Optimization of AFLP System for Fuji and Identification Early-maturing Sport from ‘Changfu 2’

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (3): 327-332.

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Optimization of AFLP System for Fuji and Identification Early-maturing Sport from ‘Changfu 2’

LI Yuan-jun^1*,TANG Mei-ling^1*,YU Qing¹, LIU Mei-ying¹,SONG Lai-qing¹, and HAN Zhen-hai^2**

(¹Fruit Institute of Yantai Agricultural Science Academy, Yantai, Shandong 265500,China; ²Institute of Horticultural Plants，China Agricultural University，Beijing 100094，China)

Received:2008-10-17 Revised:2009-02-20 Online:2009-03-25 Published:2009-03-25
Contact: LI Yuan-jun;TANG Mei-ling;HAN Zhen-hai

Abstract

Abstract: Using AFLP to compare the early-maturing sport and its mother plant in ‘Changfu 2’．Sixty-four selective primer pairs was adopted in AFLP analysis. 2,700 clear bands was detected by 43 pairs of AFLP primers. There were 7 obvious bands difference between early-maturing sport and mother plant under 4 pair primers. The molecular weight range of the polymorphic fragments was mainly 350~800 bp．The results indicated that there were some molecular-level differentiation between 'Changfu 2' and its early-maturing sport. The application of AFLP in the research of apple bud mutation is Successfu1.

Key words: apple, AFLP, bud mutation

CLC Number:

S 661.1

LI Yuan-jun;TANG Mei-ling;YU Qing;LIU Mei-ying;SONG Lai-qing;and HAN Zhen-hai. Optimization of AFLP System for Fuji and Identification Early-maturing Sport from ‘Changfu 2’[J]. ACTA HORTICULTURAE SINICA, 2009, 36(3): 327-332.

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Optimization of AFLP System for Fuji and Identification Early-maturing Sport from ‘Changfu 2’

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