Molecular Cloning and Expression of Sesquiterpenoid Synthase Gene in Hedychium coronarium Koenig

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (10): 1527-1532.

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Molecular Cloning and Expression of Sesquiterpenoid Synthase Gene in Hedychium coronarium Koenig

LI Rui-hong¹;FAN Yan-ping¹*;YU Rang-cai²;LU Wang-jin¹;and ZHUANG Chu-xiong2

(¹College of Horticulture，²College of Life Science, South China Agricultural University，Guangzhou 510642， China)

Received:2008-03-14 Revised:2008-08-26 Online:2008-10-25 Published:2008-10-25
Contact: FAN Yan-ping

Abstract

Abstract: A TPS gene named as Hc-Sesqui was isolated from H.coronarium leaf. The complete sequence of the cDNA gene was 1 932 bp, with an ORF encoding 551 amino acids. The amino acids sequence shared highly homology to other sesquiterpenoid synthase, containing conserved boxes of: TPS DDXXD. The phylogenetic analysis after Clustal.X alignment suggested that the Hc-Sesqui belonged to Tps-a. Northern blot revealed that the Hc-Sesqui gene was expressed in leaf, stem and sepal.

Key words: Hedychium coronarium Koenig, sesquiterpenoid synthase, gene cloning, expression

CLC Number:

S 68

LI Rui-hong;FAN Yan-ping;YU Rang-cai;LU Wang-jin;and ZHUANG Chu-xiong . Molecular Cloning and Expression of Sesquiterpenoid Synthase Gene in Hedychium coronarium Koenig[J]. ACTA HORTICULTURAE SINICA, 2008, 35(10): 1527-1532.

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Molecular Cloning and Expression of Sesquiterpenoid Synthase Gene in Hedychium coronarium Koenig

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