Abstract: Flat nectarine with excellent fruit quality was one of the important objectives of peach breeding currently. A progeny with 115 individuals from a cross of nectarine and flat nectarine（Xiaguang  NF）was used as plant materials to construct genetic linkage map and to scan QTLs controlling fruit flavor. Sugars and organic acids，including sucrose，glucose，sorbitol，fructose，malic acid，citric acid and quinic acid，were determined by HPLC. A total of 213 SSR and SRAP loci，which fit the 1:1 Mendelian expectation，were selected for the construction of linkage map by MapManager QTX20b. By comparing to SSR loci from T  E reference map of Prunus，eight linkage groups were found to be matched well. A 1 232.7 cM linkage map was formed with an average distance of 9.34 cM between two loci，and the map contains 22 SSR markers，108 SRAP markers and two quality traits，flat shape（S）and non acid（D）. Through interval mapping，28，17，and 10 QTLs were found when LOD≥2，LOD≥3，and LOD≥5，respectively. QTLs controlling fruit flavor were located on Group 1，Group 2，Group 4，Group 5 and Group 6. QTLs in Goup 1（Glu2，Sor2，Tsug4 and SSC3），Group 2（Glu4，Sor3 and SSC4）and Group 4（Suc2，Glu3，Glu5，Tsug5 and Taci4）were all showed to have a negative adding effects to their corresponding traits. Six QTLs located in Group 5（Suc1，Suc3，Tsug3，Mal1，Taci3，and Taci5）which were mainly around the non-acid loci（D），showed a positive adding effects to sugar，and a negative adding effects to organic acid. Nine QTLs were found on Group 6（Glu1，Fru1，Sor1，Tsug1，Tsug2，Qui1，Taci1，Taci2，SSC1 and SSC2），and all of them performed to have a positive adding effects to their corresponding traits.

Key words: Prunus persica, SSR, SRAP, linkage map, sugars, organic acid, QTLs