Analysis on the Resistance to Zucchini Yellow Mosaic Virus in Watermelon Inbred Line 938-16-B

doi:10.16420/j.issn.0513-353x.2021-0067

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 633-640.doi: 10.16420/j.issn.0513-353x.2021-0067

• Research Notes • Previous Articles Next Articles

Analysis on the Resistance to Zucchini Yellow Mosaic Virus in Watermelon Inbred Line 938-16-B

BAI Erjun, LIU Liming, Hao Xiaoyuan, PENG Bin, WU Huijie, GU Qinsheng^*(), KANG Baoshan^*()

Henan Key Laboratory Fruit and Cucurbit Biology,Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450000,China

Received:2021-03-30 Revised:2021-11-12 Online:2022-03-25 Published:2022-03-25
Contact: GU Qinsheng,KANG Baoshan E-mail:guqinsheng@caas.cn;kangbaoshan@caas.cn

Abstract

Abstract:

Zucchini yellow mosaic virus is a serious emerging virus that has threatened the economically important cucurbits industry in major production areas worldwide. Watermelon inbred line 938-16-B had been identified as resistance to ZYMV in our previous work. It is characterized by hypersensitive response（HR）after infection by ZYMV. Here,we investigated the inheritance of resistance to ZYMV in 938-16-B. In this study,F₂ generation with 310 individuals and BC₁ generation were generated through crossing 938-16-B with ZYMV-susceptible inbred line H1. According to the segregation ratio of resistance in BC₁ and F₂ generation,ZYMV resistance in 938-16-B would be established. During the process of inoculation,we found hypersensitive response（HR）would not be induced when the temperature was over 30 ℃,which suggested the resistant gene was temperature-sensitive. Therefore,identification of resistance in the F₂ and BC₁ population was carried out under the condition of 23-28 ℃. Results showed that,the segregation ratios of resistance to susceptibility in F₂ and BC₁ were 3︰1 and 1︰1,respectively,which revealed the resistance to ZYMV in watermelon is controlled by a single dominant gene.

Key words: watermelon, zucchini yellow mosaic virus, hypersensitive response, genetic law

CLC Number:

S651

BAI Erjun, LIU Liming, Hao Xiaoyuan, PENG Bin, WU Huijie, GU Qinsheng, KANG Baoshan. Analysis on the Resistance to Zucchini Yellow Mosaic Virus in Watermelon Inbred Line 938-16-B[J]. Acta Horticulturae Sinica, 2022, 49(3): 633-640.

