Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2313-2324.doi: 10.16420/j.issn.0513-353x.2021-0863

• Research Papars • Previous Articles     Next Articles

Construction of DNA Fingerprint Map and Analysis of Genetic Diversity for Chinese Chestnut Cultivars(Lines)

NIE Xinghua1,2, LI Yiran2, TIAN Shoule3, WANG Xuefeng4, SU Shuchai1, CAO Qingqin2, XING Yu2,*(), QIN Ling2,*()   

  1. 1College of Forestry,Beijing Forestry University,Beijing 100089,China
    2Beijing Key Laboratory for Agricultural Application and New Technique,College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China
    3Shandong Institute of Pomology,Shandong Academy of Agricultural Sciences,Tai’an,Shandong 271000,China
    4Longtan Forest Farm,Liyang,Jiangsu 213300,China
  • Received:2022-04-26 Revised:2022-07-28 Online:2022-11-25 Published:2022-11-25
  • Contact: XING Yu,QIN Ling;


The DNA fingerprint map of 342 chestnut cultivars(lines)was constructed using SSR fluorescent molecular markers as well as the genetic relationship and genetic differentiation were further illuminated among the cultivar groups in the current investigation. The results showed that:(1)The PI and and PIsibs values of all the markers combined were 4.960 × 10-20 and 2.395 × 10-8,respectively,indicating that the 21 markers used in this study had excellent potential for fingerprint recognition. A total of 338 unique corresponding fingerprint information were obtained from 342 cultivars(lines). Among them,‘Shucheng Dahongpao’shared only one fingerprint information with‘Jiaoza’,similarly for ‘Shuanghe Dahongpao’and‘CSB-1’. Combined with the main botanical traits,these cultivars(lines) were finally speculated to be synonym. Simultaneously,19 same-named cultivars(lines)with more than ten loci differences were identified as homonym. (2)The average Fst of each pairwise of chestnut cultivar groups was 0.0224(Fst < 0.05),which demonstrated that there was low genetic differentiation among cultivar groups. Subsequently,gene flow(Nm)and AMOVA were carried out on the five cultivar groups. The genetic variation of chestnut cultivars(lines)mainly was found in individuals,accounting for 84% of the total variation. The average gene flow among cultivar groups was 6.592,revealing extensive gene exchange and restricting differentiation among chestnut cultivar groups.(3)From analyzing the population structure,principal coordinates and cluster tree via the site data,it could be seen that all chestnut cultivars (lines)were mainly divided into two major groups,the north and the south. Meanwhile,a region of northeastern Hubei,southern Henan,and north-central Anhui along the Dabie Mountains and the southern Shandong,the northern Jiangsu constituted the boundary zone(mixed zone)of two groups.

Key words: Chinese chestnut, SSR, fingerprint map, genetic diversity

CLC Number: