Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 103-115.doi: 10.16420/j.issn.0513-353x.2021-0919

• Research Papers • Previous Articles     Next Articles

Association Analysis of Organ Traits with SSR Markers in Lotus(Nelumbo nucifera

LIU Yiping1,2, NI Menghui1, WU Fangfang1, LIU Hongli1,2, HE Dan1,2, KONG Dezheng1,2,*()   

  1. 1College of Landscape Architecture and Art,Henan Agricultural University,Zhengzhou 450002,China
    2Henan High-quality Flower and Vegetable Seedling Engineering Technology Center,Zhengzhou 450002,China
  • Received:2022-09-14 Revised:2022-11-01 Online:2023-01-25 Published:2023-01-18
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In order to discover the molecular markers related to the organ traits of lotus flowers,the 18 floral organ traits of 60 lotus germplasm resources were identified and described,16 polymorphic SSR markers were screened to analyze their genetic diversity,and use the generalized linear model(GLM)and mixed linear model(MLM)of TASSEL5.0 software to carry out correlation analysis on 18 traits of floral organs. The results of the study showed that the 16 SSR markers were mainly dinucleotide repeats and trinucleotide repeats,both accounting for 43.75%;105 polymorphic loci were amplified in 60 lotus germplasm materials,with an average number of 6.5625;the primer polymorphism content(PIC)ranged from 0.2898 to 0.7029,with an average value of 0.5242;the average value of Shannon’s information index was 1.1548;and the average value of observed heterozygosity and expected heterozygosity were 0.4308 and 0.5819. The genetic similarity coefficient(GS)of 60 lotus cultivars was from 0.38 to 0.92,with an average value of 0.67. At the GS value of 0.60,60 lotus germplasms were divided into three major populations. GLM model analysis detected 31 SSR loci,with the phenotypic interpretation rate ranged from 15.72% to 76.76%;MLM model analysis detected 13 SSR loci,with a phenotypic interpretation rate ranged from 20.25% to 78.40%;ten of them were detected by both. In addition,nine SSR-labeled loci were detected in 2-5 floral organ traits,showing a significant“one cause and multiple effects”phenomenon.

Key words: lotus, floral organ, trait, SSR molecular marker, association analysis

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