Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 723-738.doi: 10.16420/j.issn.0513-353x.2021-0141

• Research Papers • Previous Articles     Next Articles

The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes

LI Shasha1, YU Saisai1, FU Yuheng1, LUO Qiangwei2, XU Yan1, WANG Yuejin1,**()   

  1. 1. State Key Laboratory of Crop Stress Biology in Arid Areas,Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest,Ministry of Agriculture and Rural Affairs,College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100,China
    2. Institute of Grapes and Melons on the Research in Xinjiang, Shanshan,Xinjiang 838200,China
  • Received:2021-11-15 Revised:2022-01-18 Online:2022-04-25 Published:2022-04-24
  • Contact: WANG Yuejin


New germplasms of seedless and cold-resistant grapes were cultivated by embryo rescue techniques after crossing in the field and by molecular marker-assisted selection using stenospermocarpic seedless grapes as the female parents and varieties of Chinese cold-resistant wild species or hybrids from Vitis vinifera × V. amurensis as the male parents. Among the 14 hybrid combinations,a total of 1 021 hybrid plantlets were obtained,among which 634 plantlets were obtained in the combination of‘Ruby Seedless’בBeibinghong’with the highest seedling rate of 18.13%. When‘Zuoyouhong’was the male parent,‘Flame Seedless’was suitable as female parent. When‘Beibinghong’and‘Xuelanhong’were as the male parent,‘Ruby Seedless’was suitable as female parent. When‘Beichun’as the male parent,‘Kunxiang Seedless’was suitable as female parent. A total of 132 lines related to seedlessness preliminary screened by the two seedless molecular markers SCF27 and GSLP1. A total of 190 lines related to cold-resistant were initially screened through the molecular marker S241-717. Among these hybrid progeny screened,59 lines possessed both seedless and cold-resistant traits. The grapevine cold-related genes CBF1,COR27 and ERD15 detected by qRT-PCR in the lines with or without cold-resistant traits of the hybrid progeny of‘Ruby Seedless’בBeibinghong’and‘Ruby Seedless’בXuelanhong’under cold treatment were differential expression,and the cold-related lines showed significant expression of the cold-resistant genes.

Key words: grape, seedlessness, cold-resistance, embryo rescue, molecular marker

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