Figures/Tables 5

References 31

[1]	Amano M, Mochizuki A, Kawagoe Y, Iwahori K, Niwa K, Svoboda J, Maeda T, Imura Y. 2013. High-resolution mapping of zym,a recessive gene for zucchini yellow mosaic virus resistance in cucumber. Theoretical and Applied Genetics, 126(12):2983-2993. doi: 10.1007/s00122-013-2187-5 URL
[2]	Blua M J, Perring T M. 1989. Effect of zucchini yellow mosaic-virus on developmentand yield of cantaloupe(Cucumis melo). Plant Disease, 3(73):111-115.
[3]	Brandes J, Wetter C. 1959. Classification of elongated plant viruses on the basis of particle morphology. Virology, 8(1):99-115. doi: 10.1016/0042-6822(59)90022-4 URL
[4]	Capuozzo C, Formisano G, Iovieno P, Andolfo G, Tomassoli L, Barbella M M, Pico B, Paris H S, Ercolano M R. 2017. Inheritance analysis and identification of SNP markers associated with ZYMV resistance in Cucurbita pepo. Molecular Breeding, 37(8):99. doi: 10.1007/s11032-017-0698-5 URL
[5]	Chen H, Arsovski A A, Yu K, Wang A. 2016. Deep sequencing leads to the identification of eukaryotic translation initiation factor 5A as a key element in Rsv1-mediated lethal systemic hypersensitive response to Soybean mosaic virus infection in soybean. Molecular Plant Pathology, 18(3):391-404 doi: 10.1111/mpp.12407 URL
[6]	Coutts B A, Kehoe M A, Jones R A. 2011. Minimising losses caused by Zucchini yellow mosaic virus in vegetable cucurbit crops in tropical,sub-tropical and Mediterranean environments through cultural methods and host resistance. Virus Research, 159(2):141-160. doi: 10.1016/j.virusres.2011.04.015 pmid: 21549770
[7]	Danin-Poleg Y, Paris H S, Cohen S, Rabinowitch H D, Karchi Z. 1997. Oligogenic inheritance of resistance to zucchini yellow mosaic virus in melons. Euphytica, 93(3):331-337. doi: 10.1023/A:1002944432083 URL
[8]	Desbiez C, Gal-On A, Girard M, Wipf-Scheibel C, Lecoq H. 2003. Increase in Zucchini yellow mosaic virus symptom severity in tolerant zucchini cultivars is related to a point mutation in P3 protein and is associated with a loss of relative fitness on susceptible plants. Phytopathology, 93(12):1478-1484. doi: 10.1094/PHYTO.2003.93.12.1478 pmid: 18943611
[9]	Desbiez C, Lecoq H. 1997. Zucchini yellow mosaic virus. Plant Pathology, 46:809-829. doi: 10.1046/j.1365-3059.1997.d01-87.x URL
[10]	Dinesh-Kumar S P, Whitham S, Choi D, Hehl R, Corr C. 1995. Transposon tagging of tobacco mosaic virus resistance gene N:its possible role in the TMV-N-mediated signal transduction pathway. Proc Natl Acad Sci USA, 92(10):4175-4180. doi: 10.1073/pnas.92.10.4175 URL
[11]	Guner N, Pesic-VanEsbroeck Z, Rivera-Burgos L A, Wehner T C. 2019. Screening for resistance to Zucchini yellow mosaic virus in the watermelon germplasm. HortScience, 54(2):206-211. doi: 10.21273/HORTSCI13325-18 URL
[12]	Jia Hai-yan, Song Li-yun, Xu Xiang, Xie Yi, Zhang Chao-qun, Liu Tian-bo, Zhao Cun-xiao, Shen Li-li, Wang Jie, Li Ying, Wang Feng-long, Yang Jin-guang. 2020. Differential expression of LncRNAs in Nicotiana tabacum var. samsun NN infected by TMV at different temperatures. Scientia Agriculture Sinica, 53(7):1381-1396. (in Chinese)
	贾海燕, 宋丽云, 徐翔, 解屹, 张超群, 刘天波, 赵存孝, 申莉莉, 王杰, 李莹, 王凤龙, 杨金广. 2020. 不同温度下TMV侵染枯斑三生烟的LncRNA差异表达. 中国农业科学, 53(7):1381-1396.
[13]	Lecoq H, Desbiez C. 2008. Watermelon mosaic virus and zucchini yellow mosaic virus//Encyclopedia of Virology. 3rd ed. Oxford: Academic Press:433-440.
[14]	Lecoq H, Katis N. 2014. Control of cucurbit viruses. Advances in Virus Research, 90:255-296.
[15]	Li D X, Chen P Y, Shi A N, Shakiba E, Gergerich R, Chen Y F. 2009. Temperature affects expression of symptoms induced by soybean mosaic virus in homozygous and heterozygous plants. Journal of Heredity, 100(3):348-354. doi: 10.1093/jhered/esn109 URL
