Genetic Analysis and Development of Associated SSR Markers of the Flower Color in F1 Population of Hedychium coronarium‘COR01’× H. ‘Jin’

doi:10.16420/j.issn.0513-353x.2021-0624

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (10): 1921-1933.doi: 10.16420/j.issn.0513-353x.2021-0624

• Research Papers • Previous Articles Next Articles

Genetic Analysis and Development of Associated SSR Markers of the Flower Color in F₁ Population of Hedychium coronarium‘COR01’× H. ‘Jin’

ZHOU Yiwei¹^,², XU Guoyu¹^,², WANG Qin¹^,², YAN Fulong¹^,², YU Yunyi¹^,², YU Rangcai²^,³, FAN Yanping¹^,²^,^**()

¹College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China
²Flower Research Center Guangzhou 510642,China
³College of Life Science,South China Agricultural University,Guangzhou 510642,China

Received:2021-08-06 Revised:2021-09-10 Online:2021-10-25 Published:2021-11-01
Contact: FAN Yanping E-mail:fanyanping@scau.edu.cn

Abstract

Abstract:

In this study,114 F₁ hybrids derived from the cross between Hedychium coronarium‘COR01’and H.‘Jin’were used as materials. Four important flower color-related traits including the dominant color of labellum,labellum heart,filament,and anther were observed and analysed. Meanwhile,bulked segregant analysis（BSA）was used to develop color-related molecular markers. The results showed that the flower color of 114 F₁ hybrids was widely separated,and the Shannon-Wiener index of the main color of labellum petal,labellum heart,filament,and anther ranged from 0.57 to 1.58. Colorimeter analysis indicated that the L^*,a^* and b^*values of the dominant color of labellum and labellum heart were continuously distributed. Cluster analysis revealed that 114 F₁ hybrids could be divided into white group and orange group. BSA-SSR analysis confirmed that the HcgSSR676 marker screened out from 191 primers pairs was closely related to flower color traits. The marker was significantly associated with the white/orange group,yellowness value of labellum dominant color（b^*₁）,brightness value（L^*₂）,redness value（a^*₂）,and yellowness value（b^*₂）of labellum heart dominant color,whose regression determination coefficient（R²）ranged from 7.92 to 23.19.

Key words: Hedychium, flower color, genetic analysis, SSR, BSA

CLC Number:

S682

ZHOU Yiwei, XU Guoyu, WANG Qin, YAN Fulong, YU Yunyi, YU Rangcai, FAN Yanping. Genetic Analysis and Development of Associated SSR Markers of the Flower Color in F₁ Population of Hedychium coronarium‘COR01’× H. ‘Jin’[J]. Acta Horticulturae Sinica, 2021, 48(10): 1921-1933.

Figures/Tables 11

References 43

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引物编号 Primer number	SSR基序 SSR motif	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
HcgSSR119	（TTGCCA）₇	TTACTGCGACCACAGCAAAC	TTGATTTACTCGCTTCCGCT
HcgSSR649	（AAAATT）₆	GGATTGAAGTTAGAAGTTGATATTGGA	TGTGGTTTGAGATTAGGGGTG
HcgSSR676	（AT）₂₇	CTTGTTGATTCCACTTCCAAAA	ACCACCTCAGCAGGTACACA
HcgSSR926	（TA）₄₁	TCGCACATATAAGGGTGTGC	CCCAATCAATTTGAAAACCAA

引物编号 Primer number	SSR基序 SSR motif	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
HcgSSR119	（TTGCCA）₇	TTACTGCGACCACAGCAAAC	TTGATTTACTCGCTTCCGCT
HcgSSR649	（AAAATT）₆	GGATTGAAGTTAGAAGTTGATATTGGA	TGTGGTTTGAGATTAGGGGTG
HcgSSR676	（AT）₂₇	CTTGTTGATTCCACTTCCAAAA	ACCACCTCAGCAGGTACACA
HcgSSR926	（TA）₄₁	TCGCACATATAAGGGTGTGC	CCCAATCAATTTGAAAACCAA

