Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool

doi:10.16420/j.issn.0513-353x.2021-0018

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 613-621.doi: 10.16420/j.issn.0513-353x.2021-0018

• Research Notes • Previous Articles Next Articles

Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool

QIAO Jun, LIU Jing, LI Suwen, WANG Liying^*()

State Key Laboratory of Vegetable Germplasm Innovation,Tianjin Enterprise Key Laboratory of Vegetable Genetics & Breeding,Tianjin Kernel Vegetable Research Institute,Tianjin 300381,China

Received:2021-08-02 Revised:2021-11-25 Online:2022-03-25 Published:2022-03-25
Contact: WANG Liying E-mail:sun_hwg@163.com

Abstract

Abstract:

Stably genetic purple under the calyx,round eggplant inbred line Y5 was crossed with green under the calyx,round eggplant inbred line Y73. The segregation of fruit color under the calyx of F₂ progeny had a normal distribution,which could be analyzed by bulk segregant analysis（BSA）. In the F₂ population,30 purple and 30 green under the calyx plants were selected to construct separated mixed pools. Whole-genome resequencing of 30× and 10× coverage was carried out in the mixed pools and parents to locate the correlation interval of fruit color under the calyx. Candidate genes were predicted according to the collinear region and gene annotation information of the eggplant genome. A total of 7 747 547 single nucleotide polymorphisms（SNP）and 569 796 insertion deletion markers（InDel）were obtained from the hybrid pools and parents,which were used for genome-wide mapping of fruit color traits under the calyx. According to pathway enrichment and gene function annotation,candidate genes EGP21397,EGP21400,EGP21491,EGP22288,and EGP22289 were obtained. The five candidate genes were closely related to cell photosynthesis and encoded calmodulin-binding family proteins,the C2H2 zinc finger protein,carotenoid isomerase,2Fe-2S ferredoxin-like superfamily proteins,and the lipid transfer protein. At the same time,six candidate genes related to cytochrome P450 and phytochrome synthesis were predicted,which were located in the association regions of chromosomes 10. These proteins and pigments were related to photosynthesis and stress development,which revealed the molecular mechanism of fruit color in eggplant.

Key words: eggplant, fruit color under the calyx, gene mapping, extreme mixing pool, whole-genome resequencing

CLC Number:

S641.1

QIAO Jun, LIU Jing, LI Suwen, WANG Liying. Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool[J]. Acta Horticulturae Sinica, 2022, 49(3): 613-621.

Figures/Tables 4

References 35

[1]	Barchi L, Lanteri S, Portis E, Valè G, Volante A, Pulcini L, Ciriaci T, Acciarri N, Barbierato V, Toppino L, Rotino G L. 2012. A RAD tag derived marker based eggplant linkage map and the location of QTLs determining anthocyanin pigmentation. PLoS ONE, 7(8):e43740.
[2]	Cericola F, Portis E, Lanteri S, Toppino L, Barchi L, Acciarri N, Pulcini L, Sala T, Rotino G L. 2014. Linkage disequilibrium and genome-wide association analysis for anthocyanin pigmentation and fruit color in eggplant. BMC Genomics, 15(1):896. doi: 10.1186/1471-2164-15-896 URL
[3]	Chen Chunguang. 2013. Study on the function of carotenoid isomerase in the operation of photosynthetic apparatus for the eggplant genome database provided[Ph. D. Dissertation]. Beijing: University of Chinese Academy of Sciences. (in Chinese)
	陈春光. 2013. 类胡萝卜素异构酶在光合机构运转中的功能研究[博士论文]. 北京: 中国科学院大学.
[4]	Chen Huoying, Liu Xinyu, Zhang Guogang, Ge Haiyan, Han Hongqiang, Zhou Tengxia, Gao Lijie, Jiang Mingmin. 2014. Genes related to anthocyanin synthesis in eggplant and their coding proteins. National invention patent. Publication No.CN104059926A. (in Chinese)
	陈火英, 刘新宇, 张国刚, 葛海燕, 韩洪强, 周腾夏, 高莉洁, 蒋明敏. 2014. 茄子花青素合成相关基因及其编码蛋白. 国家发明专利.公开号CN104059926A.
[5]	Chen Xueping. 2015. Study on genetic diversity and genetic linkage map construction in eggplant[Ph. D. Dissertation]. Baoding: Hebei Agricultural University. (in Chinese)
	陈雪平. 2015. 茄子遗传多样性研究与遗传连锁图谱构建[博士论文]. 保定: 河北农业大学.
[6]	Docimo T, Francese G, Ruggiero A, Batelli G, de Palma M, Bassolino L, Toppino L, Rotino G L, Mennella G, Tucci M. 2016. Phenylpropanoids accumulation in eggplant fruit:characterization of biosynthetic genes and regulation by a MYB transcription factor. Frontiers in Plant Science, 6:1233.
[7]	Ge H, Liu Y, Zhang J, Han H, Li H, Shao W, Chen H. 2013. Simple sequence repeat-based association analysis of fruit traits in eggplant(Solanum melongena L.). Genetics & Molecular Research, 12(4):5651-5663.
[8]	Huang Ji, Wang Jianfei, Zhang Hongsheng. 2004. Structure and function of plant C2H2 zinc finger protein. Genetics, 26(3):414-418. (in Chinese)
	黄骥, 王建飞, 张红生. 2004. 植物C2H2型锌指蛋白的结构与功能. 遗传, 26(3):414-418.
[9]	Jiang M, Liu Y, Ren L, Lian H, Chen H. 2016a. Molecular cloning and characterization of anthocyanin biosynthesis genes in eggplant(Solanum melongena L.). Acta Physiologiae Plantarum, 38(7):1-13. doi: 10.1007/s11738-015-2023-4 URL
[10]	Jiang M, Ren L, Lian H, Liu Y, Chen H. 2016b. Novel insight into the mechanism underlying light-controlled anthocyanin accumulation in eggplant (Solanum melongena L.). Plant Science, 249:46-58. doi: 10.1016/j.plantsci.2016.04.001 URL
[11]	Li Changsheng, Zhao Chuanzhi, Li Aiqin, Li Xingpu, Zhao Guangjing, Wang Xingjun. 2009. Studies on plant lipid transporters. Shandong Agricultural Sciences, (8):13-17. (in Chinese)
	李长生, 赵传志, 李爱芹, 李兴圃, 赵光敬, 王兴军. 2009. 植物脂质转运蛋白的研究. 山东农业科学, (8):13-17.
[12]	Li D D, Qian J, Li W J, Jiang Y Q, Gan G Y, Li W L, Chen R Y, Yu N, Li Y, Wu Y G, Kang D X, Lian J M, Niu Y C, Wang Y K. 2019. Genome sequence and analysis of the eggplant(Solanum melongena L.). bioRxiv,doi.org/10.1101/824540. doi: doi.org/10.1101/824540
[13]	Li Huaizhi. 2011 Construction of genetic linkage map and QTL mapping of fruit related traits in eggplant[Ph. D. Dissertation]. Shanghai Jiao Tong University. (in Chinese)
	李怀志. 2011. 茄子遗传连锁图谱构建及果实相关性状QTL定位[博士论文]. 上海: 上海交通大学.
[14]	Li Qingwei, Zhang Han, Pang Yue. 2017. Research progress of calmodulin structure,properties and cell biological function. Journal of Liaoning Normal University(Natural Science Edition), 40(1):74-82. (in Chinese)
	李庆伟, 张撼, 逄越. 2017. 钙调蛋白结构、性质及其细胞生物学功能的研究进展. 辽宁师范大学学报(自然科学版), 40(1):74-82.
[15]	Li J, He Y J, Zhou L, Liu Y, Jiang M, Ren L, Chen H. 2018. Transcriptome profiling of genes related to light-induced anthocyanin biosynthesis in eggplant(Solanum melongena L.)before purple color becomes evident. BMC Genomics, 19:201. doi: 10.1186/s12864-018-4587-z URL
[16]	Li J, Ren L, Gao Z, Jiang M, Zhou L, He Y, Chen H. 2017. Combined transcriptomic and proteomic analysis constructs a new model for light-induced anthocyanin biosynthesis in eggplant(Solanum melongena L.). Plant, Cell & Environment, 40(12):3069-3087.
[17]	Li Xi-xiang, Zhu De-wei. 2006. Descriptors and data standard for eggplant(Solanum melongena L.). Beijing: China Agricultural Press. (in Chinese)
	李锡香, 朱德蔚. 2006. 茄子种质资源描述规范和数据标准. 北京: 中国农业出版社.
[18]	Li Xiang. 2011. Cloning and expression characterization of the anthocyanin biosynthesis key genes in eggplant[M. D. Dissertation]. Shanghai: Shanghai Jiao Tong University. (in Chinese)
	李翔. 2011. 茄子花青素生物合成关键基因的克隆与表达分析[硕士论文]. 上海: 上海交通大学.
[19]	Lian Yong, Liu Fuzhong, Tian Shibing, Chen Yuhui, Zhang Ying. 2017. Research progress of eggplant genetic breeding during the 12th Five Year Plan. China Vegetable, (2):14-22. (in Chinese)
	连勇, 刘富中, 田时炳, 陈钰辉, 张映. 2017. “十二五”我国茄子遗传育种研究进展. 中国蔬菜, (2):14-22.
[20]	Liu Bing, Wei Haixuan, Su Jianbin, Zhang Yang, Wang Jingfa, Wang Hongbin, Feng Dongru. 2013. Preliminary study on the effect of ferriredoxin gene deletion on flowering advance in Arabidopsis thaliana. Journal of Sun Yat sen University(Natural Science Edition), 53(3):112-116,127. (in Chinese)
	刘兵, 魏海轩, 苏建斌, 张洋, 王金发, 王宏斌, 冯冬茹. 2013. 拟南芥铁氧还蛋白基因缺失促进花期提前的初步研究. 中山大学学报(自然科学版), 53(3):112-116,127.
[21]	Liu Wei, Liu Yang, Ren Li. 2017. Effect of light on the anthocyanin synthesis and expression related genes of non-photosensitive type eggplant. Molecular Plant Breeding, 15(3):74-83. (in Chinese)
	刘卫, 刘杨, 任丽. 2017. 光照对非光敏型茄子花青素合成相关基因的影响. 分子植物育种, 15(3):74-83.
[22]	Liu Xinyu, Han Hongqiang, Ge Haiyan, Jiang Mingmin, Chen Huoying. 2014. Cloning,expression and interaction of anthocyanin-related transcription factors SmTTG1,SmGL3 and SmTT8 in eggplant. Acta Horticulturae Sinica, 41(11):2241-2249. (in Chinese)
	刘新宇, 韩洪强, 葛海燕, 蒋明敏, 陈火英. 2014. 茄子花青素合成中SmTTG1、SmGL3和SmTT8的表达及其蛋白质间的相互作用. 园艺学报, 41(11):2241-2249.
[23]	Nunome T, Ishigro K, Yoshida T, Hirai M. 2001. Mapping of fruit shape and color development traits in eggplant(Solanum melongena L.)based on RAPD and AFLP markers. Breeding Science, 51(1):19-26. doi: 10.1270/jsbbs.51.19 URL
[24]	Pan Ruichi. 1979. Plant physiology. Beijing: Higher Education Press. (in Chinese)
	潘瑞炽. 1979. 植物生理学. 北京: 高等教育出版社.
[25]	Shao Wenting. 2013. Cloning and functional analysis of SmMYB gene related to anthocyanin synthesis in eggplant[M. D. Dissertation]. Shanghai: Shanghai Jiao Tong University. (in Chinese)
	邵文婷. 2013. 茄子花青素合成相关基因SmMYB的克隆与功能研究[硕士论文]. 上海: 上海交通大学.
[26]	Stommel R, Judith M. 2015. Coordinated regulation of biosynthetic and regulatory genes coincides with anthocyanin accumulation in developing eggplant fruit. Journal of the American Society for Horticultural Science, 140(2):129-135. doi: 10.21273/JASHS.140.2.129 URL
[27]	Sun Baojuan, Heng Zhou, Li Zhiliang, Li Zhenxing, Li Tao, Gong Chao. 2018. Indel molecular marker closely linked to epistatic gene D of eggplant fruit color and its application. Acta Horticulturae Sinica, 45(S1):2612. (in Chinese)
	孙保娟, 衡周, 李植良, 黎振兴, 李涛, 宫超. 2018. 茄子果色上位性基因D紧密连锁的InDel分子标记及其应用. 园艺学报, 45(S1):2612.
[28]	Takagi H, Abe A, Yoshida K, Kosugi S, Natsume S, Mitsuoka C, Uemura A, Utsushi H, Tamiru M, Takuno S. 2013. QTL-seq:rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations. The Plant Journal, 74:174-183. doi: 10.1111/tpj.2013.74.issue-1 URL
[29]	Toppino L, Barchi L, Lo Scalzo R, Palazzolo E, Francese G, Fibiani M, D'Alessandro A, Papa V, Laudicina V A, Sabatino L, Pulcini L, Sala T, Acciarri N, Portis E, Lanteri S, Mennella G, Rotino G L. 2016. Mapping quantitative trait loci affecting biochemical and morphological fruit properties in eggplant(Solanum melongena L.). Frontiers in Plant Science, 7:256. doi: 10.3389/fpls.2016.00256 pmid: 26973692
[30]	Xiang Congchao, Zhang Weiwei, Luo Shuangxia, Zhao Jianjun, Zhang Tingting, Shen Shuxing, Chen Xueping. 2015. Genetic analysis of color under calyx of eggplant and correlation analysis of SSR markers. Journal of Hebei Agricultural University, 38(4):50-55. (in Chinese)
	相丛超, 张巍巍, 罗双霞, 赵建军, 张婷婷, 申书兴, 陈雪平. 2015. 茄子果萼下颜色的遗传分析和SSR标记的相关性分析. 河北农业大学学报, 38(4):50-55.
[31]	Xu Huaxia, Li Peijun, Liu Wan, Song Yufang. 2002. Research progress of cytochrome P450. Journal of Agricultural Environmental Sciences, 21(2):188-191. (in Chinese)
	许华夏, 李培军, 刘宛, 宋玉芳. 2002. 生物细胞色素P450的研究进展. 农业环境科学学报, 21(2):188-191.
[32]	Wang Shijie. 2016. Screening and cloning of key MYB transcription factor of anthocyanin biosynthesis in eggplant[M. D. Dissertation]. Tai'an: Shandong Agricultural University. (in Chinese)
	王世界. 2016. 茄子花青素生物合成关键MYB转录因子的筛选及克隆[硕士论文]. 泰安: 山东农业大学.
[33]	Zhang Y, Hu Z, Chu C, Huang C, Tian S, Zhao Z, Chen G. 2014. Anthocyanin accumulation and molecular analysis of anthocyanin biosynthesis-associated genes in eggplant(Solanum melongena L.). Journal of Agricultural & Food Chemistry, 62(13):2906-2912.
[34]	Zhang Ying, Zhao Yueqi, Chen Yuhui, Lian Yong, Liu Fuzhong. 2019. Progress in molecular research on purple formation of eggplant. Acta Horticulturae Sinica, 46(9):1779-1796. (in Chinese)
	张映, 赵悦琪, 陈钰辉, 连勇, 刘富中. 2019. 茄子紫色形成的分子研究进展. 园艺学报, 46(9):1779-1796.
[35]	Zhang Yuanyuan. 2020. Structure and signal regulation mechanism of phytochrome. Hubei Agricultural Science, 59(4):5-10. (in Chinese)
	张媛媛. 2020. 光敏色素的结构及其信号调控机制. 湖北农业科学, 59(4):5-10.

预测基因 Predicted gene	染色体 Chr.	关联区间/Mb Correlation interval	突变位置 Mutation location	突变碱基 Mutant base	突变区域 Mutation region	是否同义突变 Synonymous mutation（Y/N）	基因注释 Gene annotation
EGP21397	10	1.9 ~ 5.1	2 045 972	G→A	外显子区 Extron	否N	C2H2型锌指蛋白 C2HC zinc fingers superfamily protein
EGP21400	10	1.9 ~ 5.1	2 112 411	A→T	外显子区 Extron	否N	钙调素结合家族蛋白 Calmodulin-binding family protein
EGP21491	10	1.9 ~ 5.1	3 244 885	T→C	外显子区 Extron	否N	类胡萝卜素异构酶 Carotenoid isomerase
EGP21416	10	1.9 ~ 5.1	2 279 620	A→G	基因间 Intergenic	否N	细胞色素P450,家族96,亚家族A,多肽1 Cytochrome P450,family 96,subfamily A,polypeptide 1
EGP21417	10	1.9 ~ 5.1	2 279 620	A→G	基因间 Intergenic	否N	细胞色素P450,家族96,亚家族A,多肽1 Cytochrome P450,family 96,subfamily A,polypeptide 1
EGP21480	10	1.9 ~ 5.1	3 107 257	G→A	基因间 Intergenic	否N	细胞色素P450,家族82,亚家族C,多肽4 Cytochrome P450,family 82,subfamily C,polypeptide 4
EGP21561	10	1.9 ~ 5.1	3 901 677	G→A	基因间 Intergenic	否N	细胞色素P450,家族94,亚家族C,多肽1 Cytochrome P450,family 94,subfamily C, polypeptide 1
EGP21596	10	1.9 ~ 5.1	4 258 708	C→A	基因间 Intergenic	否N	细胞色素P450,家族76,亚家族C,多肽7 Cytochrome P450,family 76,subfamily C, polypeptide 7
EGP21891	10	7.3 ~ 14.1	8 710 042	G→A	基因间 Intergenic	否N	光敏色素 Phytochrome
EGP22288	10	14.5 ~ 23.7	17 833 364	A→G	基因间 Intergenic	否N	2Fe-2S铁氧还蛋白样超家族蛋白 2Fe-2S ferredoxin-like superfamily protein
EGP22289	10	14.5 ~ 23.7	17 833 364	A→G	基因间 Intergenic	否N	脂质转运蛋白 Lipid transfer protein

预测基因 Predicted gene	染色体 Chr.	关联区间/Mb Correlation interval	突变位置 Mutation location	突变碱基 Mutant base	突变区域 Mutation region	是否同义突变 Synonymous mutation（Y/N）	基因注释 Gene annotation
EGP21397	10	1.9 ~ 5.1	2 045 972	G→A	外显子区 Extron	否N	C2H2型锌指蛋白 C2HC zinc fingers superfamily protein
EGP21400	10	1.9 ~ 5.1	2 112 411	A→T	外显子区 Extron	否N	钙调素结合家族蛋白 Calmodulin-binding family protein
EGP21491	10	1.9 ~ 5.1	3 244 885	T→C	外显子区 Extron	否N	类胡萝卜素异构酶 Carotenoid isomerase
EGP21416	10	1.9 ~ 5.1	2 279 620	A→G	基因间 Intergenic	否N	细胞色素P450,家族96,亚家族A,多肽1 Cytochrome P450,family 96,subfamily A,polypeptide 1
EGP21417	10	1.9 ~ 5.1	2 279 620	A→G	基因间 Intergenic	否N	细胞色素P450,家族96,亚家族A,多肽1 Cytochrome P450,family 96,subfamily A,polypeptide 1
EGP21480	10	1.9 ~ 5.1	3 107 257	G→A	基因间 Intergenic	否N	细胞色素P450,家族82,亚家族C,多肽4 Cytochrome P450,family 82,subfamily C,polypeptide 4
EGP21561	10	1.9 ~ 5.1	3 901 677	G→A	基因间 Intergenic	否N	细胞色素P450,家族94,亚家族C,多肽1 Cytochrome P450,family 94,subfamily C, polypeptide 1
EGP21596	10	1.9 ~ 5.1	4 258 708	C→A	基因间 Intergenic	否N	细胞色素P450,家族76,亚家族C,多肽7 Cytochrome P450,family 76,subfamily C, polypeptide 7
EGP21891	10	7.3 ~ 14.1	8 710 042	G→A	基因间 Intergenic	否N	光敏色素 Phytochrome
EGP22288	10	14.5 ~ 23.7	17 833 364	A→G	基因间 Intergenic	否N	2Fe-2S铁氧还蛋白样超家族蛋白 2Fe-2S ferredoxin-like superfamily protein
EGP22289	10	14.5 ~ 23.7	17 833 364	A→G	基因间 Intergenic	否N	脂质转运蛋白 Lipid transfer protein

Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 35

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Zhiliang, LI Zhenxing, LI Tao, SUN Baojuan, LI Ying, XU Xiaowan, WANG Hengming, HENG Zhou, GONG Chao. A New Eggplant Cultivar‘Xinfeng 3’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 227-228.
[2]	CAI Peng, FANG Chao, LI Yuejian, LIU Duchen, LIU Xiaojun, LIANG Genyun. A New Eggplant Cultivar‘Tianjiao’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 229-230.
[3]	TAN Jie, HUANG Shuping, CHEN Xia, ZHANG Hongyuan, LI Ye, WANG Benqi, CHEN Hao, WU Xuexia, and ZHANG Min, . A New Eggplant Cultivar‘Eqie 5’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 99-100.
[4]	HUANG Shuping, TAN Jie, Chen Xia, ZHANG Hongyuan, LI Ye, WANG Benqi, CHEN Hao, WU Xuexia, and ZHANG Min, . A New Eggplant Cultivar‘Eqie 6’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 101-102.
[5]	YANG Yang, TIAN Shibing, WANG Yongqing, JIANG Changchun, ZOU Min, TAO Tao, ZHOU Shanshan, WANG Zhijin, BAO Zhongxian, and TANG Xiaohua. A New Eggplant Cultivar‘Yuqie 5’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 77-78.
[6]	LIN Yuanmi, ZHU Wenjiao, CHEN Min, XUE Chunmei, JIN Fangyu, ZHU Yuping, JIANG Xinyue, YE Lingfeng, NI Shunanling, YANG Qing. Mir396b Negatively Regulates Eggplant Defense Response to Verticillium Wilt [J]. Acta Horticulturae Sinica, 2022, 49(8): 1713-1722.
[7]	WANG Dan, WANG Mi, LIU Jun, ZHOU Xiaohui, LIU Songyu, YANG Yan, ZHUANG Yong. Cloning of U6 Promoters and Establishment of CRISPR/Cas9 Mediated Gene Editing System in Eggplant [J]. Acta Horticulturae Sinica, 2022, 49(4): 791-800.
[8]	SHENG Yunyan, YANG Limin, DAI Dongyang, ZHANG Jiaxin, WANG Ling, WANG Di, CAI Yi, TIAN Limei. Cytological Observation of Fruit Peduncle Abscission Zone and Preliminary Mapping of Mature Fruit Abscission AL3 gene in Melon [J]. Acta Horticulturae Sinica, 2022, 49(2): 341-351.
[9]	LIN Hui, XUE Zhuzheng, HUANG Jiandu, CHEN Jibing, WEN Qingfang. A New Eggplant Cultivar‘Fuqie 8’ [J]. Acta Horticulturae Sinica, 2022, 49(2): 463-464.
[10]	QIAO Jun, WANG Liying, LIU Jing, LI Suweng. Expression Analysis of Genes Related to Photosensitive Color Under the Caylx in Eggplant Based on Transcriptome Sequencing [J]. Acta Horticulturae Sinica, 2022, 49(11): 2347-2356.
[11]	LI Zhiliang, LI Zhenxing, LI Tao, SUN Baojuan, LI Ying, XU Xiaowan, WANG Hengming, HENG Zhou, and GONG Chao. A New Eggplant Cultivar'Xinfeng 2' [J]. Acta Horticulturae Sinica, 2022, 49(10): 2289-2290.
[12]	JIA Li, JIANG Haikun, WANG Yan, YAN Congsheng, WANG Mingxia, DONG Yanxiang, FANG Ling, ZHANG Qi’an, YU Feifei, and GE Zhihuan. A New White Eggplant Cultivar‘Wanqie 048’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2837-2838.
[13]	YANG Shuangjuan, Zhang Xiaowei, WEI Xiaochun, ZHAO Yanyan, WANG Zhiyong, ZHAO Xiaobin, LI Lin, YUAN Yuxiang. Mapping and KASP Markers Development for Clubroot Resistance Gene BraA.Pb.8.4 in Chinese Cabbage [J]. Acta Horticulturae Sinica, 2021, 48(7): 1317-1328.
[14]	CHENG Feng, SONG Mengfei, CAO Lei, ZHANG Mengru, YANG Zhige, CHEN Jinfeng, LOU Qunfeng. Genetic Mapping for a Medium Short-fruit Mutant of Cucumber [J]. Acta Horticulturae Sinica, 2021, 48(7): 1359-1370.
[15]	YU Hailong, REN Wenjing, FANG Zhiyuan, YANG Limei, ZHUANG Mu, LÜ Honghao, WANG Yong, JI Jialei, ZHANG Yangyong. Advances on Fertility Restoration for Cytoplasmic Male Sterility in Vegetables [J]. Acta Horticulturae Sinica, 2021, 48(5): 1031-1046.