Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 622-632.doi: 10.16420/j.issn.0513-353x.2021-0267

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Construction of DNA Fingerprinting and Clustering Analysis with SSR Markers for the Muskmelon of Xizhoumi Series

LI Chao, YANG Ying, CHEN Wei, ZHENG Heyun, LIAO Xinfu, SUN Yuping*()   

  1. Research Institute of Grape and Melon of Xinjiang Uighur Autonomous Region,Shanshan,Xinjiang 838200,China
  • Received:2021-04-28 Revised:2022-01-03 Online:2022-03-25 Published:2022-03-25
  • Contact: SUN Yuping


In order to identify the cultivars quickly and accurately,DNA fingerprinting of 18 muskmelon cultivars of Xizhoumi series was constructed by using SSR marker technology. Using 18 cultivars(lines),highly informative and polymorphic simple sequence repeat(SSR)primers were screened. Out of the 73 SSR primer pairs,nine pairs including SSR12083,MU5554-1,TJ10,PM3,MU5499,SSR04219,SSR04632,SSR22874 and C30 were selected,and they could generate stable,clearly and codominant bands. Each primer pair could generate 3-6 polymorphic bands with an average of 4.33. The average polymorphism information content(PIC)was 0.45 with a range from 0.20 to 0.78. Five selected primer pairs(C30,MU5499,MU5554-1,SSR04219 and SSR12083)had established the fingerprinting of the 18 accessions and their unique fingerprint. We found that the female parent of ‘Xizhoumi 25’was the same as the female parent of‘Xizhoumi 17’. Clustering analysis showed that the similarity coefficient of the 18 accessions ranged from 0.70 to 1.00. They can be divided into three groups at 0.74. Above results indicated that the DNA fingerprinting could provide scientific basis for identification of Xizhoumi muskmelon.

Key words: muskmelon, SSR, fingerprinting, clustering analysis

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