Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 307-318.doi: 10.16420/j.issn.0513-353x.2021-1046

• Research Papers • Previous Articles     Next Articles

Establishment of SNP Fingerprinting and Identification of Chinese Flowering Cabbage Varieties Based on KASP Genotyping

REN Hailong, XU Donglin*(), ZHANG Jing*(), ZOU Jiwen, LI Guangguang, ZHOU Xianyu, XIAO Wanyu, SUN Yijia   

  1. Guangzhou Academy of Agricultural Sciences,Guangzhou 510308,China
  • Received:2022-09-14 Revised:2022-12-10 Online:2023-02-25 Published:2023-03-06
  • Contact: *(


In this study,200 KASP-SNP markers previously developed from Brassica rapa were estimated using 89 Chinese flowering cabbage varieties. The results showed that 131 of tested markers were successful in the genotyping,with conversion rate 65.50%,deletion rate 0.06,minor allele frequency(MAF)0.21,observed heterozygosity 0.27 and polymorphism information content(PIC)0.21. These markers were used for phylogenetic tree construction and genetic structure analysis. Further,they were screened based on the criteria-minor allele frequency > 0.25,polymorphism information content > 0.35,deletion rate < 0.03,and heterozygosity rate < 0.6 and 18 of them were selected as core KASP-SNP markers. Using these 18 markers,a fingerprinting for the 89 Chinese flowering cabbage varieties was constructed,demonstrating the feasibility of KASP technique in identification of Chinese flowering cabbage varieties.

Key words: Chinese flowering cabbage, KASP, fingerprinting, variety identification

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