%0 Journal Article %A Yan HE %A Yanli SUN %A Fangfang ZHAO %A Hongjun DAI %T Effect of Exogenous Brassinolides Treatment on Sugar Metabolism of Merlot Grape Berries %D 2022 %R 10.16420/j.issn.0513-353x.2020-0834 %J Acta Horticulturae Sinica %P 117-128 %V 49 %N 1 %X

The purpose of this study was to explore the effect of exogenous brassinolides(2,4- Epibrassinolide,EBR)on sugar metabolism of Merlot grape berries. 10-year-old grape Vitis vinifera ‘Merlot’was used as the test material,and different concentrations of EBR(0.2,0.4,0.6 mg · L-1)solutions were sprayed in the eighth week after anthesis,with distilled water as the control. Using chemical analysis,HPLC,Enzyme-linked Immunosorbent assay,and transcription analysis to study the effects of EBR treatment on the sugar components,key sugar metabolism enzymes and related gene expression during the ripening process of‘Merlot’grapes. Compared with the control,0.6 mg · L-1 EBR treatment can significantly promote the accumulation of glucose and fructose in grape berries,the contents were respectively increased by 16.95% and 39.31%. At maturation,the activities of acid invertase(AI)and sucrose phosphate synthase(SPS)were significantly higher than the control. The 0.6 mg · L-1 EBR significantly up-regulated the transcription levels of cell wall acid invertase(VvcwINV),sucrose transporter(VvSUC12),and sucrose synthetase(VvSS)during the process of grape color transformation to maturity. The expression of VvSS gene was 2.75 times that of the control. Correlation analysis showed that hexose was significantly positively correlated with SS and AI under 0.6 mg · L-1 EBR treatment. 0.6 mg · L-1 EBR treatment improves the activity of SS and AI,and promotes the expression of VvSS,VvcwINV gene and sucrose transporter VvSUC12 gene,thereby increasing the content of glucose and fructose. 0.6 mg · L-1 EBR treatment has the best effect.

%U https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0834