Abstract: With coleus（Coleus blumei Benth.）for the materials，using the planting method with
nutrient solution containing 1.0 mmol · L^-1 Pb ²⁺ and adding different concentrations（0.1，0.5，1.0，2.5，
5.0 mg · L^-1）sodium selenite，from the spectroscopy to study the effect of different selenium（Se）
concentrations on the morphological and structural changes of Coleus under lead（Pb）stress by X-ray
diffraction（XRD）and scanning electron microscope（SEM）analysis. The results showed that the particle
microstructure in the roots and leaves of Coleus under Pb stress were great influenced by different Se
concentrations，the particle distribution in the roots and leaves from the SEM image of 1.0 mg · L^-1 Se
treatment was more uniform than the other concentration，and structure closer and flat plait shape，XRD
diffraction angle moved to the right，and strength，high peak，peak area，and FWHM reduced. These
indicated that 1.0 mg · L^-1 Se can reduce the migration and transformation of Pb in the solution，and
explain the changes of combination form of Pb in the Coleus through the particle distribution，diffraction
angle remove，the changes of strength，high peak，peak area and FWHM.

Key words: Coleus blumei, selenium, lead, X-ray diffraction（XRD）, scanning electron microscopy
（SEM）, morphological and structural