Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 175-186.doi: 10.16420/j.issn.0513-353x.2021-0312

• Research Notes • Previous Articles     Next Articles

Genetic Diversity Analysis and Core Collection of Cymbidium ensifolium Germplasm Resources

CHEN Mingkun1, CHEN Lu1, SUN Weihong2, MA Shanhu1, LAN Siren1, PENG Donghui1, LIU Zhongjian1, AI Ye1,*()   

  1. 1Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    2College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Received:2021-05-09 Revised:2021-11-26 Online:2022-01-25 Published:2022-01-24
  • Contact: AI Ye


The phenotypic characteristics of sepal color,petal color,lip color,leaf art,petal type and lip spot of 311 Cymbidium ensifolium germplasms were recorded,and the traits were assigned and statistically analyzed. Twenty-two pairs of SSR fluorescent primers were used to perform PCR amplification on 311 C. ensifolium germplasm resources in order to conduct genetic diversity analysis. Use Structure software to analyze the population structure of C. ensifolium germplasm resources,and principal component analysis and cluster analysis were employed for verification. Core collection was analyzed and screened by using Core Finder software and t-test. The results showed that the phenotypic genetic diversity of the six quality traits of 311 C. ensifolium germplasm resources were all greater than 0.5,indicating that the phenotype of C. ensifolium germplasm resources had abundant genetic variations. A total of 171 alleles(Na)were detected by 22 pairs of SSR fluorescent primers,the average of Ne was 2.729. High genetic diversity was revealed in the germplasm resources of C. ensifoliumI = 1.026). Structure software analysis results showed that the optimal population number K was three,and there is a small amount of germplasm mixing among the populations. The population structure analysis divided C. ensifolium germplasm resources into three groups. Principal component analysis and cluster analysis further verified the reliability of its classification. In this study,51 core collections of C. ensifolium were constructed,accounting for 16.4% of the initial collection,with the retention rate of Na,Ne,and I were 100%,130% and 124%,respectively. Through t-test and principal coordinate analysis showed that there was no significant difference between the genetic diversity of the core collection and initial collection. The 51 core collections constructed could represent the genetic diversity of C. ensifolium germplasm resources to the greatest extent.

Key words: Cymbidium ensifolium, germplasm resources, phenotypic traits, SSR, genetic diversity, core collection

CLC Number: