Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 427-436.doi: 10.16420/j.issn.0513-353x.2020-0929

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Isolation and Identification of Melon Black Spot in Gansu Province

SHI Zhaorong1, ZHANG Guangrong2, SUN Shujun2, JIN Mengjun1, CUI Lingxiao1, LIU Yanchao1, WEI Lijuan1, MEI Dahai1, YANG Chengde1,*()   

  1. 1College of Plant Protection,Gansu Agricultural University,Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,China
    2The Agri-Tech Extension Service Center of Baiyin City,Baiyin,Gansu 730900,China
  • Received:2021-04-21 Revised:2021-08-27 Online:2022-02-25 Published:2022-02-28
  • Contact: YANG Chengde


This experiment was conducted to isolate,identify and determine the biological characteristics of black spot pathogen on melon in Baiyin City,Gansu Province. The results showed that the colony of isolate 98BYT2-1 was green-brown,round,conidia solitary or clustered,inverted rod-shaped or oval,with a vertical and horizontal septum. The size of conidia was(21.94-52.10)μm ×(7.01-13.93)μm,combining ITS,CAL and gpd gene sequence analysis showed that the strain 98BYT2-1 had 100% homology with Alternaria tenuissima and they were clustered together on the phylogenetic tree. Together,the isolate was identified as A. tenuissima. Starch,lactose and glucose were the most appropriate carbon sources for mycelia growth,spore formation and spore germination,respectively. Yeast extract and beef extract were the most suitable nitrogen sources for mycelia growth,but peptone was the most suitable source of nitrogen for sporulation and spore germination. In addition,PDA facilitated the mycelia growth of pathogen,and PCA was suitable for sporulation and spore germination. The optimum pH for mycelia growth and spore germination was 7 and the value for sporulation was 6. The optimum temperature for the growth and spore germination of mycelia was 30 ℃,and the sporulation was 15 ℃. The dark held up the growth of mycelium but benefited to sporulation and spore germination. The results obtained will provide a basis for the diagnosis and comprehensive control of melon black spot in Gansu Province.

Key words: muskmelon alternaria black spot, Alternaria tenuissima, identification, biological characteristics, determination

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