Primary Study on Identification of S-RNase Genes in Wild Myrobalan Plum (Prunus cerasifera Ehrh.)

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (3): 333-340.

• 果树 • Previous Articles Next Articles

Primary Study on Identification of S-RNase Genes in Wild Myrobalan Plum (Prunus cerasifera Ehrh.)

LIU Chong-qi¹,CHEN Xiao-liu¹,ZHANG Yan-min¹,LIN Qun¹, WANG Jin-zheng², ZHANG hong¹, ZHANG Chun-yu¹,and CHEN Xue-sen¹﹡

（¹State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an Shandong271018, China；²Shandong Institute of Pomology, Tai'an 271000, China）

Received:2008-11-18 Revised:2009-02-18 Online:2009-03-25 Published:2009-03-25
Contact: CHEN Xue-sen

Abstract

Abstract: On fourty-five strains of wild myrobalan plum, the PCR amplification was carried out with the primer pair PruC2/PruC4R designed according to the conserved sequence of amino acid of Prunus S-RNase. The PCR bands were cloned and sequenced. The sequences were blasted in GenBank which showed them having the maximum identity with Prunus S-Rnases, and four S-RNase alleles were identified and named as S₁ (511 bp), S₂(787 bp), S₃(1,859 bp) and S₄(464 bp) alleles respectively, their accession numbers in GenBank are EF638726, EF641276, EF661873 and EF661874 . The S-genotypes of 43 strains were S₁S₂, S₁S₃, S₂S₃, S₂S₄ and S₃S₄. However, only one S-RNase allele was identified as S3-RNase on strain 10 and S4-RNase on strain 15, the S-genotypes of strain 10 and 15 remain to be further studied.

Key words: Wild myrobalan plum（Prunus cerasifera Ehrh.）, Self-incompatibility, S-RNase, S-genotype

CLC Number:

S 662

LIU Chong-qi;CHEN Xiao-liu;ZHANG Yan-min;LIN Qun;WANG Jin-zheng;ZHANG hong;ZHANG Chun-yu;and CHEN Xue-sen﹡ . Primary Study on Identification of S-RNase Genes in Wild Myrobalan Plum (Prunus cerasifera Ehrh.)[J]. ACTA HORTICULTURAE SINICA, 2009, 36(3): 333-340.

[1]	WEI Zhuangmin, WEI Sijia, CHEN Peng, HU Jianbing, TANG Yuqing, YE Junli, LI Xianxin, DENG Xiuxin, CHAI Lijun. Identification of S-genotypes of 63 Pummelo Germplasm Resources [J]. Acta Horticulturae Sinica, 2022, 49(5): 1111-1120.
[2]	HE Min, GU Chao, WU Juyou, ZHANG Shaoling. Recent Advances on Self-incompatibility Mechanism in Fruit Trees [J]. Acta Horticulturae Sinica, 2021, 48(4): 759-777.
[3]	LI Guihua1，ZHANG Tianyu2，TANG Xiaobing3，CHEN Hancai1,*，LI Tingyao1，and ZHANG Yan1 . A New Chinese Kale Cultivar‘Zitai Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2777-2778.
[4]	LI Guihua1，CHEN Hancai1,*，ZHANG Yan1，LI Tingyao1，and CHEN Muxi2. A New Chinese Kale Cultivar‘Dongqiang Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2779-2780.
[5]	GAO Qiguo1,，LEI Zhenze1,，LIANG Yunfei1,*，JIANG Yupeng1，and ZHU Liquan2. Screening of Brassica oleracea MLPK-interacting PUB Proteins Based on Gene Family Analysis [J]. ACTA HORTICULTURAE SINICA, 2019, 46(4): 714-722.
[6]	YANG Dan1,3,，LI Qing1,，ZHU Liquan3，ZHAO Tiantian1,2，LIANG Lisong1,2，WANG Guixi1,2，and MA Qinghua1,2,**. Cloning and Expression Analysis of Calmodulin Gene in Ping’ou Hybrid Hazelnut（Corylus heterophylla × C. avellana） [J]. ACTA HORTICULTURAE SINICA, 2018, 45(7): 1347-1358.
[7]	DING Tiyu1,，WU Mengmeng1,，ZHANG Ruiping1,，YAN Zhenli1,，YAN Yaru2，CHEN Dixin2，ZHANG Hengtao1，and GAO Qiming1. Identification of S-genotypes of 20 Apple Cultivars [J]. ACTA HORTICULTURAE SINICA, 2018, 45(12): 2277-2290.
[8]	PU Min1，LIAN Xiaoping2,，LUO Shaolan1，ZHANG Hecui1，WANG Yukui1，BAI Xiaojing1，ZENG Jing1，GAO Qiguo2，REN Xuesong2，and ZHU Liquan1,*. Transcriptome Analysis of Stigma After Pollination in Brassica oleracea var. capitata [J]. ACTA HORTICULTURAE SINICA, 2018, 45(1): 71-84.
[9]	LI Guihua，CHEN Hancai*，ZHANG Yan，and LI Tingyao. A New Chinese Cabbage Cultivar‘Qiuguan’ [J]. ACTA HORTICULTURAE SINICA, 2017, 44(s2): 2673-2674.
[10]	LI Xiaoyu1，WANG Qiuhong2，LI Yuhua1，and LAN Xingguo1,*. Preparation of Monoclonal Antibodies Against BoARC1 and Expression Analysis of BoARC1 in Ornamental Kale [J]. ACTA HORTICULTURAE SINICA, 2017, 44(2): 355-363.
[11]	LI Gui-hua1，CHEN Han-cai1,*，LIU Kai2，ZHANG Yan1，and LI Ting-yao1. A New Chinese Kale Cultivar‘Qiulü Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2015, 42(S2): 2895-2896.
[12]	LI Yang, LI Chang-Long, WANG Jing, YAN Guo-Hua, ZHANG Xiao-Ming, LI Wei, ZHANG Kai-Chun, LI Tian-Zhong. Research of Relationship Between Sweet Cherry Lapins Self-compatibility and SFB4′ Gene [J]. ACTA HORTICULTURAE SINICA, 2015, 42(7): 1251-1259.
[13]	CAO Ming-ming，YANG Jia，LI Xiao-yu，LI Yu-hua，and LAN Xing-guo. Analysis and Characterization of Interaction Domain Between ARC1 and Exo70A1 from Ornamental Kale（Brassica oleracea var. acephala） [J]. ACTA HORTICULTURAE SINICA, 2015, 42(4): 791-798.
[14]	HE Shao-min，LI Chun-yu，LAN Cai-yun，ZOU Min，REN Xue-song，SI Jun，LI Cheng-qiong，and SONG Hong-yuan. Effect of Antisense RNA of the MLPK Gene on Self-incompatibility in Cabbage [J]. ACTA HORTICULTURAE SINICA, 2015, 42(2): 252-262.
[15]	JIANG Nan1,2,*，TAN Xiao-feng2，ZHANG Lin2，ZHANG Jing-guo3，and HU Hong-ju3. Preparation of S-RNase cDNA Microarray and Its Application in Identifying Pear Cultivars S-genotypes [J]. ACTA HORTICULTURAE SINICA, 2015, 42(12): 2341-2352.

Primary Study on Identification of S-RNase Genes in Wild Myrobalan Plum (Prunus cerasifera Ehrh.)

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments