Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 427-436.doi: 10.16420/j.issn.0513-353x.2020-0929
• Research Notes • Previous Articles Next Articles
SHI Zhaorong1, ZHANG Guangrong2, SUN Shujun2, JIN Mengjun1, CUI Lingxiao1, LIU Yanchao1, WEI Lijuan1, MEI Dahai1, YANG Chengde1,*()
Received:
2021-04-21
Revised:
2021-08-27
Online:
2022-02-25
Published:
2022-02-28
Contact:
YANG Chengde
E-mail:yangcd@gsau.edu.cn
CLC Number:
SHI Zhaorong, ZHANG Guangrong, SUN Shujun, JIN Mengjun, CUI Lingxiao, LIU Yanchao, WEI Lijuan, MEI Dahai, YANG Chengde. Isolation and Identification of Melon Black Spot in Gansu Province[J]. Acta Horticulturae Sinica, 2022, 49(2): 427-436.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0929
基因 Gene | 引物序列(5′-3′) Primer sequence | |
---|---|---|
ITS | ITS1:TCCGTAGGTGAACAYCCTGCG G | ITS4:TCCTCCGCTTATTGATATGC |
CAL | CALDR1:CTTCTGCATCATCAYCTGGACG | CALDF1:AGCAAGTCTCCGAGTTCAAGG |
gpd | gpd-1:CAACGGCTTCGGTCGCATTG | gpd-2:GCCAAGCAGTTGGTTGTGC |
Table 1 Primer information
基因 Gene | 引物序列(5′-3′) Primer sequence | |
---|---|---|
ITS | ITS1:TCCGTAGGTGAACAYCCTGCG G | ITS4:TCCTCCGCTTATTGATATGC |
CAL | CALDR1:CTTCTGCATCATCAYCTGGACG | CALDF1:AGCAAGTCTCCGAGTTCAAGG |
gpd | gpd-1:CAACGGCTTCGGTCGCATTG | gpd-2:GCCAAGCAGTTGGTTGTGC |
Fig. 4 Phylogenetic tree of pathogens based on ITS,CAL and gpd sequences The GenBank accession numbers for nucleotide sequence data are shown in the brackets;Scale is the genetic distance of evolutionary branches.
碳源 Carbon source | 菌落直径/cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/ % Spore germination | 碳源 Carbon source | 菌落直径/ cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/ % Spore germination |
---|---|---|---|---|---|---|---|
木糖 Xylose | 2.47 ± 0.02 e | 13.17 ± 2.46 e | 52.00 ± 3.06 de | 葡萄糖 Glucose | 2.20 ± 0.05 f | 21.67 ± 0.44 d | 90.33 ± 1.45 a |
甘露醇 Mannite | 2.53 ± 0.04 e | 23.50 ± 0.76 d | 69.33 ± 2.03 c | 乳糖 Lactrose | 4.13 ± 0.22 c | 61.50 ± 0.50 a | 82.33 ± 2.03 b |
肌醇 Inosite | 2.83 ± 0.07 d | 24.67 ± 1.01 d | 53.00 ± 4.93 de | 淀粉 Starch | 5.47 ± 0.03 a | 56.50 ± 1.52 a | 49.00 ± 1.52 c |
麦芽糖 Maltose | 4.43 ± 0.01 b | 50.00 ± 0.76 b | 58.67 ± 1.33 d | 蔗糖 Sucrose | 3.03 ± 0.01 d | 43.33 ± 3.94 c | 47.00 ± 0.58 e |
Table 2 The effect of carbon source on mycelium growth,spore production and germination
碳源 Carbon source | 菌落直径/cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/ % Spore germination | 碳源 Carbon source | 菌落直径/ cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/ % Spore germination |
---|---|---|---|---|---|---|---|
木糖 Xylose | 2.47 ± 0.02 e | 13.17 ± 2.46 e | 52.00 ± 3.06 de | 葡萄糖 Glucose | 2.20 ± 0.05 f | 21.67 ± 0.44 d | 90.33 ± 1.45 a |
甘露醇 Mannite | 2.53 ± 0.04 e | 23.50 ± 0.76 d | 69.33 ± 2.03 c | 乳糖 Lactrose | 4.13 ± 0.22 c | 61.50 ± 0.50 a | 82.33 ± 2.03 b |
肌醇 Inosite | 2.83 ± 0.07 d | 24.67 ± 1.01 d | 53.00 ± 4.93 de | 淀粉 Starch | 5.47 ± 0.03 a | 56.50 ± 1.52 a | 49.00 ± 1.52 c |
麦芽糖 Maltose | 4.43 ± 0.01 b | 50.00 ± 0.76 b | 58.67 ± 1.33 d | 蔗糖 Sucrose | 3.03 ± 0.01 d | 43.33 ± 3.94 c | 47.00 ± 0.58 e |
氮源 Nitrogen source | 菌落直径/ cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/ % Spore germination |
---|---|---|---|
氯化铵 Ammonium Chloride | 1.28 ± 0.06 f | 6.67 ± 0.33 e | 32.67 ± 1.76 d |
蛋白胨 Peptone | 5.77 ± 0.05 b | 47.50 ± 2.36 a | 92.33 ± 0.89 a |
尿素 Carbamide | 3.23 ± 0.13 d | 37.83 ± 2.05 bc | 56.33 ± 4.67 b |
硫酸铵 Ammonium Sulfate | 1.83 ± 0.12 e | 0.13 ± 0.02 f | 32.67 ± 1.76 d |
硝酸钾 Potassium Nitrate | 3.68 ± 0.11 c | 38.17 ± 1.59 bc | 52.67 ± 5.46 bc |
酵母膏 Yeast extrac | 6.08 ± 0.02 a | 26.00 ± 0.76 d | 90.33 ± 2.33 a |
牛肉膏 Beef extract | 6.17 ± 0.17 a | 33.50 ± 0.00 c | 51.33 ± 2.19 bc |
硝酸钠 Sodium Nitrate | 3.03 ± 0.01 d | 43.33 ± 3.94 ab | 47.00 ± 0.58 c |
Table 3 The effect of nitrogen source on mycelium growth,spore production and germination
氮源 Nitrogen source | 菌落直径/ cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/ % Spore germination |
---|---|---|---|
氯化铵 Ammonium Chloride | 1.28 ± 0.06 f | 6.67 ± 0.33 e | 32.67 ± 1.76 d |
蛋白胨 Peptone | 5.77 ± 0.05 b | 47.50 ± 2.36 a | 92.33 ± 0.89 a |
尿素 Carbamide | 3.23 ± 0.13 d | 37.83 ± 2.05 bc | 56.33 ± 4.67 b |
硫酸铵 Ammonium Sulfate | 1.83 ± 0.12 e | 0.13 ± 0.02 f | 32.67 ± 1.76 d |
硝酸钾 Potassium Nitrate | 3.68 ± 0.11 c | 38.17 ± 1.59 bc | 52.67 ± 5.46 bc |
酵母膏 Yeast extrac | 6.08 ± 0.02 a | 26.00 ± 0.76 d | 90.33 ± 2.33 a |
牛肉膏 Beef extract | 6.17 ± 0.17 a | 33.50 ± 0.00 c | 51.33 ± 2.19 bc |
硝酸钠 Sodium Nitrate | 3.03 ± 0.01 d | 43.33 ± 3.94 ab | 47.00 ± 0.58 c |
pH | 菌落直径/cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/% Spore germination |
---|---|---|---|
4 | 3.80 ± 0.03 e | 33.33 ± 0.73 d | 69.67 ± 1.33 cd |
5 | 5.58 ± 0.03 d | 38.50 ± 0.87 cd | 79.33 ± 2.40 b |
6 | 5.67 ± 0.04 d | 64.83 ± 1.20 a | 79.67 ± 2.96 b |
7 | 6.87 ± 0.04 a | 54.33 ± 2.59 b | 91.00 ± 1.73 a |
8 | 6.50 ± 0.03 b | 53.17 ± 3.03 b | 72.67 ± 1.76 c |
9 | 6.43 ± 0.09 b | 41.33 ± 1.09 c | 63.33 ± 2.33 d |
10 | 6.21 ± 0.04 c | 35.83 ± 3.91 cd | 56.33 ± 2.02 e |
Table 4 The effect of pH on mycelium growth,spore production and germination
pH | 菌落直径/cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/% Spore germination |
---|---|---|---|
4 | 3.80 ± 0.03 e | 33.33 ± 0.73 d | 69.67 ± 1.33 cd |
5 | 5.58 ± 0.03 d | 38.50 ± 0.87 cd | 79.33 ± 2.40 b |
6 | 5.67 ± 0.04 d | 64.83 ± 1.20 a | 79.67 ± 2.96 b |
7 | 6.87 ± 0.04 a | 54.33 ± 2.59 b | 91.00 ± 1.73 a |
8 | 6.50 ± 0.03 b | 53.17 ± 3.03 b | 72.67 ± 1.76 c |
9 | 6.43 ± 0.09 b | 41.33 ± 1.09 c | 63.33 ± 2.33 d |
10 | 6.21 ± 0.04 c | 35.83 ± 3.91 cd | 56.33 ± 2.02 e |
温度/ ℃ Temperature | 菌落直径/ cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/% Spore germination |
---|---|---|---|
5 | 0.58 ± 0.01 f | 7.50 ± 0.29 e | 29.33 ± 1.76 d |
10 | 0.58 ± 0.02 f | 3.33 ± 0.17 ef | 16.33 ± 1.76 e |
15 | 0.84 ± 0.07 e | 88.17 ± 2.20 a | 17.33 ± 0.33 e |
20 | 2.93 ± 0.02 d | 77.83 ± 1.97 b | 45.00 ± 1.53 c |
25 | 6.00 ± 0.09 b | 41.00 ± 1.32 d | 69.00 ± 1.73 b |
30 | 6.36 ± 0.05 a | 45.67 ± 1.01 c | 90.33 ± 2.02 a |
35 | 5.60 ± 0.03 c | 74.33 ± 2.59 b | 36.00 ± 5.03 d |
40 | 0.70 ± 0.02 f | 0 f | 19.67 ± 2.02 e |
Table 5 The effect of temperature on mycelium growth,spore production and germination
温度/ ℃ Temperature | 菌落直径/ cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/% Spore germination |
---|---|---|---|
5 | 0.58 ± 0.01 f | 7.50 ± 0.29 e | 29.33 ± 1.76 d |
10 | 0.58 ± 0.02 f | 3.33 ± 0.17 ef | 16.33 ± 1.76 e |
15 | 0.84 ± 0.07 e | 88.17 ± 2.20 a | 17.33 ± 0.33 e |
20 | 2.93 ± 0.02 d | 77.83 ± 1.97 b | 45.00 ± 1.53 c |
25 | 6.00 ± 0.09 b | 41.00 ± 1.32 d | 69.00 ± 1.73 b |
30 | 6.36 ± 0.05 a | 45.67 ± 1.01 c | 90.33 ± 2.02 a |
35 | 5.60 ± 0.03 c | 74.33 ± 2.59 b | 36.00 ± 5.03 d |
40 | 0.70 ± 0.02 f | 0 f | 19.67 ± 2.02 e |
光照 Light | 菌落直径/ cm Colony diameter | 产孢量/(×106 CFU · mL-1) Sporulation | 孢子萌发率/ % Spore germination |
---|---|---|---|
全光照 All light | 6.50 ± 0.08 a | 1.83 ± 0.17 c | 21.00 ± 2.52 c |
光照交替 Dark/ Light | 6.50 ± 0.08 a | 47.33 ± 1.76 b | 80.00 ± 1.53 b |
全黑暗 All dark | 5.88 ± 0.02 b | 139.83 ±1.48 a | 91.67 ± 0.09 a |
Table 6 The effect of light on mycelium growth,spore production and germination
光照 Light | 菌落直径/ cm Colony diameter | 产孢量/(×106 CFU · mL-1) Sporulation | 孢子萌发率/ % Spore germination |
---|---|---|---|
全光照 All light | 6.50 ± 0.08 a | 1.83 ± 0.17 c | 21.00 ± 2.52 c |
光照交替 Dark/ Light | 6.50 ± 0.08 a | 47.33 ± 1.76 b | 80.00 ± 1.53 b |
全黑暗 All dark | 5.88 ± 0.02 b | 139.83 ±1.48 a | 91.67 ± 0.09 a |
培养基 Media | 菌落直径/ cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/% Spore germination |
---|---|---|---|
WA | 2.92 ± 0.03 f | 2.67 ± 1.67 g | 80.67 ± 3.93 b |
PDA | 6.00 ± 0.09 a | 41.00 ± 1.32 d | 73.67 ± 1.45 bc |
PSA | 5.75 ± 0.02 b | 48.67 ± 1.01 c | 76.00 ± 5.03 b |
MEA | 5.31 ± 0.01 c | 60.83 ± 0.60 b | 65.00 ± 2.65 c |
CA | 5.02 ± 0.01 d | 33.00 ± 1.53 e | 91.00 ± 1.00 a |
OMA | 4.58 ± 0.09 e | 14.83 ± 1.20 f | 80.00 ± 3.61 b |
PCA | 4.92 ± 0.03 d | 73.83 ± 2.24 a | 91.67 ± 1.45 a |
Table 7 The effect of media on mycelium growth,spore production and germination
培养基 Media | 菌落直径/ cm Colony diameter | 产孢量/(× 106 CFU · mL-1) Sporulation | 孢子萌发率/% Spore germination |
---|---|---|---|
WA | 2.92 ± 0.03 f | 2.67 ± 1.67 g | 80.67 ± 3.93 b |
PDA | 6.00 ± 0.09 a | 41.00 ± 1.32 d | 73.67 ± 1.45 bc |
PSA | 5.75 ± 0.02 b | 48.67 ± 1.01 c | 76.00 ± 5.03 b |
MEA | 5.31 ± 0.01 c | 60.83 ± 0.60 b | 65.00 ± 2.65 c |
CA | 5.02 ± 0.01 d | 33.00 ± 1.53 e | 91.00 ± 1.00 a |
OMA | 4.58 ± 0.09 e | 14.83 ± 1.20 f | 80.00 ± 3.61 b |
PCA | 4.92 ± 0.03 d | 73.83 ± 2.24 a | 91.67 ± 1.45 a |
[1] | Chen Cun-kun, Wang Wen-sheng, Gao Yuan-hui, Feng Jiong-qi, Dong Cheng-hu, Wang Dong-li. 2008. Separation and identification of pathogenic fungi from postharvest thick-skinned melon. Storage and Process,(6):54-56. (in Chinese) |
陈存坤, 王文生, 高元惠, 冯炯琦, 董成虎, 王东礼. 2008. 新疆厚皮甜瓜采后病害及主要病原真菌的分离与鉴定. 保鲜与加工,(6):54-56. | |
[2] |
Daley J, Branham S, Levi A, Hassell R, Wechter P. 2017. Mapping resistance to Alternaria cucumerina in Cucumis melo. Phytopathology, 107 (4):427-432.
doi: 10.1094/PHYTO-06-16-0246-R URL |
[3] | Fang Zhong-da. 1998. Plant pathology research methods. Beijing: China Agriculture Press. (in Chinese) |
方中达. 1998. 植病研究方法. 北京: 中国农业出版社. | |
[4] | Feng Bao-zhen, Li Pei-qian. 2019. Identification of the pathogen causing black spot of Chinese rose and fungicide screening for the disease control. Journal of Plant Protection, 46 (5):1147-1154. (in Chinese) |
冯宝珍, 李培谦. 2019. 月季黑斑病病原菌鉴定及室内药剂初步筛选. 植物保护学报, 46 (5):1147-1154. | |
[5] | He Xiang, Sun Lei, Aierken Reheman, Abuduwaili Abudureyimu, Muheta Abudukelimu. 2010. Isolation and identification of black spot pathogens from winter storage melon. Xinjiang Agricultural Sciences, 47 (7):1365-1369,1268. (in Chinese) |
何香, 孙磊, 艾尔肯 · 热合曼, 阿不都外力 · 阿布都热依木, 木合塔 · 阿布都克里木. 2010. 冬季储藏甜瓜黑斑病病原菌的分离与鉴定. 新疆农业科学, 47 (7):1365-1369,1268. | |
[6] |
Lawrence D P, Rotondo F, Gannibal P B. 2016. Biodiversity and taxonomy of the pleomorphic genus Alternaria. Mycological Progress, 15 (3):1-22.
doi: 10.1007/s11557-015-1147-7 URL |
[7] | Lei Zao-juan, Wu Ya-ming, Liu Zi-huan, An Peng-tian, Dou Hua-yun, Meng Zhen-shan. 2020. Isolation and identification of Alternaria tenuissima from Huanghua winter jujube. China Plant Protection, 40 (4):17-20. (in Chinese) |
雷早娟, 武亚明, 刘子欢, 安鹏天, 窦华云, 孟震山. 2020. 黄骅冬枣细极链格孢的分离鉴定. 中国植保导刊, 40 (4):17-20. | |
[8] | Liu Qiao, Ning Nan-nan, Ma Yong-qiang, Guo Qing-yun. 2020. Isolation and identification of the pathogen causing leaf spot on cherry in Qinghai province. Plant Protection, 46 (2):48-55,71. (in Chinese) |
刘俏, 宁楠楠, 马永强, 郭青云. 2020. 青海省樱桃叶斑病病原菌的分离与鉴定. 植物保护, 46 (2):48-55,71. | |
[9] | Liu Xingfeng, Liu Dong, Tao Lei, Pan Chunqing, Li Xuelian, Ma Tian, Pan Mengjia, Zhang Yanju. 2021. Pathogen identification and biological characteristics of cucumber alternaria leaf spot in Heilongjiang Province. China Vegetables,(8):80-86. (in Chinese) |
刘行风, 刘东, 陶磊, 潘春清, 李雪莲, 马天, 潘梦佳, 张艳菊. 2021. 黑龙江省黄瓜链格孢叶斑病病原鉴定及生物学特性研究. 中国蔬菜,(8):80-86. | |
[10] | Liu Yu-feng, Zhang Zheng, Ma Yin-feng. 2013. Isolation and identification of the causal agent of Akebia trifoliate leaf spot disease. Plant Protection, 39 (2):57-62. (in Chinese) |
刘昱锋, 张铮, 马银峰. 2013. 三叶木通叶斑病病原菌鉴定. 植物保护, 39 (2):57-62. | |
[11] | Lu Yuan-yuan, Song Yan-bo, Qiao Yong-gang, Liu Zhen-yu. 2020. Pathogen identification of lycium leaf spot and its biological characteristics in Shanxi Province. Journal of Shanxi Agricultural University(Natural Science Edition), 40 (6):75-83. (in Chinese) |
路媛媛, 宋艳波, 李丽, 乔永刚, 刘振宇. 2020. 山西省枸杞叶斑病病原鉴定及其生物学特征. 山西农业大学学报(自然科学版), 40 (6):75-83. | |
[12] | Ma Guo-ping, Liang Zhi-jian, Hua Hui-hui, Wu Xue-hong. 2020. Studies on the identification and pathogenicity of Alternaria spp. associated with muskmelon seeds. Acta Phytopathologica Sinica, 50 (1):1-9. (in Chinese) |
马国苹, 梁芷健, 华晖晖, 吴学宏. 2020. 甜瓜种子携带的链格孢菌种类鉴定及其致病性研究. 植物病理学报, 50 (1):1-9. | |
[13] | Meng Ting-ting, Liu Chuang, Qi Ying-bo, Dai Han-ping, Liu Yan-ru, Yan Xue-rui. 2019. Identification of pathogen of raspberry stem canker and its biological characteristics. China Plant Protection, 39 (5):15-20. (in Chinese) |
孟婷婷, 刘闯, 齐鹰博, 代汉萍, 刘艳茹, 严雪瑞. 2019. 树莓茎溃疡病病原菌鉴定及其生物学特性研究. 中国植保导刊, 39 (5):15-20. | |
[14] |
Simmons E G. 1967. Typification of Alternaria,Stemphylium and Ulocladium. Mycologia, 59 (1):67-92.
pmid: 6068270 |
[15] |
Vakalounakis D J. 1990. Alternaria alternata f. sp. cucurbitae,the cause of a new leaf spot disease of melon(Cucumis melo). Annals of Applied Biology, 117 (3):507-513.
doi: 10.1111/aab.1990.117.issue-3 URL |
[16] | Wang Hong, Chang You-hong, Chen Zhi-yi. 2006. Study on biological characteristics of Alternaria alternata caused occurrence of pear black spot. Journal of Fruit Science, 23 (2):247-251. (in Chinese) |
王宏, 常有宏, 陈志谊. 2006. 梨黑斑病病原菌生物学特性研究. 果树学报, 23 (2):247-251. | |
[17] | Wang Yu-hong, Gao Tian-yi, Zhang Hua-feng, Huang Yun-ping, Yan Lei-yan, Xing Nai-lin, Wang Ying-er, Ying Quan-sheng. 2018. Comprehensive prevention and control technology for continuous cropping obstacles of western melon. China Vegetables,(11):81-84. (in Chinese) |
王毓洪, 高天一, 张华峰, 黄芸萍, 严蕾艳, 邢乃林, 王迎儿, 应泉盛. 2018. 设施西甜瓜连作障碍综合防控技术. 中国蔬菜,(11):81-84. | |
[18] | Wei Jing-chao. 1979. Fungal identification manual. Shanghai: Shanghai Scientific & Technical Publishers. (in Chinese) |
魏景超. 1979. 真菌鉴定手册. 上海: 上海科学技术出版社. | |
[19] | Wu Hui-jie, Kang Bao-shan, Peng Bin, Liu Li-feng, Gu Qin-sheng. 2020. Construction of yeast two-hybrid cDNA library in response to MNSV infection in melon. China Vegetables,(9):36-40. (in Chinese) |
吴会杰, 康保珊, 彭斌, 刘丽锋, 古勤生. 2020. 甜瓜响应甜瓜坏死斑点病毒侵染的cDNA文库构建. 中国蔬菜,(9):36-40. | |
[20] | Yuan Pu-ying, Zhu Tian-hui. 2008. Identification and biological characteristics of sweet osmanthus leaf blight. Journal of Sichuan Forestry Science and Technology,(4):21-28. (in Chinese) |
袁蒲英, 朱天辉. 2008. 桂花叶枯病病原菌鉴定及生物学特性研究. 四川林业科技,(4):21-28. | |
[21] | Zhang Qin, Jiang Chun-ming, Li Jin-long, Li Cheng-de. 2011. A new muskmelon F1 hybird-‘Yinguan’. China Vegetables,(14):102-105. (in Chinese) |
张勤, 蒋春明, 李锦龙, 李城德. 2011. 厚皮甜瓜新品种银冠的选育. 中国蔬菜,(14):102-105. | |
[22] | Zhang Qin, Jiang Chun-ming, Li Jin-long, Li Cheng-de. 2017. High-quality and high-efficiency cultivation techniques of large-fruited thick-skin melon interplanting sunflower. China Cucurbits and Vegetables, 30 (10):47-48. (in Chinese) |
张勤, 蒋春明, 李锦龙, 李城德. 2017. 大果型厚皮甜瓜套种向日葵优质高效栽培技术. 中国瓜菜, 30 (10):47-48. | |
[23] | Zhang Tian-yu. 2003. Chinese fungi(Vol.16):Alternaria. Beijing: Science Press:19-41. (in Chinese) |
张天宇. 2003. 中国真菌志. 16卷. 链格孢属. 北京: 科学出版社:19-41. | |
[24] | Zhang Zheng, Li Xiu-qing, Liu Yu-feng. 2015. Biological characteristics and biological control of leaf spot disease pathogen on Akebiatrifoliata. Journal of Shaanxi Normal University(Natural Science Edition), 43 (5):61-66. (in Chinese) |
张铮, 李秀青, 刘昱锋. 2015. 三叶木通叶斑病病原菌的生物学特性及生物防治. 陕西师范大学学报(自然科学版), 43 (5):61-66. | |
[25] | Zhao Juan, Wu Xue-hong. 2014. Research progress on muskmelon fungal leaf spot. China Plant Protection, 34 (11):24-27,14. (in Chinese) |
赵娟, 吴学宏. 2014. 甜瓜真菌性叶斑病研究进展. 中国植保导刊, 34 (11):24-27,14. | |
[26] | Zhou X G, Everts K L. 2008. First report of Alternaria alternata f. sp. cucurbitae causing Alternaria leaf spot of melon in the mid-Atlantic region of the United States. Plant Disease: 92 (4):652. |
[1] | JIA Jin, XU Yunlong, ZHOU Jiale, YANG Xiaofen, ZHONG Yonghui, ZENG Jiwu, HONG Ni, WANG Guoping, PENG Shu’ang, and DING Fang, . Detection and Identification of Huanglongbing Bacteria in‘Cocktail’Grapefruit [J]. Acta Horticulturae Sinica, 2022, 49(3): 590-596. |
[2] | JIANG Cuicui, FANG Zhizhen, ZHOU Danrong, LIN Yanjuan, YE Xinfu. Identification and Expression Analysis of Sugar Transporter Family Genes in‘Furongli’(Prunus salicina) [J]. Acta Horticulturae Sinica, 2022, 49(2): 252-264. |
[3] | XIE Siyi, ZHOU Chengzhe, ZHU Chen, ZHAN Dongmei, CHEN Lan, WU Zuchun, LAI Zhongxiong, GUO Yuqiong. Genome-wide Identification and Expression Analysis of CsTIFY Transcription Factor Family Under Abiotic Stress and Hormone Treatments in Camellia sinensis [J]. Acta Horticulturae Sinica, 2022, 49(1): 100-116. |
[4] | YU Lin, SHE Xiaoman, TANG Yafei, LAN Guobing, LI Zhenggang, HE Zifu. Identification of Pathogen Causing Allium ascalonicum Anthracnose in Guangdong [J]. Acta Horticulturae Sinica, 2022, 49(1): 129-140. |
[5] | ZHANG Chunyu, XU Xiaoqiong, XU Xiaoping, ZHAO Pengcheng, SHEN Xu, Munir Nigarish, ZHANG Zihao, LIN Yuling, Chen Zhenguang, LAI Zhongxiong. Genome-wide Identification of the SKP1-like Family and Analysis of Their Expression During Early Somatic Embryogenesis in Longan [J]. Acta Horticulturae Sinica, 2021, 48(9): 1665-1679. |
[6] | SHI Liting, ZHOU Xinyang, YE Jianfeng, ZHOU Jiahao, WANG Gang, XIA Guohua. Advances in Distant Hybridization Breeding of Woody Ornamental Plants [J]. Acta Horticulturae Sinica, 2021, 48(9): 1827-1838. |
[7] | LI Na, SHANG Jianli, LI Nannan, ZHOU Dan, KONG Shengnan, WANG Jiming, MA Shuangwu. Accurate Molecular Identification for Fruit Shape in Watermelon (Citrullus lanatus) [J]. Acta Horticulturae Sinica, 2021, 48(7): 1386-1396. |
[8] | CAI Roudi, LI Xue, CHEN Yan, XU Xiaoping, CHEN Xiaohui, LAI Zhongxiong, LIN Yuling. Genome-wide Identification and Expression Analysis of DRB Gene Family in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2021, 48(5): 921-933. |
[9] | HU Jingtao, RUAN Yu, GAN Liping. Identification and Expression Analysis of the B-box Transcription Factor Family in Pepper [J]. Acta Horticulturae Sinica, 2021, 48(5): 987-1001. |
[10] | XIAO Xiao, DING Liang, CONG Yu, XU Xuling, QIAO Huan, HE Silong, XU Wenjian, XU Tianshun. Isolation and Identification of a Lytic Bacteriophage Infecting Xanthomonas axonopodis pv. citri [J]. Acta Horticulturae Sinica, 2021, 48(12): 2349-2359. |
[11] | JIANG Mengqi, XUE Xiaodong, SU Liyao, CHEN Yan, ZHANG Shuting, LI Xiaofei, WANG Peiyu, ZHANG Zihao, LAI Zhongxiong, LIN Yuling. Genome-wide Identification and Expression Analysis of TCP Family in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2021, 48(12): 2481-2496. |
[12] | ZHANG Qian, YANG Nan, SANG Haiyu, ZHAO Rong, SONG Xiaoxi, CHEN Longqing, XIANG Lin, ZHAO Kaige. Cloning and Functional Analysis of CpTT8 Related to Anthocyanin Synthesis in Wintersweet(Chimonanthus praecox) [J]. Acta Horticulturae Sinica, 2021, 48(10): 1945-1955. |
[13] | LI Xinxin, HOU Hongmin, XU Jihua, SUN Xiaohong, ZHANG Yugang. Genome-wide Identification and Abiotic Stress Response Analysis of MLP Family Genes in Apple [J]. Acta Horticulturae Sinica, 2021, 48(1): 1-14. |
[14] | ZHANG Zhongxing, CHENG Li, WANG Shuangcheng, ZHANG De, LIU Bing, WANG Yanxiu. Cloning of MhMYB114-Like from Malus halliana and Its Functional Identification of Iron Deficiency Tolerance in Apple Callus [J]. Acta Horticulturae Sinica, 2021, 48(1): 127-136. |
[15] | ZHAO Hua1,ZHANG Shuting1,LIU Pudong1,FU Shuai1,YE Wei2,CHEN Yukun1,LIN Yuling1,XU Han1,and LAI Zhongxiong1,*. Genome-wide Identification and Expression Analysis of JmjC Gene Family in Response to Different Photoperiods in Dendrobium [J]. ACTA HORTICULTURAE SINICA, 2020, 47(8): 1505-1520. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Copyright © 2012 Acta Horticulturae Sinica 京ICP备10030308号-2 国际联网备案号 11010802023439
Tel: 010-82109523 E-Mail: yuanyixuebao@126.com
Support by: Beijing Magtech Co.Ltd