 Heshi Seedless × Xuelanhong	611	485	82	16.91	23	4.74
红无籽露 × 雪兰红 Sultanina Rose × Xuelanhong	743	1 106	10	0.90	3	0.27
红宝石无核 × 北冰红 Ruby Seedless × Beibinghong	1 870	3 497	1 559	44.58	634	18.13
红宝石无核 × 雪兰红 Ruby Seedless × Xuelanhong	320	446	224	50.22	51	11.43
克瑞森无核 × 北冰红 Crimson Seedless × Beibinghong	931	791	26	3.29	6	0.76
无核紫 × 北冰红 Black Monukka × Beibinghong	735	569	33	5.80	7	1.23
波尔莱特 × 北醇 Perlette × Beichun	1 030	1 029	226	21.96	61	5.93
红无籽露 × 红宝石无核Sultanina Rose × Ruby Seedless	1 264	1 056	21	1.99	3	0.28

The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes

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