Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (7): 1295-1303.doi: 10.16420/j.issn.0513-353x.2021-0142

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Accurate Identification of Radish Chromosome F in the Backcross Progeny of Brassica rapa and Rape-radish Chromosome F Addition Line

DING Yunhua1, Budahn Holger2, ZHAO Hong1, ZHAO Xiuyun1,*()   

  1. 1Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Beijing Key Laboratory of Vegetable Germplasm Improvement,Beijing 100097,China
    2Julius Kühn-Institute,Institute for Breeding Research on Horticultural Crops,Quedlinburg D-06484,Germany
  • Received:2021-02-03 Revised:2021-05-21 Online:2021-07-25 Published:2021-08-10
  • Contact: ZHAO Xiuyun


Referring to the SSR molecular markers of chromosome F linkage group in the chromosome-assigned sequence-tagged linkage map of radish,two SSR molecular markers RM-59-293 and Rs1SSR3694-220 which were specific for radish chromosome F were confirmed by means of the known radish-chromosome information in the genetic background of 21 materials. Both SSR markers were used to identify the alien radish chromosome F in the genetic background of BC1 being derived from the backcross of Brassica rapa‘559’and rape-radish chromosome F addition line FF. As a result,10 BC1 plants added with alien radish chromosome F were obtained. Furthermore,the genome of the selected 10 BC1 plants with radish chromosome F were analyzed to detect the exogenous radish chromosome by fluorescence in situ hybridization technology using the radish genomic probe pURsN. The result showed that all of the 10 BC1 had one hybridization signal,which indicated that each of the tested BC1 plants was added with an exogenous radish chromosome. The main agronomic traits of the 10 BC1 plants were further identified. As a result,BC1-15 with the morphology similar to Chinese cabbage parent‘559’and BC1-8,BC1-16,BC1-31 and BC1-35 with the morphology partial to Chinese cabbage were selected.

Key words: Brassica rapa, additional line, radish chromosome F, SSR molecular marker, fluorescent in situ hybridization

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