[16]	Li F, Pignatta D, Bendix C, Brunkard J O, Cohn M M, Tung J, Sun H, Kumar P, Baker B. 2012. Microrna regulation of plant innate immune receptors. Proceedings of the National Academy of Sciences, 109:1790-1795.
[17]	Li Hai-zhen, Zhang Guo-yu, Zhang Fan, Jia Chang-cai, Jiang Li-gang. 2014. A new summer squash F₁ hybrid with high resistance to ZYMV—‘Jinghu CRV4’. China Vegetables, (1):49-51. (in Chinese)
	李海真, 张国裕, 张帆, 贾长才, 姜立纲. 2014. 抗ZYMV西葫芦新品种京葫CRV4的选育. 中国蔬菜, (1):49-51.
[18]	Li Jia-wei, Lin Ming, Hu Hong, Wang Xiao-xuan, Guo Yan-mei, Huang Ze-jun, Du Yong-chen, Gao Jian-chang. 2016. The combined effects of different TYLCV resistant genes and temperatures on TYLCV duplication. Acta Horticulturae Sinica, 43(1):71-79. (in Chinese)
	李佳蔚, 林铭, 胡鸿, 王孝宣, 国艳梅, 黄泽军, 杜永臣, 高建昌. 2016. 番茄抗黄化曲叶病基因在不同温度下对TYLCV复制的影响. 园艺学报, 43(1):71-79.
[19]	Lisa V, Boccardo G, D'Agostino G, Dellavalle G, D'Aquilio M. 1981. Characterization of potyvirus that causes zucchini yellow mosaic. Phytopathology, 71(7):667-666. doi: 10.1094/Phyto-71-667 URL
[20]	Novakova S, Flores-Ramirez G, Glasa M, Danchenko M, Fiala R, Skultety L. 2015. Partially resistant Cucurbita pepo showed late onset of the Zucchini yellow mosaic virus infection due to rapid activation of defense mechanisms as compared to susceptible cultivar. Frontiers in Plant Science, 6:263.
[21]	Paris H S, Cohen S, Burger Y, Yoseph R. 1988. Single-gene resistance to Zucchini yellow mosaic virus in Cucurbita moschata. Euphytica, 37(1):27-29. doi: 10.1007/BF00037219 URL
[22]	Pitrat M, Lecoq H. 1984. Inheritance of zucchini yellow mosaic virus resistance in Cucumis melol. Euphytica, 33(1):57-61. doi: 10.1007/BF00022750 URL
[23]	Provvidenti R. 1987. Inheritance of resistance to a strain of zucchini yellow mosaic virus in cucumber. HortScience, 22:102-103.
[24]	Szajko K, Strzelczyk-Zyta D, Marczewski W. 2014. Ny-1 and Ny-2 genes conferring hypersensitive response to potato virus Y(PVY)in cultivated potatoes:mapping and marker-assisted selection validation for PVY resistance in potato breeding. Molecular Breeding, 34(1):267-271. doi: 10.1007/s11032-014-0024-4 URL
[25]	Szajko K, Yin Z, Marczewski W. 2019. Accumulation of miRNA and mRNA targets in potato leaves displaying temperature-dependent responses to potato virus Y. Potato Research, 62(4):379-392. doi: 10.1007/s11540-019-9417-4 URL
[26]	Szajko K. Chrzanowska M, Witek K, Strzelczyk-Zyta D, Zagorska H, Gebhardt C, Hennig J, Marczewski W. 2008. The novel gene Ny-1 on potato chromosome IX confers hypersensitive resistance to Potato virus Y and is an alternative to Ry genes in potato breeding for PVY resistance. Theor Appl Genet, 116(2):297-303. doi: 10.1007/s00122-007-0667-1 pmid: 17985110
[27]	Wang Jian, Ji Shuxian, Wang Ying, Geng Chao, Li Xiangdong, Tian Yanping. 2019. Complete genomic sequence analysis of Zucchini yellow mosaic virus pumpkin and loofah isolates from Shandong Province. Acta Horticulturae Sinica, 46(4):784-796. (in Chinese)
	王健, 冀树娴, 王莹, 耿超, 李向东, 田延平. 2019. 小西葫芦黄花叶病毒山东南瓜和丝瓜分离物全基因组序列分析. 园艺学报, 46(4):784-796.
[28]	Wang Yin-lei, Zhao Tong-min, Yang Wen-cai, Shen Huo-lin. 2014. Efect of temperatures on expression of tomato root-knot nematode resistance related genes. Jiangsu J Agr Sci, 30(6):1417-1422. (in Chinese)
	王银磊, 赵统敏, 杨文才, 沈火林. 2014. 温度对番茄根结线虫抗性相关基因表达的影响. 江苏农业学报, 30(6):1417-1422.
[29]	Xu Y, Kang D, Shi Z, Shen H, Wehner T. 2004. Inheritance of resistance to zucchini yellow mosaic virus and watermelon mosaic virus in watermelon. Journal of Heredity, 95(6):498-502. pmid: 15475395
[30]	Zheng Guang-yu, Dong Tao. 1991. Occurrence of zucchini yellow mosaic virus in xinjiang. Acta Phytopathologica Sinica, (1):74. (in Chinese)
	郑光宇, 董涛. 1991. 在新疆发生的小西葫芦黄化花叶病毒的研究初报. 植物病理学报, (1):74.
[31]	Zhu Y, Qian W Q, Hua J. 2010. Temperature modulates plant defense responses through NB-LRR proteins. PLoS Pathogens, 6(4):e1000844.

群体 Population	光照强度/% Light intensity	总株数 Total	抗病株 Resistant plant
F₂-9	60 ~ 100	9	7
	20 ~ 60	8	7
	13 ~ 20	10	影响生长 Growth being limited
F₂-10	60 ~ 100	7	6
	20 ~ 60	8	7
	13 ~ 20	10	影响生长 Growth being limited

群体 Population	光照强度/% Light intensity	总株数 Total	抗病株 Resistant plant
F₂-9	60 ~ 100	9	7
	20 ~ 60	8	7
	13 ~ 20	10	影响生长 Growth being limited
F₂-10	60 ~ 100	7	6
	20 ~ 60	8	7
	13 ~ 20	10	影响生长 Growth being limited

群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plant	群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plant
F₂-1	30 ~ 40	9	2	F₂-6	30 ~ 40	9	4
	23 ~ 28	5	5		23 ~ 28	8	8
F₂-2	30 ~ 40	9	2	F₂-7	30 ~ 40	9	2
	23 ~ 28	8	5		23 ~ 28	9	7
F₂-3	30 ~ 40	9	4	F₂-8	30 ~ 40	8	1
	23 ~ 28	6	6		23 ~ 28	7	4
F₂-4	30 ~ 40	8	3	F₂-9	30 ~ 40	10	0
	23 ~ 28	8	7		23 ~ 28	9	7
F₂-5	30 ~ 40	9	1	F₂-10	30 ~ 40	10	0
	23 ~ 28	8	7		23 ~ 28	7	6

群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plant	群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plant
F₂-1	30 ~ 40	9	2	F₂-6	30 ~ 40	9	4
	23 ~ 28	5	5		23 ~ 28	8	8
F₂-2	30 ~ 40	9	2	F₂-7	30 ~ 40	9	2
	23 ~ 28	8	5		23 ~ 28	9	7
F₂-3	30 ~ 40	9	4	F₂-8	30 ~ 40	8	1
	23 ~ 28	6	6		23 ~ 28	7	4
F₂-4	30 ~ 40	8	3	F₂-9	30 ~ 40	10	0
	23 ~ 28	8	7		23 ~ 28	9	7
F₂-5	30 ~ 40	9	1	F₂-10	30 ~ 40	10	0
	23 ~ 28	8	7		23 ~ 28	7	6

群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plants	群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plants
P₁(938-16-B)	30 ~ 35	9	7	F₂-9	30 ~ 35	14	1
	23 ~ 28	10	10		23 ~ 28	9	7
	18 ~ 23	11	11		18 ~ 23	10	9
F₁	30 ~ 35	9	2	F₂-10	30 ~ 35	19	0
	23 ~ 28	5	5		23 ~ 28	7	6
	18 ~ 23	2	2		18 ~ 23	6	6

Analysis on the Resistance to Zucchini Yellow Mosaic Virus in Watermelon Inbred Line 938-16-B

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

亲本和后代群体 Parent or population	抗病株数 Number of resistant plants	感病株数 Number of susceptible plants	实际比值 Actual ratio	理论比值 Expected ratio	χ²	P
P₁（938-16-B）	49	0	1︰0	1︰0
P₂（H1）	0	37	0︰1	0︰1
F₁	14	0	1︰0	1︰0
F₂	227	83	2.73︰1	3︰1	0.520	0.470
BC₁	13	12	1︰1	1︰1	0.040	0.841

