Abstract: Abstract：In order to provide tool of biology research and genetic improvement for Taxus wallichiana
var. mairei，SSR molecular markers were developed with GSS sequence in this study. In total 184 SSR loci
were find from 1 923 GSS sequences. Among those loci，the proportion of two nucleotide repeats，three
nucleotide repeats and four nucleotide repeats are 88.6%，10.3%，1.1%，respectively. The frequency of a
and t are higher than that of c and g in the nucleotide repeats in the T. wallichiana var. mairei genome.
From those 184 SSR loci，90 SSR candidate primers were exploited. In order to verify the validity of
candidate primers，20 primers were selected for further test. PCR result showed that 19 can gain clear
products. Those results showed that GSS data could be employed to exploit SSR marker. Those markers
can be well used in T. wallichiana var. mairei research.

Key words: Taxus wallichiana var. mairei, GSS sequence, SSR, frequency