Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 995-1007.doi: 10.16420/j.issn.0513-353x.2021-0533

• Research Papers • Previous Articles     Next Articles

Genome-wide Identification of Grape GRF Family and Expression Analysis

LIANG Chen, SUN Ruyi, XIANG Rui, SUN Yimeng, SHI Xiaoxin, DU Guoqiang(), WANG Li()   

  1. College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China
  • Received:2022-01-27 Revised:2022-03-28 Online:2022-05-25 Published:2022-05-25
  • Contact: DU Guoqiang,WANG Li;


The gene structure analysis,protein conserved motif identification,subcellular localization prediction,synteny analysis,phylogenetic analysis,and cis-acting elements analysis of Growth-regulating factor(GRF)family members were performed. Expression patterns of grape GRF genes in different plant parts and against different hormone applications were analyzed. Further expression patterns were compared during different stages of seed development in seeded and seedless cultivars. Eight grape GRF genes were identified and divided into four groups(A-D). Seven of the genes were unevenly distributed on six chromosomes having and the number of amino acids was 213-604. All grape GRF proteins were predicted in the nucleus. Moreover,VvGRFs in the same clade showed conserved exon-intron structure and motif distribution patterns. All GRF proteins contained QLQ and WRC domains in the N-terminal region and at least one TQL or FFD domain in the C-terminal region. Synteny analysis showed that VvGRF3 and VvGRF4 were segmentally duplicated. Various cis-acting elements related to growth and development,hormones response,and stresses were found in the promoter region of VvGRF genes. Expression analysis illustrated that most of the VvGRFs were highly expressed in vegetative organs i.e. leaves. However,VvGRF2 was highly expressed in reproductive organs like flowers and fruits. The expression of VvGRF3 and VvGRF6 was significantly higher during seed development in seedless grapes as compared to seeded grapes,while VvGRF8 showed high expression in seeded grapes. The expression of VvGRF genes was induced against GA3 and IAA treatments,and most of the VvGRFs were down-regulated at 0.5 or 1 h.

Key words: grape, GRF, family evolution, seed development, GA3, IAA, expression analysis

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