观测部位 Observation position	姜花 Hedychium	比色卡 RHS	颜色 Color	数量（占比%） Count	H’ 香农—维纳指数 Shannon-Wiener index
唇瓣Labellum	♀	155D	白White
	♂	19C	橙黄Orange-yellow
	F₁	155B ~ NN155D	白White	95（83.33）	0.57
		158C ~ 158D	黄白Yellow-white	2（1.75）
		159A ~ 159D	橙白Orange-white	15（13.16）
		11D	黄Yellow	1（0.88）
		27A	橙Orange	1（0.88）
唇瓣心部	♀	2D	黄Yellow
Labellum heart	♂	28A	橙Orange
	F₁	145D ~ 150D	黄绿Yellow-green	42（39.47）	1.52
		1D ~ 13A	黄Yellow	14（12.28）
		15A ~ 23C	橙黄Orange-yellow	23（20.18）
		24A ~ 28B	橙Orange	20（17.54）
		30D ~ 35B	橙红Orange-red	15（13.16）
花丝Filament	♀	155B	白White
	♂	22B	橙黄Orange-yellow
	F₁	155A ~ NN155D	白White	27（23.68）	1.58
		4D ~ 14D	黄Yellow	14（12.28）
		14D ~ 23C	橙黄Orange-yellow	28（24.56）
		24A ~ 28D	橙Orange	21（18.42）
		30B ~ 35B	橙红Orange-red	24（21.05）
花药 Anther	♀	4D	白White
	♂	22B	橙黄Orange-yellow
	F₁	4D ~ 11D	黄Yellow	24（21.05）	1.34
		14D ~ 24C	橙黄Orange-yellow	35（30.70）
		24A ~ 28B	橙Orange	38（33.33）
		30A ~ 33D	橙红Orange-red	17（14.91）

观测部位 Observation position	姜花 Hedychium	比色卡 RHS	颜色 Color	数量（占比%） Count	H’ 香农—维纳指数 Shannon-Wiener index
唇瓣Labellum	♀	155D	白White
	♂	19C	橙黄Orange-yellow
	F₁	155B ~ NN155D	白White	95（83.33）	0.57
		158C ~ 158D	黄白Yellow-white	2（1.75）
		159A ~ 159D	橙白Orange-white	15（13.16）
		11D	黄Yellow	1（0.88）
		27A	橙Orange	1（0.88）
唇瓣心部	♀	2D	黄Yellow
Labellum heart	♂	28A	橙Orange
	F₁	145D ~ 150D	黄绿Yellow-green	42（39.47）	1.52
		1D ~ 13A	黄Yellow	14（12.28）
		15A ~ 23C	橙黄Orange-yellow	23（20.18）
		24A ~ 28B	橙Orange	20（17.54）
		30D ~ 35B	橙红Orange-red	15（13.16）
花丝Filament	♀	155B	白White
	♂	22B	橙黄Orange-yellow
	F₁	155A ~ NN155D	白White	27（23.68）	1.58
		4D ~ 14D	黄Yellow	14（12.28）
		14D ~ 23C	橙黄Orange-yellow	28（24.56）
		24A ~ 28D	橙Orange	21（18.42）
		30B ~ 35B	橙红Orange-red	24（21.05）
花药 Anther	♀	4D	白White
	♂	22B	橙黄Orange-yellow
	F₁	4D ~ 11D	黄Yellow	24（21.05）	1.34
		14D ~ 24C	橙黄Orange-yellow	35（30.70）
		24A ~ 28B	橙Orange	38（33.33）
		30A ~ 33D	橙红Orange-red	17（14.91）

观测部位 Observation site	指标 Colorimeter	白色系White group		橙色系Orange group		t统计值 t-statistic	P值 P-value
观测部位 Observation site	指标 Colorimeter	范围Range	均值Mean	范围Range	均值Mean	t统计值 t-statistic	P值 P-value
唇瓣Labellum	L^*₁	86.38 ~ 96.56	91.34	78.25 ~ 93.97	89.07	-4.54	1.42E-05^**
	a^*₁	-0.05 ~ 2.98	0.96	-0.50 ~ 12.30	1.70	2.11	0.037^*
	b^*₁	-2.88 ~ 9.99	1.44	-0.58 ~ 31.31	11.67	9.66	1.97E-16^**
唇瓣心部 Labellum heart	L^*₂	82.09 ~ 90.49	86.75	55.16 ~ 83.71	73.11	-14.10	1.43E-26^**
	a^*₂	-2.03 ~ 2.97	-0.22	0.79 ~ 47.55	16.47	10.72	7.11E-19^**
	b^*₂	2.96 ~ 22.79	10.85	19.56 ~ 62.43	41.60	20.05	7.48E-39^**

Genetic Analysis and Development of Associated SSR Markers of the Flower Color in F₁ Population of Hedychium coronarium‘COR01’× H. ‘Jin’

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性状Trait		相关系数Correlation coefficient	R²
白色系/橙色系 White group/orange group		-0.47^**	21.56
唇瓣Labellum	L^*₁	0.22^*	3.81
	a^*₁	-0.11	3.62
	b^*₁	-0.33^**	10.09
唇瓣心部 Labellum heart	L^*₂	0.34^**	10.69
	a^*₂	-0.30^**	7.92
	b^*₂	-0.49^**	23.19

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[3]	SHEN Zhiguo, ZHANG Lin, YUAN Deyi, CHENG Jianming. Research Progress on Flower Color and New Red Flower Resource of Wintersweets（Chimonanthus praecox） [J]. Acta Horticulturae Sinica, 2022, 49(4): 924-934.
[4]	WANG Jing, XU Leifeng, WANG Ling, QI Xianyu, SONG Meng, CAO Yuwei, HE Guoren, TANG Yuchao, YANG Panpan, MING Jun. The Numerical Classification of Flower Color Phenotype in Lily [J]. Acta Horticulturae Sinica, 2022, 49(3): 571-580.
[5]	LI Chao, YANG Ying, CHEN Wei, ZHENG Heyun, LIAO Xinfu, SUN Yuping. Construction of DNA Fingerprinting and Clustering Analysis with SSR Markers for the Muskmelon of Xizhoumi Series [J]. Acta Horticulturae Sinica, 2022, 49(3): 622-632.
[6]	TANG Chenqian, QIU Zhixin, TAN Chao, QIAN Yuming, CHEN Xin. Sorbus koehneana（Rosaceae）：Its Complete Chloroplast Genome and Phylogenetic Relationship with S. unguiculata [J]. Acta Horticulturae Sinica, 2022, 49(3): 641-654.
[7]	ZHOU Lin, ZOU Hongzhu, HAN Lulu, JIA Yinghua, WANG Yan. Research Progress on the Role of Glycosyltransferases in Color Formation of Petals [J]. Acta Horticulturae Sinica, 2022, 49(3): 687-700.
[8]	LU Tiantian, LIU Zhiyuan, XU Zhaosheng, ZHANG Helong, LI Guoliang, SHE Hongbing, QIAN Wei. Genome-wide Association Studies for Flower Color in Common Bean [J]. Acta Horticulturae Sinica, 2022, 49(2): 332-340.
[9]	DENG Jiao, SU Mengyue, LIU Xuelian, OU Kefang, HU Zhengrong, YANG Pingfang. Transcriptome Analysis Revealed the Formation Mechanism of Floral Color of Lotus‘Dasajin’with Bicolor Petal [J]. Acta Horticulturae Sinica, 2022, 49(2): 365-377.
[10]	FANG Tian, LIU Jihong. Advances in Identification of QTLs Associated with Significant Traits in Major Fruit Trees [J]. Acta Horticulturae Sinica, 2022, 49(12): 2622-2640.
[11]	NIE Xinghua, LI Yiran, TIAN Shoule, WANG Xuefeng, SU Shuchai, CAO Qingqin, XING Yu, QIN Ling. Construction of DNA Fingerprint Map and Analysis of Genetic Diversity for Chinese Chestnut Cultivars（Lines） [J]. Acta Horticulturae Sinica, 2022, 49(11): 2313-2324.
[12]	CHEN Sijia, WANG Huan, LI Ruirui, WANG Zhuoyi, LUO Jing, WANG Caiyun. Characterization of CmMYC2 in Formation of Green Color in Ray Florets of Chrysanthemum [J]. Acta Horticulturae Sinica, 2022, 49(11): 2377-2387.
[13]	SUN Wei, SUN Shiyu, CHEN Yiran, WANG Yuhan, ZHANG Yan, JU Zhigang, YI Yin. Cloning and Function Analysis of Chalcone Isomerase Gene RdCHI1 in Rhododendron delavayi [J]. Acta Horticulturae Sinica, 2022, 49(11): 2407-2418.
[14]	GUO Xin, CHENG Fangyun, ZHONG Yuan, CHENG Xinyun, TAO Xiwen. The Quantitative Classification of Flower Color Phenotype in Paeonia rockii（Flare Tree Peony） [J]. Acta Horticulturae Sinica, 2022, 49(1): 86-99.
[15]	FENG Lixiao, HU Rong, BU Shan, ZHANG Deyong, LUO Xiangwen, LI Fan, DING Ming, ZHANG Zhuo, ZHANG Songbai, LIU Yong. Molecular Detection and Genetic Evolution Analysis of Yunnan Isolates of Lettuce Chlorosis Virus in Tomato [J]. Acta Horticulturae Sinica, 2022, 49(1): 141-147.