Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 265-278.doi: 10.16420/j.issn.0513-353x.2021-1008

• Research Papers • Previous Articles     Next Articles

Construction of SSR Fingerprints of Pomegranate Cultivars and Male Parent Identification of Hybrids

WANG Rui1,*, HONG Wenjuan1,*, LUO Hua2, ZHAO Lina2, CHEN Ying2, WANG Jun1,**()   

  1. 1. National Engineering Research Center of Tree Breeding and Ecological Restoration,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China
    2. National Germplasm Bank of Pomegranate,Zaozhuang,Shandong 277300,China
  • Received:2022-08-11 Revised:2022-12-02 Online:2023-02-25 Published:2023-03-06
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Young leaves of 168 pomegranate cultivars collected from the National Forest Germplasm Resource Repository for Pomegranate,Zaozhuang City,Shandong,China were used to construct their fingerprints using SSR molecular marker technique. Based on the fingerprint information,the maximum likelihood method(LOD)was applied to hybrid screening in open-pollinated progenies of the cultivars ‘Qiuyan’and‘Huili Qingpi Ruanzi’and identification of suspected male parent. The results showed that a total of 76 alleles were amplified from 22 pairs of SSR primers in the 168 cultivar samples,including 58 polymorphic loci(accounting for 76.3%). The average number of alleles per locus(Na),effective number of alleles(Ne),observed heterozygosity(Ho)and expected heterozygosity(He)was 3.455,2.269,0.362 and 0.538,respectively. The average Shannon’s information index(I)was 0.897,the polymorphism information content(PIC)of each marker ranged from 0.330 to 0.692,with an average of 0.462. 166 pomegranate cultivar samples were distinguished by the 22 pairs of SSR primers. However,‘Damantianhongtian’and‘Xiaomantianhongtian’were not effectively distinguished,which might be synonymous. Using the allelic information,a database containing the specific fingerprint code and two-dimensional fingerprint code for each cultivar was constructed. One true hybrid Q-1 was screened from 30 offspring of open-pollinated‘Qiuyan’by using the fingerprint information. Its suspected male parent is‘Yicheng Fengchan Maya’,with the LOD value of 5.96. Six true hybrids,H-4,H-49,H-51,H-87,H-94 and H-103,were screened from 118 offspring of open-pollinated‘Huili Qingpi Ruanzi’. Their suspected male parents were‘Sichuan Huangpisuan’‘Tunisian Ruanzi’‘Mengzi Honghua Baipi’‘Sichuan Huangpisuan’‘Tunisian Ruanzi’and‘Mengzi Honghua Baipi’,respectively.

Key words: Punica granatum, cultivar resource, SSR fingerprint, genetic diversity, male parent identification

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