Regeneration of Tetraploid Plants with Cecropin MB39 Gene from‘Royal Gala’Apple

ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (5): 392-398.

• 研究论文 • Previous Articles Next Articles

Regeneration of Tetraploid Plants with Cecropin MB₃₉ Gene from‘Royal Gala’Apple

Liu Qingzhong¹ , Zhao Hongjun¹ , Liu Peng¹ , Mong Rengong² , and Freddi A. Hammerschlag³

(¹ Shandong Institute of pomology , Tai’an , Shandong 271000;　² USDA/ ARS HCRL , Corvallis , OR 97330 , USA ;　³ USDA/ ARS , Fruit Laboratory , Beltsville , MD 20705 , USA)

Received:1900-01-01 Revised:1900-01-01 Online:2001-10-25 Published:2001-10-25

Abstract

Abstract: As part of the program to develop transgenic Malus ×Domestic. cv. ‘Royal Gala’tetraploid with improved traits , new germplasmof transgenic diploid and tetraploid plants with cecropin MB39 gene were regenerated. Seven transgenic diploid plants were obtained from etiolated internodal
explants by Agrobacterium tumefaciens-mediated transformation using the plasmid binary vector pGV containing a chimeric gene cecropin MB39 . The integration of the cecropin gene into apple genome was confirmed by PCR and Southern blotting analysis. It was proved that lines , TR-1 and TR-3 , had increased the resistance to fireblight disease caused by Erwinia amylovora. Twenty tetraploid lines were produced by cocultivating leaf explants from wild type and transgenic diploid shoots with colchicine in apple regeneration medium. Flow cytometry was used for ploidy etermination. The tetraploid plants were distinguishable from the diploid on morphological as well as cytogenetic grounds. Both the transgenic diploid and tetraploid plants are now being evaluated for growth development and disease resistance.

Key words: Cecropin MB₃₉, Transgenic, Apple, Tetraploid, Colchicine

CLC Number:

Liu Qingzhong;Zhao Hongjun;Liu Peng;Mong Rengong;and Freddi A. Hammerschlag. Regeneration of Tetraploid Plants with Cecropin MB₃₉ Gene from‘Royal Gala’Apple[J]. ACTA HORTICULTURAE SINICA, 2001, 28(5): 392-398.

[1]	ZHANG Ailing, TU Hongyan, XIAO Wang, ZHONG Xiaoqing, LU Qiuchan, CHENG Liping, LIN Xiaoping, and MAI Yuling. Morphology and Microstructure Characteristics of Diploid and Tetraploid Hedychium coronarium Cut Flowers [J]. Acta Horticulturae Sinica, 2023, 50(2): 345-358.
[2]	REN Fei, LU Miaomiao, LIU Jixiang, CHEN Xinli, LIU Daofeng, SUI Shunzhao, and MA Jing. Expression and Adversity Resistance Analysis of a Late Embryogenesis Abundant Protein Gene CpLEA from Chimonanthus praecox [J]. Acta Horticulturae Sinica, 2023, 50(2): 359-370.
[3]	YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, and YANG Hongqiang. Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin [J]. Acta Horticulturae Sinica, 2023, 50(2): 397-409.
[4]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YANG An, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Zhongping Youlei’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 1-2.
[5]	SUN Simiao, WANG Kun, GAO Yuan, WANG Dajiang, and LI Lianwen. A New Ornamental Crabapple Cultivar‘Zichen’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 267-268.
[6]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Pingyou 2’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 1-2.
[7]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Late Ripening Apple Cultivar‘Huayou Tianwa’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 3-4.
[8]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Mid-ripening Apple Cultivar‘Huayou Baomi’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 5-6.
[9]	YANG Ling, CONG Peihua, WANG Qiang, LI Wuxing, and KANG Liqun. A New Mid-ripening Apple Cultivar‘Huafeng’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 7-8.
[10]	LIU Chuanhe, HE Han, SHAO Xuehua, LAI Duo, KUANG Shizi, XIAO Weiqiang, LIU Yan. A New Pineapple Cultivar‘Yuetong’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 2053-2054.
[11]	GAO Yanlong, WU Yuxia, ZHANG Zhongxing, WANG Shuangcheng, ZHANG Rui, ZHANG De, WANG Yanxiu. Bioinformatics Analysis of Apple ELO Gene Family and Its Expression Analysis Under Low Temperature Stress [J]. Acta Horticulturae Sinica, 2022, 49(8): 1621-1636.
[12]	LIU Chaoyang, LIAO Zhichan, LU Xinxin, HE Yehua. Identification of CslD Gene Family in Pineapple and Functional Analysis of AcoCslD2a [J]. Acta Horticulturae Sinica, 2022, 49(8): 1650-1662.
[13]	ZHENG Xiaodong, XI Xiangli, LI Yuqi, SUN Zhijuan, MA Changqing, HAN Mingsan, LI Shaoxuan, TIAN Yike, WANG Caihong. Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress [J]. Acta Horticulturae Sinica, 2022, 49(7): 1401-1414.
[14]	XIA Yan, HUANG Song, WU Xueli, LIU Yiqi, WANG Miaomiao, SONG Chunhui, BAI Tuanhui, SONG Shangwei, PANG Hongguang, JIAO Jian, ZHENG Xianbo. Identification and Analysis of Apple Viruses Diseases Based on Virome Sequencing Technology [J]. Acta Horticulturae Sinica, 2022, 49(7): 1415-1428.
[15]	LIU Zhaoxia, ZHANG Xin, WANG Lu, MA Yuting, CHEN Qian, ZHU Zhanling, GE Shunfeng, JIANG Yuanmao. Effects of Fertilizer Hole Application Sites on Fine Root Growth,¹⁵N Absorption and Utilization,Yield and Quality of Apple Trees [J]. Acta Horticulturae Sinica, 2022, 49(7): 1545-1556.

Regeneration of Tetraploid Plants with Cecropin MB₃₉ Gene from‘Royal Gala’Apple

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments