Cloning and Expression Analysis of EuCML5 Gene in Eucommia ulmoides

doi:10.16420/j.issn.0513-353x.2019-0432

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (3): 590-600.doi: 10.16420/j.issn.0513-353x.2019-0432

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Cloning and Expression Analysis of EuCML5 Gene in Eucommia ulmoides

LIU Wei1,3,*，TENG Teng2,3,*，ZHAO Yichen2,3,**，and ZHAO Degang3,4

1College of Pharmacy，Guizhou University，Guiyang 550025，China；2College of Tea Science，Guizhou University， Guiyang 550025，China；3College of Life Sciences，Academy of Agricultural Bioengineering，The Key Laboratory of Plant Resources Conservation and Germplasm Innovationin Mountainous Region（Ministry of Education），Guizhou University，Guiyang 550025，China；4Guizhou Academy of Agricultural Sciences，Guiyang Branch Center for DUS Testing of New Plant Varieties of the Ministry of Agriculture of China，Guiyang 550006，China

Online:2020-03-25 Published:2020-03-25

Abstract

Abstract: Based on the Eucommia ulmoides genome database，the Calmodulin-like（CML）gene EuCML5 was cloned. The open reading frame of EuCML5 was 282 bp in length，encoding a total of 93 amino acids. It contained no transmembrane structure and signal peptide，belonging to intracellular protein. The amino acid sequence contained the EF-Hand motif，close to calmodulin-like protein of some woody plants in evolutionary relationship. Quantitative real-time PCR analysis revealed that the expression of EuCML5 was higher in Eucommia ulmoides roots and lower in stems and leaves. After treatment with drough（Mannitol），salt，methyl jasmonate and auxin，the expression of EuCML5 in Eucommia ulmoides was increased，indicating that EuCML5 can be induced by abiotic stress and phytohormone.In addition，calcium channel inhibitors LaCl3 and calmodulin antagonist TFP inhibited EuCML5expression.

Key words: Eucommia ulmoides, CML, expression, stress

CLC Number:

S 567

LIU Wei1,3,*，TENG Teng2,3,*，ZHAO Yichen2,3,**，and ZHAO Degang3,4. Cloning and Expression Analysis of EuCML5 Gene in Eucommia ulmoides[J]. ACTA HORTICULTURAE SINICA, 2020, 47(3): 590-600.

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Cloning and Expression Analysis of EuCML5 Gene in Eucommia ulmoides

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