Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (7): 1649-1664.doi: 10.16420/j.issn.0513-353x.2023-0449
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MENG Sida, HAN Leilei, XIANG Hengzuo, ZHU Meiyu, FENG Zhen, YE Yunzhu, SUN Meihua, LI Yanbing, ZHAO Liping, TAN Changhua, QI Mingfang*(), LI Tianlai*(
)
Received:
2024-04-25
Revised:
2024-06-03
Online:
2024-07-25
Published:
2024-07-19
Contact:
QI Mingfang, LI Tianlai
MENG Sida, HAN Leilei, XIANG Hengzuo, ZHU Meiyu, FENG Zhen, YE Yunzhu, SUN Meihua, LI Yanbing, ZHAO Liping, TAN Changhua, QI Mingfang, LI Tianlai. Research Progress on the Mechanism of Regulating the Number of Tomato Locules[J]. Acta Horticulturae Sinica, 2024, 51(7): 1649-1664.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0449
类型 Type | 位点/基因号 Locus/gene number | 功能 Function | 作用机制 Mechanism | 参考文献 Reference |
---|---|---|---|---|
QTL位点 Quantitative trait loci | lcn2.1/lc | 两个SNP突变导致心室数增加 Two SNP mutations lead to an increase in locule number | CArG元件突变导致阻遏功能丧失,WUS表达升高,分生组织变大 The mutation of CArG element leads to the loss of repression function,the increase of WUS expression and the enlargement of meristem | Muños et al., Rodriguez-Leal et al., |
fw11.1/fas | fas位点内部发生294 kb的倒置,导致心室数增加 An inversion of 294 kb occurred in fas locus,resulting in an increase in locule number | SlCLV3转录水平变化,影响WUS-CLV3途径 The change of SlCLV3 transcription level affects WUS-CLV3 pathway | Cong et al., | |
fab | FAB编码CLV3受体激酶 CLV1,突变导致心室数增加 FAB encodes CLV3 receptor kinase CLV1,and mutation leads to an increase in locule number | SlCLV3转录水平变化,影响WUS-CLV3途径 The change of SlCLV3 transcription level affects WUS-CLV3 pathway | Xu et al., | |
fin | FIN编码一个对CLV3进行翻译后修饰的阿拉伯糖基转移酶 FIN encodes an arabinosyltrans- ferase that performs post-translational modification on CLV3 | SlCLV3转录水平变化,影响WUS-CLV3途径 The change of SlCLV3 transcription level affects WUS-CLV3 pathway | Xu et al., | |
lcn3.1/eno | ENO位点突变使花分生组织变大,花器官数和果实心室数增加 ENO locus mutation makes the flower meristem bigger,and the number of flower organs and fruit locule increase | 抑制WUS转录,影响WUS-CLV3途径 Inhibition of WUS transcription and influence of WUS-CLV3 pathway | Fernandez-Lozano et al., | |
转录因子 Transcription factor | Solyc02g083950 | WUS沉默减少心室数 WUS silencing reduces the locule number | WUSCHEL是WUS-CLV3途径的核心成分,其表达量和表达区域直接影响分生组织分化 WUSCHEL is the core component of WUS-CLV3 pathway,and its expression level and expression region directly affect meristem differentiation | Li et al., |
Solyc03g117230 | ENO突变后,花分生组织增大,产生多心室果实 After the mutation of ENO,the flower meristem enlarged,resulting in multi-ventricular fruit | ENO可以直接结合WUSCHEL启动子上的GGC-box元件,从而直接调控WUSCHEL的表达区域和干细胞稳态 ENO can directly bind to GGC-box element on WUSCHEL promoter,thus directly regulating the expression region of WUS and stem cell homeostasis | Yuste-Lisbona et al., | |
Solyc10g076390 | BES1.8的超表达导致茎尖分生组织增大并产生较多的花器官和心室数 Over-expression of BES1.8 leads to the enlargement of shoot apical meristem and more floral organs and locule number | BES1.8可以直接与WUS结合并抑制WUS结合到SlCLV3和下游基因的启动子上,影响WUS-CLV3途径 BES1.8 can directly bind to WUS and inhibit WUSCHEL binding to the promoters of SlCLV3 and downstream genes,affecting the WUS-CLV3 pathway | Su et al., | |
Solyc01g100050 | SlTPL3的敲除影响番茄花和果实的发育,增加心室数 Knockout of SlTPL3 affected the development of tomato flowers and fruits,and increased locule number | SlTPL3和SlWUS的转录抑制模块负向调控SlPIN3以调节生长素的分布,同时正向调控SlGA2ox4以维持顶端分生组织中的低GA水平 Transcription inhibition modules of SlTPL3 and SlWUS negatively regulate SlPIN3 to regulate the distribution of auxin,while positively regulate SlGA2ox4 to maintain low GA level in apical meristem | Song et al., | |
Solyc02g087970 | ima突变体果实心室数增加 The ima mutant fruit locule number increased | 转录因子SlIMA可以通过与锌指蛋白家族转录因子SlKNU、转录共抑制子SlTPL1和组蛋白去乙酰化酶HAD19形成转录抑制复合物来抑制WUS的表达 Transcription factor SlIMA can inhibit the expression of WUS by forming a transcription inhibition complex with zinc finger protein family transcription factor SlKNU,transcription co-inhibitor SlTPL1 and histone deacetylase HAD19. | Bollier et al., | |
其他 Else | Solyc11g071380 | SlCLV3的敲除增加果实心室数 Knockout of SlCLV3 increased the fruit locule number | CLV3是一种小肽,与定位于质膜上的受体CLV1结合形成复合物启动下游级联反应抑制WUS表达 CLV3 is a small peptide,which combines with the receptor CLV1 located on the plasma membrane to form a complex to start the downstream cascade reaction and inhibit the expression of WUS | Schoof et al., |
Table 1 Main genetic factors regulating the locule number of tomato fruit
类型 Type | 位点/基因号 Locus/gene number | 功能 Function | 作用机制 Mechanism | 参考文献 Reference |
---|---|---|---|---|
QTL位点 Quantitative trait loci | lcn2.1/lc | 两个SNP突变导致心室数增加 Two SNP mutations lead to an increase in locule number | CArG元件突变导致阻遏功能丧失,WUS表达升高,分生组织变大 The mutation of CArG element leads to the loss of repression function,the increase of WUS expression and the enlargement of meristem | Muños et al., Rodriguez-Leal et al., |
fw11.1/fas | fas位点内部发生294 kb的倒置,导致心室数增加 An inversion of 294 kb occurred in fas locus,resulting in an increase in locule number | SlCLV3转录水平变化,影响WUS-CLV3途径 The change of SlCLV3 transcription level affects WUS-CLV3 pathway | Cong et al., | |
fab | FAB编码CLV3受体激酶 CLV1,突变导致心室数增加 FAB encodes CLV3 receptor kinase CLV1,and mutation leads to an increase in locule number | SlCLV3转录水平变化,影响WUS-CLV3途径 The change of SlCLV3 transcription level affects WUS-CLV3 pathway | Xu et al., | |
fin | FIN编码一个对CLV3进行翻译后修饰的阿拉伯糖基转移酶 FIN encodes an arabinosyltrans- ferase that performs post-translational modification on CLV3 | SlCLV3转录水平变化,影响WUS-CLV3途径 The change of SlCLV3 transcription level affects WUS-CLV3 pathway | Xu et al., | |
lcn3.1/eno | ENO位点突变使花分生组织变大,花器官数和果实心室数增加 ENO locus mutation makes the flower meristem bigger,and the number of flower organs and fruit locule increase | 抑制WUS转录,影响WUS-CLV3途径 Inhibition of WUS transcription and influence of WUS-CLV3 pathway | Fernandez-Lozano et al., | |
转录因子 Transcription factor | Solyc02g083950 | WUS沉默减少心室数 WUS silencing reduces the locule number | WUSCHEL是WUS-CLV3途径的核心成分,其表达量和表达区域直接影响分生组织分化 WUSCHEL is the core component of WUS-CLV3 pathway,and its expression level and expression region directly affect meristem differentiation | Li et al., |
