Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 482-492.doi: 10.16420/j.issn.0513-353x.2021-0244

• Research Papers • Previous Articles     Next Articles

Cloning and Functional Analysis of MdWRKY74 in Apple

XIANG Li1, ZHAO Lei1, WANG Mei1, LÜ Yi2, WANG Yanfang3, SHEN Xiang1, CHEN Xuesen1, YIN Chengmiao1,**(), MAO Zhiquan1,**()   

  1. 1State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
    2Weihai Acedemy of Agricultural Sciences,Weihai,Shandong 264200,China
    3College of Chemistry and Material Science,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Received:2021-04-26 Revised:2021-05-10 Online:2022-03-25 Published:2022-03-25
  • Contact: YIN Chengmiao,MAO Zhiquan;


In this study,a WRKY transcription factor gene,named MdWRKY74(XM_029090147.1)was obtained from apple rootstock M9T337(Malling 9 NAKBT337). The open reading frame of MdWRKY74 was 939 bp,which encoded 312 amino acids,and contained a typical WRKY domain. Amino acid sequence alignment and phylogenetic tree analysis showed that MdWRKY74 had the highest homology with Pyrus bretschneideri WRKY74. Subcellular localization assays showed that MdWRKY74 was located in the nucleus. Quantitative real-time PCR analysis showed that MdWRKY74 was expressed in different apple tissues. Overexpression of MdWRKY74 in Arabidopsis could improve the salt resistance of plants,and promote the expression of SOS1 and NHX1. The results showed that MdWRKY74 can be induced by salt stress,so it may participate in the regulation of salt resistance in apple.

Key words: apple, MdWRKY74, subcellular localization, salt stress, function analysis

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