[1]	WANG Xiqing, JIA Yunhe, YAN Wen, FU Yongkai, YOU Haibo, LI Dongyan, and ZHAO Jingchao. A New Watermelon Cultivar‘Longsheng Jiali’with High Resistance to Fusarium Wilt [J]. Acta Horticulturae Sinica, 2023, 50(2): 455-456.
[2]	XIE Huayun, HE Yi, HONG Rixin, YE Yunfeng, QIN Sihua, HUANG Jinyan, LIU Tangjing, LI Tianyan, LI Guifen, LI Zhi. A New Watermelon Cultivar‘Gui Jinfeng’with Golden Rind [J]. Acta Horticulturae Sinica, 2022, 49(9): 2055-2056.
[3]	XU Binghua, ZHANG Chaoyang, GU Yan, SUN Yudong. A New Watermelon Cultivar‘Suchuang 5’ [J]. Acta Horticulturae Sinica, 2022, 49(2): 465-466.
[4]	XING Nailin, WANG Yinger, YAN Leiyan, GU Binquan, HUANG Yunping, and WANG Yuhong. A New Pumpkin Cultivar‘Strong 7’as a Watermelon Rootstock [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2851-2852.
[5]	ZHANG Haiying, GONG Guoyi, GUO Shaogui, REN Yi, ZHANG Jie, LI Maoying, XU Yong. A New Watermelon Cultivar‘Jingmei 10K’ [J]. Acta Horticulturae Sinica, 2021, 48(7): 1435-1436.
[6]	MI Baobin, XIE Lingling, XIAO Wei, DAI Xiongze, ZHANG Zhuqing, WANG Duanhua, ZHOU Huoqiang. Genetic Law of Fruit and Seed Traits of Wax Gourd and Path Analysis of Single Fruit Weight [J]. Acta Horticulturae Sinica, 2021, 48(12): 2414-2426.
[7]	YUAN Shengkai, MA Cong, YAN Yuemin, LI Guangliang, MA Qiang, ZHU Shuncheng, JIA Zhen, WU Jiang, WANG Xuefen, LI Hongchen, MA Shiyuan, ZHAO Mingqin, MA Changsheng. A New Watermelon Cultivar‘Huazhixiu Bingtang’with Multicolour Flesh [J]. Acta Horticulturae Sinica, 2021, 48(11): 2321-2322.
[8]	HU Junjie, JI Chunhai , HU Yansheng, and LIU Chang. A New Watermelon Cultivar ‘Jike 20’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 3005-3006.
[9]	ZHANG Jie, GONG Guoyi, ZHANG Haiying, GUO Shaogui, REN Yi, LI Maoying, YU Yongtao, and XU Yong. A New Watermelon Cultivar‘Huaxin’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 3007-3008.
[10]	REN Yi, GONG Guoyi, ZHANG Haiying, GUO Shaogui, ZHANG Jie, LI Maoying, YU Yongtao, and XU Yong. A New Mini Watermelon Cultivar ‘Jingmei 2k’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 3009-3010.
[11]	YUAN Shengkai, LI Xutao, ZHANG Baojin, ZHANG Xiaoping, LI Jianhua, LI Hongliang, ZHAO Yongwei, YAO Jian, ZHAO Mingqin, and MA Changsheng, . A New Small-medium Sized Watermelon Cultivar ‘Guazhibao 6’ with Thin Skin [J]. Acta Horticulturae Sinica, 2020, 47(S2): 3011-3012.
[12]	MA Jianxiang，ZHANG Xian*，ZHANG Yong，LI Hao，WEI Chunhua，YANG Jianqiang，XU Nana，and WANG Enyu. A New Watermelon Cultivar‘Shannong 12’ [J]. ACTA HORTICULTURAE SINICA, 2020, 47(7): 1423-1424.
[13]	YAN Congsheng1,3，JIN Jianfang1,3，WANG Mingxia1,3，MA Shaojun1,3，JIA Li1,3，WANG Yan1,3，JIANG Haikun1,3，YU Feifei1,3，FANG Ling1,3，DONG Yanxiang1,3，and ZHANG Qi’an2,3,*. A New Yellow Flesh Watermelon Cultivar‘Yilan’ [J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 807-808.
[14]	YAN Wen, WANG Xiqing, JIA Yunhe, FU Yongkai, and YOU Haibo. Inheritance and Phenotypic Analysis of Small Seed Mutant in Watermelon [J]. Acta Horticulturae Sinica, 2020, 47(11): 2161-2168.
[15]	WANG Xiqing, JIA Yunhe, YAN Wen, FU Yongkai, and YOU Haibo. A New Watermelon Cultivar‘Longsheng 9’with High Resistance to Fusarium Wilt [J]. Acta Horticulturae Sinica, 2020, 47(11): 2273-2274.