Solyc03g117230 | ENO突变后,花分生组织增大,产生多心室果实 After the mutation of ENO,the flower meristem enlarged,resulting in multi-ventricular fruit | ENO可以直接结合WUSCHEL启动子上的GGC-box元件,从而直接调控WUSCHEL的表达区域和干细胞稳态 ENO can directly bind to GGC-box element on WUSCHEL promoter,thus directly regulating the expression region of WUS and stem cell homeostasis | Yuste-Lisbona et al., | |
Solyc10g076390 | BES1.8的超表达导致茎尖分生组织增大并产生较多的花器官和心室数 Over-expression of BES1.8 leads to the enlargement of shoot apical meristem and more floral organs and locule number | BES1.8可以直接与WUS结合并抑制WUS结合到SlCLV3和下游基因的启动子上,影响WUS-CLV3途径 BES1.8 can directly bind to WUS and inhibit WUSCHEL binding to the promoters of SlCLV3 and downstream genes,affecting the WUS-CLV3 pathway | Su et al., | |
Solyc01g100050 | SlTPL3的敲除影响番茄花和果实的发育,增加心室数 Knockout of SlTPL3 affected the development of tomato flowers and fruits,and increased locule number | SlTPL3和SlWUS的转录抑制模块负向调控SlPIN3以调节生长素的分布,同时正向调控SlGA2ox4以维持顶端分生组织中的低GA水平 Transcription inhibition modules of SlTPL3 and SlWUS negatively regulate SlPIN3 to regulate the distribution of auxin,while positively regulate SlGA2ox4 to maintain low GA level in apical meristem | Song et al., | |
Solyc02g087970 | ima突变体果实心室数增加 The ima mutant fruit locule number increased | 转录因子SlIMA可以通过与锌指蛋白家族转录因子SlKNU、转录共抑制子SlTPL1和组蛋白去乙酰化酶HAD19形成转录抑制复合物来抑制WUS的表达 Transcription factor SlIMA can inhibit the expression of WUS by forming a transcription inhibition complex with zinc finger protein family transcription factor SlKNU,transcription co-inhibitor SlTPL1 and histone deacetylase HAD19. | Bollier et al., | |
其他 Else | Solyc11g071380 | SlCLV3的敲除增加果实心室数 Knockout of SlCLV3 increased the fruit locule number | CLV3是一种小肽,与定位于质膜上的受体CLV1结合形成复合物启动下游级联反应抑制WUS表达 CLV3 is a small peptide,which combines with the receptor CLV1 located on the plasma membrane to form a complex to start the downstream cascade reaction and inhibit the expression of WUS | Schoof et al., |
Fig. 2 QTL locus control fruit locules of tomato ↓:Chromosome inversion in two↓;Red box:Gene coding region;×:SNP site;_:nucleotide at SNP site;^:amino acid change; *:Termination codon;↑:InDel site. Fascinated:294 kb inversion between SlCLV3 promoter and the first intron of SlYABBY2b;lc:2 SNPs in the downstream of SlWUS coding region influence SlWUS expression;fab:Alanine at positon 841 is replaced by valine in SlCLV1 kinase domain;fin:Proline at position 293 is replaced by serine or mutate the coding lysine codon at position 358 to a termination codon in SlHPAT3 conding region;eno:85 bp InDel at 107 bp upstream of start codon affect SlENO expression.
Fig. 3 Genes particate WUS-CLV3 pathway control fruit locule number of tomato Black arrows or black linkers indicate promotive or negative effects,respectively,at the transcriptional level or in the signal transduction pathway. SlKNU:Zinc finger protein family transcription factor;SlTPL1:Transcription co-suppressor;HAD19:Histone deacetylase.
Fig. 5 Regulation of WUS by ROS in apical meristen Black(blue)arrows or the black(blue)linkers indicates a catalytic or inhibitory effect in the pathway,respectively,with the blue arrow highlighting the primary function of $\mathrm{O}_{\dot{2}}^{-}$ in the fate determination of plant stem cells. CZ:Central zone;PZ:peripheral zone;$\mathrm{O}_{\dot{2}}^{-}$:superoxide anion.
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