Genome-wide Identification and Expression Analysis of CsTIFY Transcription Factor Family Under Abiotic Stress and Hormone Treatments in Camellia sinensis

doi:10.16420/j.issn.0513-353x.2020-0850

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 100-116.doi: 10.16420/j.issn.0513-353x.2020-0850

• Research Papers • Previous Articles Next Articles

Genome-wide Identification and Expression Analysis of CsTIFY Transcription Factor Family Under Abiotic Stress and Hormone Treatments in Camellia sinensis

XIE Siyi¹^,³, ZHOU Chengzhe¹^,²^,³, ZHU Chen¹^,²^,³, ZHAN Dongmei¹^,³, CHEN Lan¹^,³, WU Zuchun¹^,³, LAI Zhongxiong¹^,², GUO Yuqiong¹^,³^,^*()

¹College of Horticulture,Tea Industry Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China
²Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
³Key Laboratory of Tea Science of Fujian Province,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Received:2021-04-23 Revised:2021-07-26 Online:2022-01-25 Published:2022-01-24
Contact: GUO Yuqiong E-mail:guoyq828@163.com

Abstract

Abstract:

TIFY transcription factors are unique transcription factors of terrestrial plants. They are divided into four sub-families TIFY,PPD,JAZ,ZML according to structural domain characteristics,and regulate the growth and development of plants. In this paper,the TIFY transcription factor of tea plant is the research object. A total of 22 members of the CsTIFY transcription factor family have been identified from the tea plant genome database,all of which contain the TIFY domain. Gene structure analysis found that most of the CsTIFY family members have exons between five and 12,and the same subfamily contains similar numbers of motifs and exons. The results of the phylogenetic tree showed that the 122 genes of six species were divided into four groups,among which tea trees were more closely related to grapes and poplars. Analysis of promoter cis-acting elements found that the CsTIFY family contains a large number of phytohormones and abiotic stress response elements such as SA,ABA,low temperature and drought. The results of subcellular localization experiments showed that CsTIFY1,CsJAZ1 and CsJAZ5 were localized in the nucleus. The tissue expression analysis of different tea cultivars showed that most CsTIFY gene family members had higher relative expression levels in mature leaves,roots and fruits. In addition,the expression of most CsJAZ genes was increased and the expression of CsZML was decreased under the treatments of exogenous hormones ABA,SA and MeJA. The expression of CsZML1 was significantly increased under both low temperature and drought treatments.

Key words: Camellia sinensis, TIFY transcription factor, genome-wide identification, ABA, SA, MeJA, abiotic stress, subcellular localization

CLC Number:

S571.1

XIE Siyi, ZHOU Chengzhe, ZHU Chen, ZHAN Dongmei, CHEN Lan, WU Zuchun, LAI Zhongxiong, GUO Yuqiong. Genome-wide Identification and Expression Analysis of CsTIFY Transcription Factor Family Under Abiotic Stress and Hormone Treatments in Camellia sinensis[J]. Acta Horticulturae Sinica, 2022, 49(1): 100-116.

