Effects of Film Mulching on Improving Fruit Quality of Ponkan and Possible Mechanisms

doi:10.16420/j.issn.0513-353x.2021-0720

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2419-2430.doi: 10.16420/j.issn.0513-353x.2021-0720

• Research Notes • Previous Articles Next Articles

Effects of Film Mulching on Improving Fruit Quality of Ponkan and Possible Mechanisms

ZENG Yike¹, SHI Ying¹, CHEN Siyi², LI Guojing², HUANG Xianbiao², XIE Zongzhou¹, LI Chunlong¹, GUO Dayong¹^,^*(), LIU Jihong¹^,^*

¹Key Laboratory of Horticultural Plant Biology，College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China
²Technology Promotion Center of Dangyang Special Agricultural Industry，Dangyang，Hubei 444100，China

Received:2022-06-22 Revised:2022-07-13 Online:2022-11-25 Published:2022-11-25
Contact: GUO Dayong,LIU Jihong E-mail:liujihong@mail.hzau.edu.cn

Abstract

Abstract:

In the current study the effects of film mulching above the soil on sugar and acid content and peel color of Citrus reticulata‘Egan 1’ fruit and the possible molecular mechanisms were investigated. The results showed that film mulching could significantly increase the content of total soluble solids in the fruits，with the most prominent increase being detected in sucrose content. Film mulching promoted coloring of Ponkan fruits at an earlier stage，leading to more reddish peel at ripening. However，the contents of titratable acid and citric acid were not dramatically altered by film mulching. Six sucrose synthetase genes（CrSUS1-CrSUS6）and five sucrose phosphate synthetase genes（CrSPS1-CrSPS4a/b）were identified by analyzing the released genome of C. reticulata. Gene expression analysis showed that film mulching significantly up-regulated some of the CrSUS and CrSPS genes. In addition，film mulching increased activity of the SUS enzyme involved in sucrose synthesis，but decreased the one associated with sucrose decomposition. In conclusion，film mulching enhanced the synthesis of soluble sugars and increased the content of sugars by inducing the expression of SUS and SPS genes in Ponkan fruits. Meanwhile，film mulching effectively elevated the ratio of total soluble solids/titratable acid，leading to better flavor，and stimulated fruit coloring，which demonstrated that it has an obviously positive effect on improving fruit quality.

Key words: ponkan, Citrus reticulata, film mulching, fruit quality, total soluble solids, titratable acids, fruit color, sucrose metabolism

CLC Number:

S666.1

ZENG Yike, SHI Ying, CHEN Siyi, LI Guojing, HUANG Xianbiao, XIE Zongzhou, LI Chunlong, GUO Dayong, LIU Jihong. Effects of Film Mulching on Improving Fruit Quality of Ponkan and Possible Mechanisms[J]. Acta Horticulturae Sinica, 2022, 49(11): 2419-2430.

Figures/Tables 8

References 37

[1]	Albertini M V, Carcouet E, Pailly O, Gambotti C, Luro F, Berti L. 2006. Changes in organic acids and sugars during early stages of development of acidic and acidless citrus fruit. Journal of Agricultural & Food Chemistry, 5421:8335-8339.
[2]	Chen Fa-xing, Liu Xing-hui, Wu Li-song, Chen Li-song. 2010. Reflecting film mulch around tree trunks reduces acidity of loquat ripe fruits. Acta Horticulturae Sinica, 37 (11):1836-1842. (in Chinese)
	陈发兴, 刘星辉, 吴德宜, 陈立松. 2010. 树盘铺反光膜降低枇杷果实酸度. 园艺学报, 37 (11):1836-1842.
[3]	Gao Hai-wen, Jiang Ni, Liu Yong-zhong, Peng Shu-ang. 2013. Effects of film mulching on total soluble solution,titratable acid and contents of mineral elements in Ponkan(Citrus reticulata cv. Egan No. 1)fruit. Journal of Huazhong Agricultural University, 32 (5):45-49. (in Chinese)
	高海文, 姜妮, 刘永忠, 彭抒昂. 2013. 地表覆膜对椪柑果实糖酸品质及矿质元素的影响. 华中农业大学学报, 32 (5):45-49.
[4]	Glenn D M, Puterka G J. 2007. The use of plastic films and sprayable reflective particle films to increase light penetration in apple canopies and improve apple color and weight. HortScience, 42 (1):91-96. doi: 10.21273/HORTSCI.42.1.91 URL
[5]	Gong Rong-gao, Zhang Gang-lun. 2003. Research progress of sugar metabolism in citrus fruits. Journal of Sichuan Agricultural University, 21 (4):343-346. (in Chinese)
	龚荣高, 张光伦. 2003. 柑橘果实糖代谢的研究进展. 四川农业大学学报, 21 (4):343-346.
[6]	Guo Xiu-zhu, Song Yang, Liu Dong-feng, Lin Shao-sheng, Xu Xian-heng. 2020. Application effect of reflective film on grape cultivation. Journal of Zhejiang Agricultural Sciences, 61 (5):934-936. (in Chinese)
	郭秀珠, 宋洋, 刘冬峰, 林绍生, 徐贤亨. 2020. 反光膜在葡萄栽培上的应用效果. 浙江农业科学, 61 (5):934-936.
[7]	Huang Jun, Zhang Nu, Min Ze-ping. 2010. Effects of different ground mulching materials on fruit quality and soil temperature and water content of early-maturing Satsuma orange. South China Fruit Trees, 39 (3):15-17. (in Chinese)
	黄俊, 张弩, 闵泽萍. 2010. 不同地面覆盖材料对早熟温州蜜柑果实品质及桔园土壤温度和水分的影响效应研究. 中国南方果树, 39 (3):15-17.
[8]	Jiang Cui-cui, Fang Zhi-zhen, Pan Shao-lin, Zhou Dan-rong, Ye Xin-fu. 2019. Effect of reflective film coating on color and internal quality of plum fruit. South China Fruits, 48 (3):102-104,111. (in Chinese)
	姜翠翠, 方智振, 潘少霖, 周丹蓉, 叶新福. 2019. 地面覆反光膜对秋姬李果实色泽及内在品质的影响. 中国南方果树, 48 (3):102-104,111.
[9]	Jiang Ni, Gao Hai-wen,Jin Long-fei,Liu Yong-zhong,Peng Shu-ang. 2013. The impact of plastic film mulching on citrus fruit sugar accumulation and sucrose metabolism-related enzymes. Scientia Agricultura Sinica, 46 (2):317-324. (in Chinese)
	姜妮, 高海文, 金龙飞, 刘永忠, 彭抒昂. 2013. 地表覆膜对柑橘果实糖积累及蔗糖代谢酶活性的影响. 中国农业科学, 46 (2):317-324.
[10]	Jiang Qi-hang. 2019. Studies on the effects of bagging on carotenoid biogenesis and quality of pummelo fruits[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	姜启航. 2019. 套袋对柚果实类胡萝卜素代谢和品质的影响[硕士论文]. 武汉: 华中农业大学.
[11]	Ju Z Q, Duan Y S, Ju Z G. 1999. Effects of covering the orchard floor with reflecting films on pigment accumulation and fruit coloration in‘Fuji’apples. Scientia Horticulturae, 82 (1-2):47-56. doi: 10.1016/S0304-4238(99)00038-2 URL
[12]	Langenkämper G, Fung R W M, Newcomb R D, Atkinson R G, Gardner R C, Macrae E A. 2002. Sucrose phosphate synthase genes in plants belong to three different families. Journal of Molecular Evolution, 54 (3):322-332. pmid: 11847558
[13]	Li Fei-fei, Huang Cheng-neng, Xie Shen-xi, Lu Xiao-peng. 2018. Effects of excessive nitrogen application on orchard soil and tree mineral nutrients and fruit quality of ponkan. Journal of Southern Agriculture, 49 (4):748-756. (in Chinese)
	李菲菲, 黄成能, 谢深喜, 卢晓鹏. 2018. 施氮过量对椪柑园土壤和树体矿质养分及果实品质的影响. 南方农业学报, 49 (4):748-756.
[14]	Liang Hai-feng. 2018. The impact of plastic film mulch on the fruit quality,sugar and organic acid metabolism in citrus maxima‘Hongrou-miyou’[M. D. Dissertation]. Guangzhou: Guangzhou University. (in Chinese)
	梁海峰. 2018. 地表覆膜对红肉蜜柚果实品质及糖酸代谢影响的研究[硕士论文]. 广州: 广州大学.
[15]	Lowell C A, Tomlinson P T, Koch K E. 1989. Sucrose-metabolizing enzymes in transport tissue and adjacent sink structures in developing citrus fruit. Plant Physiology, 90:1394-1402. doi: 10.1104/pp.90.4.1394 pmid: 16666942
[16]	Lu Hai-fen. 2015. Effects of reflective film and mineral nutrition elements on fruit quality of Cara Cara Navel Orange[M. D. Dissertation]. Fuzhou: Fujian Agriculture and Forestry University. (in Chinese)
	卢海芬. 2015. 反光膜和矿质营养元素对红肉脐橙果实品质的影响[硕士论文]. 福州: 福建农林大学.
[17]	Luro F, Gatto J, Costantino G, Pailly O. 2011. Analysis of genetic diversity in Citrus. Plant Genetic Resources, 9 (2):218-211. doi: 10.1017/S1479262111000189 URL
[18]	Morimoto T, Ouchi Y, Shimizu M, Baloch M S. 2007. Dynamic optimization of watering Satsuma mandarin using neural networks and genetic algorithms. Agricultural Water Management, 44 (2):119-132. doi: 10.1016/S0378-3774(99)00088-8 URL
[19]	Pan Shao-lin, Lin Yan-juan, Fang Zhi-zhen, Zhou Dan-rong, Jiang Cui-cui, Ye Xin-fu. 2018. Effects of light on the accumulation of anthocyanin in plum of Plum. South China Fruits, 47 (4):126-128,131. (in Chinese)
	潘少霖, 林炎娟, 方智振, 周丹蓉, 姜翠翠, 叶新福. 2018. 光照对芙蓉李果肉花色苷积累的影响. 中国南方果树, 47 (4):126-128,131.
[20]	Shi Xue-gen, Chen Jun-wei, Xu Hong-xia, Liu Chun-rong, Zheng Jiang-cheng, Wu Hui, Xie Ming. 2011. Effects of vapor-permeable reflective film mulch on fruit quality of Ponkan tangerine. Journal of Fruit Science, 28 (3):418-422. (in Chinese)
	石学根, 陈俊伟, 徐红霞, 刘春荣, 郑江程, 吴慧, 谢鸣. 2011. 透湿性反光膜覆盖对椪柑果实品质的影响. 果树学报, 28 (3):418-422.
[21]	Tang Qi-zheng. 2020. Research of effects of fertilization, mulching and cross-pollination on fruit quality of Majia pummelo[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	唐启正. 2020. 施肥、覆膜和异花授粉处理对马家柚果实品质影响研究[硕士论文]. 武汉: 华中农业大学.
[22]	Violeta N, Ion T, Ionica M E. 2010. HPLC organic acid analysis in different citrus juices under reversed phase conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38 (1):44-48.
[23]	Wan Yong, Li Chun-ling, Zou Yuan-peng, Huang Xian-Biao, Peng Shu-ang, Deng Xiu-xin. 2011. Effects of different low-cost protected cultivations on quality of ponkan fruit. Journal of Huazhong Agricultural University, 30 (5):572-577. (in Chinese)
	万勇, 李春玲, 邹远鹏, 黄先彪, 彭抒昂, 邓秀新. 2011. 几种简易设施栽培对椪柑果实品质的影响. 华中农业大学学报, 30 (5):572-577.
[24]	Wang L, He F, Huang Y, He J X, Yang S Z, Zeng J W, Deng C L, Jiang X L, Fang Y W, Wen S H, Xu R W, Yu H W, Yang X M, Zhong G Y, Chen C W, Yan X, Zhou C F, Zhang H Y, Xie Z Z, Larkin R M, Deng X X, Xu Q. 2018. Genome of wild mandarin and domestication history of mandarin. Molecular Plant, 11 (8):1024-1037. doi: S1674-2052(18)30187-4 pmid: 29885473
[25]	Wang Li-fen, Wu Xian-feng, Zhu Jun-zhen. 2010. Studies on improving fruit quality of Satsuma orange by laying reflective film. South China Fruits, 39 (2):19-20. (in Chinese)
	王利芬, 吴险峰, 朱军贞. 2010. 铺设反光膜提高温州蜜柑果实品质的研究初报. 中国南方果树, 39 (2):19-20.
[26]	Wang Li-rong, Wu Jin-long. 2021. Review for the research of fruit tree germplasm and breeding of new varieties in the past seven decades in China. Acta Horticulturae Sinica, 48 (4):749-758. (in Chinese)
	王力荣, 吴金龙. 2021. 中国果树种质资源研究与新品种选育70年. 园艺学报, 48 (4):749-758.
[27]	Wang X Y, Wang P, Qi Y P, Zhou C P, Yang L T, Liao X Y, Wang L Q, Zhu D H, Chen L S. 2014. Effects of granulation on organic acid metabolism and its relation to mineral elements in Citrus grandis juice sacs. Food Chem, 145:984-990. doi: 10.1016/j.foodchem.2013.09.021 URL
[28]	Wei Qing-jiang, Ma Zhang-zheng, Le Si, Lei Chang-yu, Ma Qiao-li, Gu Qing-qing. 2020. Identification and expression analysis of sucrose-phosphate synthase (SPS) genes in citrus. Acta Horticulturae Sinica, 47 (2):334-344. (in Chinese)
	魏清江, 马张正, 勒思, 雷常玉, 马巧利, 辜青青. 2020. 柑橘磷酸蔗糖合酶基因CsSPS的鉴定和表达. 园艺学报, 47 (2):334-344.
[29]	Yakushiji H, Nonami H, Fukuyama T, Ono S, Hashimoto Y. 1996. Sugar accumulation enhanced by osmoregulation in satsuma mandarin fruit. Journal of the American Society for Horticultural Science, 121 (3):466-472. doi: 10.21273/JASHS.121.3.466 URL
[30]	Zeng Xiang-guo. 2005. Studies on contents and composition of sugar and acid in citrus from different species and production areas[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	曾祥国. 2005. 不同种类和产区柑橘糖酸含量及组成研究[硕士论文]. 武汉: 华中农业大学.
[31]	Zhang G F, Xie S X. 2014. Effects of plastic film mulching on quality and appearance of satsuma mandarin fruit. American Journal of Plant Sciences, 5 (26):3829-3835. doi: 10.4236/ajps.2014.526401 URL
[32]	Zhang Gui-fu, Xie Shen-xi. 2015. Effects of plastic mulching on fruit quality and appearance of satsuma orange. Acta Agriculturae Jiangxi, 27 (5):1-4. (in Chinese)
	张规富, 谢深喜. 2015. 地表覆膜对温州蜜柑果实品质和外观的影响. 江西农业学报, 27 (5):1-4.
[33]	Zhang L T. 2002. The sweetness of sugars. Journal of South China University of Technology(Natural Science Edition), 30 (1):89-91.
[34]	Zhang Shanglong, Yang Zongxin, Fang Jianping. 1988. The effect of polythenes mulch on the siaze and growth curves of sand pear(Pyrue Pyrifolia Nakai)fruits. Acta Horticulturae Sinica, 15 (1):1-6. (in Chinese)
	张上隆, 杨宗鑫, 方建平. 1988. 地膜覆盖对梨果实生长发育及品质的影响. 园艺学报, 15 (1):1-6.
[35]	Zhang Zhuo, Wang Lei, Gao Jie, Chen Qiu-ju, Wang Shi-ping, Zhang Cai-xi. 2019. Effect of moisture permeable reflective film mulching on crown illumination and fruit quality of protected sweet cherry. China Fruits,(3):54-56. (in Chinese)
	张卓, 王磊, 高洁, 陈秋菊, 王世平, 张才喜. 2019. 透湿性反光膜覆盖对设施甜樱桃树冠光照及果实品质的影响初报. 中国果树,(3):54-56.
[36]	Zhao Zhi-zhong, Zhang Shang-long, Xu Chang-jie, Chen Kun-song, Liu Shuan-tao. 2001. Roles of sucrose-metabolizing enzymes in accumulation of sugars in Satsuma mandarin fruit. Acta Horticulturae Sinica, 28 (2):112-118. (in Chinese)
	赵智中, 张上隆, 徐昌杰, 陈昆松, 刘拴桃. 2001. 蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用. 园艺学报, 28 (2):112-118.
[37]	Zhu Jian, Liu Ben-yong, Li Shu-ju, Bai Zhi-lan, Liu Cong, Deng Xiao-dong, Liu Ji-hong. 2020. Effects of 2,4-D on keeping citrus fruit fresh in tree and the underlying mechanisms. Acta Horticulturae Sinica, 47 (11):2086-2094. (in Chinese)
	祝建, 刘本勇, 李述举, 白芝兰, 刘聪, 邓晓东, 刘继红. 2020. 2,4-D在柑橘果实留树保鲜中的作用和可能机制研究. 园艺学报, 47 (11):2086-2094.

基因名称	上游引物（5′-3′）	下游引物（5′-3′）
Gene name	Forward primer	Reverse primer
CrSUS1	GAGTGGAGTGTATGGCTTCTG	CTGAACCTGCTTGCGATACA
CrSUS2	TAGCTGGGGTTTATGGCTTCTG	GACGGACTTTGCCAGATCAC
CrSUS3	TCTGATGGAGGGCTTAAAAGGA	GGTCTCACGCCTCTCAAGTT
CrSUS4	ATGTATCTAACCTTGATCGGCTTGA	TCCACAGCCAGAGGAACAGAT
CrSUS5	TTGAACAAGGGGCAGAAACT	TGACTGCTGGCTCTGACATT
CrSUS6	ACAAAGTGCTGAACATGGGA	TGCAGGTTGCTGGGATTCAA
CrSPS1	GGCTCGTCAGAAAAAGCAGC	GAGCCGATACATCGCTGACA
CrSPS2	CAGCTGGGGTTTCCTCCTTT	ACCAGAAACAGCGTCAGTCC
CrSPS3	TCAAGGTTGTTGCCACTCGT	CCTTGTTCCCGCTCCAATCT
CrSPS4a	CAGGAGCAGAGACCCGAAAA	ACCACATTTAACCTTGATCCTGC
CrSPS4b	ATATGTGCTGGCGCATTTGG	GCGGCATCATTACGACCTTG

基因名称	上游引物（5′-3′）	下游引物（5′-3′）
Gene name	Forward primer	Reverse primer
CrSUS1	GAGTGGAGTGTATGGCTTCTG	CTGAACCTGCTTGCGATACA
CrSUS2	TAGCTGGGGTTTATGGCTTCTG	GACGGACTTTGCCAGATCAC
CrSUS3	TCTGATGGAGGGCTTAAAAGGA	GGTCTCACGCCTCTCAAGTT
CrSUS4	ATGTATCTAACCTTGATCGGCTTGA	TCCACAGCCAGAGGAACAGAT
CrSUS5	TTGAACAAGGGGCAGAAACT	TGACTGCTGGCTCTGACATT
CrSUS6	ACAAAGTGCTGAACATGGGA	TGCAGGTTGCTGGGATTCAA
CrSPS1	GGCTCGTCAGAAAAAGCAGC	GAGCCGATACATCGCTGACA
CrSPS2	CAGCTGGGGTTTCCTCCTTT	ACCAGAAACAGCGTCAGTCC
CrSPS3	TCAAGGTTGTTGCCACTCGT	CCTTGTTCCCGCTCCAATCT
CrSPS4a	CAGGAGCAGAGACCCGAAAA	ACCACATTTAACCTTGATCCTGC
CrSPS4b	ATATGTGCTGGCGCATTTGG	GCGGCATCATTACGACCTTG

处理 Treatment	总甜度 Sweetness value	甜酸比 Sweetness/Acid	糖酸比 Sugar/Acid	固酸比 TSS/TA
对照Control	10 902.38 ± 184.27	1 975.43 ± 66.82	17.29 ± 0.62	21.02 ± 0.41
覆膜 Film mulching	12 056.88 ± 49.30^*	2 128.22 ± 18.89^*	18.61 ± 0.15^*	22.24 ± 0.31^*

处理 Treatment	总甜度 Sweetness value	甜酸比 Sweetness/Acid	糖酸比 Sugar/Acid	固酸比 TSS/TA
对照Control	10 902.38 ± 184.27	1 975.43 ± 66.82	17.29 ± 0.62	21.02 ± 0.41
覆膜 Film mulching	12 056.88 ± 49.30^*	2 128.22 ± 18.89^*	18.61 ± 0.15^*	22.24 ± 0.31^*

基因名称 Gene name	基因编号 Gene ID	蛋白长度/aa Protein length	分子量/kD Molecular weight	亚细胞定位 Subcellular localization	等电点 Isoelectric point	拟南芥Arabidopsis
基因名称 Gene name	基因编号 Gene ID	蛋白长度/aa Protein length	分子量/kD Molecular weight	亚细胞定位 Subcellular localization	等电点 Isoelectric point	同源基因 Homologous gene	氨基酸相似性/% Similarity of amino acid
CrSUS1	MSYJ123140	795	90.75	细胞膜Plasma membrane	5.77	AT5G20830	74
CrSUS2	MSYJ140250	808	92.62	细胞质 Cytoplasm	5.96	AT5G49190	82
CrSUS3	MSYJ114300	811	92.68	细胞质 Cytoplasm	6.04	AT4G02280	85
CrSUS4	MSYJ123080	805	92.17	细胞质 Cytoplasm	5.90	AT3G43190	85
CrSUS5	MSYJ034930	839	95.32	叶绿体Chloroplast	5.93	AT5G37180	74
CrSUS6	MSYJ073400	860	97.29	叶绿体Chloroplast	7.00	AT1G73370	73
CrSPS1	MSYJ121630	980	109.19	叶绿体Chloroplast	6.25	AT5G20280	78
CrSPS2	MSYJ156900	1 054	117.72	叶绿体Chloroplast	6.33	AT5G11110	72
CrSPS3	MSYJ073580	1 067	120.09	叶绿体Chloroplast	6.14	AT1G04920	78
CrSPS4a	MSYJ275740	848	94.59	叶绿体Chloroplast	6.27	AT4G10120	75
CrSPS4b	MSYJ214500	1 024	115.04	叶绿体Chloroplast	6.34	AT4G10120	72

Effects of Film Mulching on Improving Fruit Quality of Ponkan and Possible Mechanisms

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