QTL Mapping and Candidate Gene Identification Related to Ascorbic Acid Content in Tomato

doi:10.16420/j.issn.0513-353x.2023-0659

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (2): 219-228.doi: 10.16420/j.issn.0513-353x.2023-0659

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Next Articles

QTL Mapping and Candidate Gene Identification Related to Ascorbic Acid Content in Tomato

LIU Genzhong1,2，LI Fangman2，GE Pingfei2，TAO Jinbao2，ZHANG Xingyu2，YE Zhibiao2,3，and ZHANG Yuyang2,3,4,5,*

1College of Horticultural Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China；2National Key Laboratory of Germplasm Innovation and Utilization of Fruit and Vegetable Horticultural Crops，Huazhong Agricultural University，Wuhan 430070，China；3Hubei Hongshan Laboratory，Wuhan 430070，China；4Shenzhen Institute of Nutrition and Health，Huazhong Agricultural University，Wuhan 430070，China；5Shenzhen Branch of Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology，Shenzhen Institute of Agricultural Genomics，Chinese Academy of Agricultural Sciences，Shenzhen，Guangdong 518000，China

Online:2024-02-25 Published:2024-02-26

Abstract

Abstract: The F2 genetic segregating population was constructed via using high ascorbic acid（AsA）tomato accession TS-226 and low ascorbic acid tomato accession TS-228 as parents. The high-ascorbic acid and low-ascorbic acid pools were constructed respectively. Through bulked segregant analysis sequencing analysis（BSA-seq），the main QTL related to ascorbic acid was found，and the candidate gene related to ascorbic acid was located in the 58.00–60.15 Mb region of tomato chromosome 8. Furthermore，the ∆SNP-index data were analyzed to determine the main candidate gene SlPPO（Polyphenol oxidase）that controls the ascorbic acid content of tomato fruit. Using TS-228 as the background plant，transgenic lines with SlPPO overexpression and silencing were obtained by Agrobacterium-mediated transformation. Compared with the wild type，the total ascorbic acid content in the red ripe fruits of the overexpression lines OE-3 and OE-18 decreased by 18.89% and 26.56%，respectively. The total ascorbic acid content in the red ripe fruits of the silenced lines Ri-9 and Ri-16 increased by 37.53% and 63.93% relative to wild type，respectively. Taken together，SlPPO is the key gene to control the ascorbic acid content of tomato red ripe fruit，and plays a negative role in regulating fruit ascorbic acid content.

Key words: tomato, BSA-seq, ascorbic acid, QTL, SlPPO

CLC Number:

S 641.2

LIU Genzhong, LI Fangman, GE Pingfei, TAO Jinbao, ZHANG Xingyu, YE Zhibiao, and ZHANG Yuyang, . QTL Mapping and Candidate Gene Identification Related to Ascorbic Acid Content in Tomato[J]. Acta Horticulturae Sinica, 2024, 51(2): 219-228.

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QTL Mapping and Candidate Gene Identification Related to Ascorbic Acid Content in Tomato

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