Obtaining of Ponkan (Citrus reticulata Blanco ) Tetraploid by Culturing Embryos of Mature Seeds

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (4): 688-690.

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Obtaining of Ponkan (Citrus reticulata Blanco ) Tetraploid by Culturing Embryos of Mature Seeds

Hong Liu;Liu Yongzhong;Deng Xiuxin

(National Key Laboratory of Crop Genetic Improvement, National Center of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, China)

Received:2004-10-21 Revised:2005-01-21 Online:2005-08-25 Published:2005-08-25

Abstract

Abstract: Seedless or few seed trait is one of the goals of Ponkan breeding. By culturing the embryos of mature seeds, a total of 449 polyembryonic seedlings from 374 seeds were obtained; and seven of them were verified to be tetrap loid by flow cytometry. SSR (simple sequence repeat) analyseswith 13 p rimers indicated
that these tetraploid seedlingswere all autotetrap loid from the nucellar cells. These tetraploid seedlings will be of great value for creating seedless Ponkan through sexual hybridization.

Key words: Ponkan, Citrus reticulata Blanco, Polyploidy, Tetraploid, Flow cytometry, SSR

CLC Number:

S666

Hong Liu;Liu Yongzhong;Deng Xiuxin. Obtaining of Ponkan (Citrus reticulata Blanco ) Tetraploid by Culturing Embryos of Mature Seeds[J]. ACTA HORTICULTURAE SINICA, 2005, 32(4): 688-690.

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Obtaining of Ponkan (Citrus reticulata Blanco ) Tetraploid by Culturing Embryos of Mature Seeds

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