Figures/Tables 9

References 42

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用途 Purpose	基因 Gene	上游引物序列（5′-3′） Forward primer	下游引物序列（5′-3′） Reverse primer
qRT-PCR	CsTIFY1	TGAGTAACCGATTCGCTGG	GCTTGTGGAAACCCATCTCTG
	CsTIFY2	TCAACGAGGAAGAGGCTATGG	GGGTCCTCATCCTCAACCATT
	CsTIFY3	GCCTCTGTTGGTGGTTCTTC	CGGTTATTCATCTCAGTCCCT
	CsTIFY4	TCTCCGCCACTTCTGATCTG	GCGAATCGGTTACTCATCTCAG
	CsPPD1	CCTACCAGATGTGCGAGAATG	TCGCCTTCATCCAAGCTGAT
	CsPPD2	GCAAATTCTCCATTGCTCGC	TCCACGACGGTCTTCTCATC
	CsJAZ1	TCCTCCAAACCTCTTCCCAAT	CACAGAGCCAGAAGCAATCAC
	CsJAZ2	TCATTGATCGCCGAAGAAGC	GAATAGAGAGGCAAAGCAGG
	CsJAZ4	TGACCCACTTGCTGCTTCT	GAACGCCACCTTGTCCAT
	CsJAZ5	TGTCGAGTTCGTCTGGTTCT	CCGAATGTGCCTTTCTTCTC
	CsJAZ6	CGGTGGCTGTCAAAGAAGGTATT	CTCTAATCGCCATTTCAGTCCCT
	CsJAZ7	TCATTGATCGCCGAAGAAGC	GAATAGAGAGGCAAAGCAGGAG
	CsJAZ8	CGGATTACAACCACCACCAC	TCGTTAGCGGCTGTTCCTGT
	CsZML1	GGAGGGAGGAATCTTTGTTTGG	CTGCCAGTTTCTCCTGTTACAAC
	CsZML2	CTAAACGTTCCCAACCACTTCAC	GTCGTTGAGAGAGTGAGTTTCGT
	CsZML3	TGCAGCACATGAGAAATGG	GAACACATAAACCTGACCCTGG
	CsZML4	CCAGAGACGAATCACCAATCC	CTCACCACCCACAACTTCATC
	CsZML5	CCGATTCCACTTCAAGCAAG	TCCTCCTCCTCCGTCTTCAT
	CsZML6	TCGATGAAGACGGAGGAGG	GGGTTGTTATTCACAGGGAGG
	CsZML7	CGAATCCACAGCCACTTCAAG	TCCGCCTCATCCATAGCATC
	CsZML8	CTGAGACCAACCACGAAGCT	TCGAATCGGATCTGAGGGTT
内参基因	β-ACTNI	GCCATCTTTGATTGGAATGG	GGTGCCACAACCTTGATCTT
Reference gene	SAND	CCAATTGCCCCCTTAATGAC	GCAATCATTTCCTTCGTGGAG
亚细胞定位	CsTIFY1-1302	ACTCTTGACCATGGTAGATCTATGATAGATAGACAGGTTGCAAATCAT	ATCTGATCCACCTTTACTAGTCTAAAAAATTGCCTTGTTCGGG
Subcellular
localization	CsJAZ1-1302	ACTCTTGACCATGGTAGATCTATGGCATCGAGATCAGCTGTTG	ATCTGATCCACCTTTACTAGTCTAATCTTCATATCTTGATGTATTCTTCCC
	CsJAZ5-1302	ACTCTTGACCATGGTAGATCTATGTCGAGTTCGTCTGGTTCTGC	ATCTGATCCACCTTTACTAGTCTACAAATGGTGCTCAAACTGCA

用途 Purpose	基因 Gene	上游引物序列（5′-3′） Forward primer	下游引物序列（5′-3′） Reverse primer
qRT-PCR	CsTIFY1	TGAGTAACCGATTCGCTGG	GCTTGTGGAAACCCATCTCTG
	CsTIFY2	TCAACGAGGAAGAGGCTATGG	GGGTCCTCATCCTCAACCATT
	CsTIFY3	GCCTCTGTTGGTGGTTCTTC	CGGTTATTCATCTCAGTCCCT
	CsTIFY4	TCTCCGCCACTTCTGATCTG	GCGAATCGGTTACTCATCTCAG
	CsPPD1	CCTACCAGATGTGCGAGAATG	TCGCCTTCATCCAAGCTGAT
	CsPPD2	GCAAATTCTCCATTGCTCGC	TCCACGACGGTCTTCTCATC
	CsJAZ1	TCCTCCAAACCTCTTCCCAAT	CACAGAGCCAGAAGCAATCAC
	CsJAZ2	TCATTGATCGCCGAAGAAGC	GAATAGAGAGGCAAAGCAGG
	CsJAZ4	TGACCCACTTGCTGCTTCT	GAACGCCACCTTGTCCAT
	CsJAZ5	TGTCGAGTTCGTCTGGTTCT	CCGAATGTGCCTTTCTTCTC
	CsJAZ6	CGGTGGCTGTCAAAGAAGGTATT	CTCTAATCGCCATTTCAGTCCCT
	CsJAZ7	TCATTGATCGCCGAAGAAGC	GAATAGAGAGGCAAAGCAGGAG
	CsJAZ8	CGGATTACAACCACCACCAC	TCGTTAGCGGCTGTTCCTGT
	CsZML1	GGAGGGAGGAATCTTTGTTTGG	CTGCCAGTTTCTCCTGTTACAAC
	CsZML2	CTAAACGTTCCCAACCACTTCAC	GTCGTTGAGAGAGTGAGTTTCGT
	CsZML3	TGCAGCACATGAGAAATGG	GAACACATAAACCTGACCCTGG
	CsZML4	CCAGAGACGAATCACCAATCC	CTCACCACCCACAACTTCATC
	CsZML5	CCGATTCCACTTCAAGCAAG	TCCTCCTCCTCCGTCTTCAT
	CsZML6	TCGATGAAGACGGAGGAGG	GGGTTGTTATTCACAGGGAGG
	CsZML7	CGAATCCACAGCCACTTCAAG	TCCGCCTCATCCATAGCATC
	CsZML8	CTGAGACCAACCACGAAGCT	TCGAATCGGATCTGAGGGTT
内参基因	β-ACTNI	GCCATCTTTGATTGGAATGG	GGTGCCACAACCTTGATCTT
Reference gene	SAND	CCAATTGCCCCCTTAATGAC	GCAATCATTTCCTTCGTGGAG
亚细胞定位	CsTIFY1-1302	ACTCTTGACCATGGTAGATCTATGATAGATAGACAGGTTGCAAATCAT	ATCTGATCCACCTTTACTAGTCTAAAAAATTGCCTTGTTCGGG
Subcellular
localization	CsJAZ1-1302	ACTCTTGACCATGGTAGATCTATGGCATCGAGATCAGCTGTTG	ATCTGATCCACCTTTACTAGTCTAATCTTCATATCTTGATGTATTCTTCCC
	CsJAZ5-1302	ACTCTTGACCATGGTAGATCTATGTCGAGTTCGTCTGGTTCTGC	ATCTGATCCACCTTTACTAGTCTACAAATGGTGCTCAAACTGCA

基因名称 Gene name	ID	氨基酸 Amino acid	分子量/kD Molecular weight	等电点 Isoelectric point	内含子 Intron	外显子 Exons	不稳定指数 Instability index	脂肪族指数 Aliphatic index	平均疏水性 GRAVY	亚细胞定位 Subcellular localization
CsTIFY1	TEA007552.1	250	26.77771	9.90	1	2	52.44	55.04	-0.694	细胞核
										Cell nucleus
CsTTFY2	TEA012041.1	934	103.1778	5.57	11	12	49.30	84.03	-0.140	叶绿体
										Chloroplast
CsTIFY3	TEA032457.1	352	38.90351	10.25	4	5	53.41	72.81	-0.461	液泡 Vacuole
CsTIFY4	TEA009327.1	393	41.65471	9.40	5	6	46.36	65.29	-0.437	细胞核
										Cell nucleus
CsPPD1	TEA005947.1	1 820	204.7527	6.14	16	17	41.51	92.61	-0.120	胞间连丝
										Plasmodesma
CsPPD2	TEA011549.1	1 114	125.3216	8.23	12	13	50.60	88.93	-0.299	细胞核
										Cell nucleus
CsJAZ1	TEA027049.1	212	23.87791	9.15	5	6	55.42	67.08	-0.632	细胞核
										Cell nucleus
CsJAZ2	TEA030826.1	148	16.39844	7.84	0	1	48.12	58.65	-0.564	细胞核
										Cell nucleus
CsJAZ3	TEA001501.1	261	29.26615	9.17	4	5	57.65	70.69	-0.666	细胞核
										Cell nucleus
CsJAZ4	TEA001681.1	410	42.84663	9.34	7	8	35.36	66.44	-0.310	细胞核
										Cell nucleus
CsJAZ5	TEA030190.1	294	31.53145	9.46	4	5	55.85	70.37	-0.395	细胞核
										Cell nucleus
CsJAZ6	TEA001821.1	436	46.20707	9.58	7	8	48.77	71.40	-0.343	细胞质 Cytoplasm
CsJAZ7	TEA032228.1	220	24.45353	9.18	5	6	55.48	67.36	-0.557	细胞核
										Cell nucleus
CsJAZ8	TEA004474.1	136	15.60498	9.93	2	3	84.54	75.29	-0.721	细胞核
										Cell nucleus
CsZML1	TEA033832.1	385	42.00050	4.88	10	11	42.88	70.08	-0.625	细胞核
										Cell nucleus
CsZML2	TEA033836.1	271	30.51872	4.57	4	5	46.48	78.04	-0.601	细胞核
										Cell nucleus
CsZML3	TEA013465.1	303	33.49678	6.19	6	7	31.87	60.79	-0.916	细胞核
										Cell nucleus
CsZML4	TEA014550.1	234	25.20185	5.00	3	4	50.61	71.24	-0.550	细胞核
										Cell nucleus
CsZML5	TEA014541.1	352	38.21245	4.85	10	11	50.11	72.30	-0.574	细胞核
										Cell nucleus
CsZML6	TEA011504.1	352	38.21245	4.85	10	11	50.11	72.30	-0.574	细胞核
										Cell nucleus
CsZML7	TEA002032.1	383	41.64484	4.76	10	11	54.89	63.92	-0.795	细胞核
										Cell nucleus
CsZML8	TEA001414.1	461	49.38947	5.75	11	12	41.39	73.19	-0.375	细胞核
										Cell nucleus

基因名称 Gene name	ID	氨基酸 Amino acid	分子量/kD Molecular weight	等电点 Isoelectric point	内含子 Intron	外显子 Exons	不稳定指数 Instability index	脂肪族指数 Aliphatic index	平均疏水性 GRAVY	亚细胞定位 Subcellular localization
CsTIFY1	TEA007552.1	250	26.77771	9.90	1	2	52.44	55.04	-0.694	细胞核
										Cell nucleus
CsTTFY2	TEA012041.1	934	103.1778	5.57	11	12	49.30	84.03	-0.140	叶绿体
										Chloroplast
CsTIFY3	TEA032457.1	352	38.90351	10.25	4	5	53.41	72.81	-0.461	液泡 Vacuole
CsTIFY4	TEA009327.1	393	41.65471	9.40	5	6	46.36	65.29	-0.437	细胞核
										Cell nucleus
CsPPD1	TEA005947.1	1 820	204.7527	6.14	16	17	41.51	92.61	-0.120	胞间连丝
										Plasmodesma
CsPPD2	TEA011549.1	1 114	125.3216	8.23	12	13	50.60	88.93	-0.299	细胞核
										Cell nucleus
CsJAZ1	TEA027049.1	212	23.87791	9.15	5	6	55.42	67.08	-0.632	细胞核
										Cell nucleus
CsJAZ2	TEA030826.1	148	16.39844	7.84	0	1	48.12	58.65	-0.564	细胞核
										Cell nucleus
CsJAZ3	TEA001501.1	261	29.26615	9.17	4	5	57.65	70.69	-0.666	细胞核
										Cell nucleus
CsJAZ4	TEA001681.1	410	42.84663	9.34	7	8	35.36	66.44	-0.310	细胞核
										Cell nucleus
CsJAZ5	TEA030190.1	294	31.53145	9.46	4	5	55.85	70.37	-0.395	细胞核
										Cell nucleus
CsJAZ6	TEA001821.1	436	46.20707	9.58	7	8	48.77	71.40	-0.343	细胞质 Cytoplasm
CsJAZ7	TEA032228.1	220	24.45353	9.18	5	6	55.48	67.36	-0.557	细胞核
										Cell nucleus
CsJAZ8	TEA004474.1	136	15.60498	9.93	2	3	84.54	75.29	-0.721	细胞核
										Cell nucleus
CsZML1	TEA033832.1	385	42.00050	4.88	10	11	42.88	70.08	-0.625	细胞核
										Cell nucleus
CsZML2	TEA033836.1	271	30.51872	4.57	4	5	46.48	78.04	-0.601	细胞核
										Cell nucleus
CsZML3	TEA013465.1	303	33.49678	6.19	6	7	31.87	60.79	-0.916	细胞核
										Cell nucleus
CsZML4	TEA014550.1	234	25.20185	5.00	3	4	50.61	71.24	-0.550	细胞核
										Cell nucleus
CsZML5	TEA014541.1	352	38.21245	4.85	10	11	50.11	72.30	-0.574	细胞核
										Cell nucleus
CsZML6	TEA011504.1	352	38.21245	4.85	10	11	50.11	72.30	-0.574	细胞核
										Cell nucleus
CsZML7	TEA002032.1	383	41.64484	4.76	10	11	54.89	63.92	-0.795	细胞核
										Cell nucleus
CsZML8	TEA001414.1	461	49.38947	5.75	11	12	41.39	73.19	-0.375	细胞核
										Cell nucleus

Genome-wide Identification and Expression Analysis of CsTIFY Transcription Factor Family Under Abiotic Stress and Hormone Treatments in Camellia sinensis

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