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ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (4): 543-546.

• 蔬菜 • Previous Articles     Next Articles

Mapping Quantitative Trait Loci Influencing Cucumber Carpopodium Length Using Simple Sequence Repeat Markers

WANG Gui-ling, QIN Zhi-wei﹡, ZHOU Xiu-yan, and ZHAO Zhi-yun   

  1. (Department of Horticulture, Northeast Agricultural University, Harbin, 150030, China)
  • Received:2007-12-10 Revised:2008-03-07 Online:2008-04-25 Published:2008-04-25
  • Contact: QIN Zhi-wei

Abstract:

An F2 population from a cross between 129, a cucumber line with long carpopodium, and Z3, a cultivar with short carpopodium, was used to identify quantitative trait loci (QTLs) associated with cucumber carpopodium length. DNA was extracted from F2 plants and amplified using simple sequence repeat (SSR) marker. A total of five polymorphic SSR markers were used for QTL detection. Based on the data recorded, those five markers were used to construct the genetic linkage map using mapmaker 3.0b. QTLs were detected with Windows QTL Cartographer V2.0. Four markers CSWGATT01B, CSWGATT01C, CSCT335 and CSWGATT01A, from one linkage group, were significantly associated with carpopodium length. Qchl1 was linked with SSR maker CSWGATT01B and CSWGATT01C by 3.4 cM and 18.0 cM, respectively. It explained 18.49% of phenotypic variance.

Key words: Cucumis sativus, carpopodium length, SSR, QTL

CLC Number: