Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 187-199.doi: 10.16420/j.issn.0513-353x.2020-0781

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Analysis on Chloroplast Genomic Characteristics of Forsythia suspensa

SONG Yun, JIA Mengjun, CAO Yaping, LI Zheng, HE Jiaxin, WANG Yongfei, ZHANG Xinrui, QIAO Yonggang()   

  1. College of Life Sciences,ShanXi Agricultural University,Taigu,Shanxi 030801,China
  • Received:2021-04-28 Revised:2021-09-09 Online:2022-01-25 Published:2022-01-24
  • Contact: QIAO Yonggang


In order to explore the chloroplasts genomic characteristics of Forsythia suspensa and the phylogenetic development of this genus,high-throughput sequencing technology was used to sequence and functionally annotate the chloroplast genomes of long and short style type in F. suspensa. PCR was used to confirm the sequencing results. The results showed that the chloroplast genomes of the long and short styles type were 156 386 bp,which was a typical four-segment structure in F. suspensa. A total of 114 unique genes were annotated,including 80 protein-coding genes,30 tRNA,and four rRNA. Analysis of 56 simple sequence repeats(SSRs)showed that it mainly composes of A and T bases. In addition,the coding region of the chloroplast genome of F. suspensa prefers to use A and T bases,but the overall codon preference is weak,which is mainly reflected by the difference of Arg and Ser. Based on the chloroplast genome sequence of the heterostyly and 32 plants’ chloroplast genome sequences(including 14 species of Forsythia)published the phylogenetic relationship is analyzed. And the results showed that the chloroplast genome of heterostyly was highly homologous to F. suspensa in Zezhou,Shanxi. It is possible that heterostyly also evolved from the homostyly flowers in F. suspensa.

Key words: Forsythia suspensa, heterostyly, chloroplast genome, SSR, codon preference, phylogenetic analysis

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