Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (9): 1755-1767.doi: 10.16420/j.issn.0513-353x.2020-0953

• Research Papers • Previous Articles     Next Articles

ISSR and rDNA-ITS Sequence Analysis of the Genetic Relationship of Clematis in Hebei Province

WANG Xin, LI Mingyang, TIAN Lin, LIU Dongyun*()   

  1. College of Landscape and Tourism,Hebei Agricultural University,Baoding,Hebei 071000,China
  • Received:2021-01-18 Revised:2021-05-19 Online:2021-09-25 Published:2021-09-30
  • Contact: LIU Dongyun


The ISSR and rDNA-ITS sequence analysis were used to identify the genetic relationship of 17 wild species of Clematis L. in Hebei Province. The ISSR amplified result was conducted cluster analysis by UPGMA method. After PCR amplification,the ITS sequence characteristics were compared and analyzed by Clustal X,MEGA and other software. Finally,the results of these two molecular markers method were comprehensively analyzed. The results showed that primer combinations can amplify 104 polymorphism bands with the percentage of polymorphic bands(PPB)of 100.0%. The average observed number of alleles(Na)was 2.00;the number of effective alleles(Ne)was 1.3069;the Nei’s gene diversity(H)was 0.2196;the Shannon’s information index(I)was 0.3675. According to the clustering results,17 species were divided into five groups at a genetic distance of 0.66. Moreover,the length of ITS sequences varied from 543 to 561 bp,including 87 variable sites,37 information sites and 50 singleton specific discrimination sites in the 17 Clematis L. species. The genetic distance between C. macropetala and C. ochotensis,C. kirilowii and C. kirilowii var. chaneti was 0,and the genetic distance between C. acerfolia and others was large and the genetic relationship was far.

Key words: Clematis, ISSR, ITS, germplasm resource, genetic relationship

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