Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (2): 219-228.doi: 10.16420/j.issn.0513-353x.2023-0659
• Genetic&Breeding?Germplasm Resources?Molecular Biology • Next Articles
LIU Genzhong1,2, LI Fangman2, GE Pingfei2, TAO Jinbao2, ZHANG Xingyu2, YE Zhibiao2,3, ZHANG Yuyang2,3,4,5,*()
Received:
2023-09-03
Revised:
2024-01-15
Online:
2024-02-25
Published:
2024-02-26
Contact:
ZHANG Yuyang
LIU Genzhong, LI Fangman, GE Pingfei, TAO Jinbao, ZHANG Xingyu, YE Zhibiao, ZHANG Yuyang. QTL Mapping and Candidate Gene Identification Related to Ascorbic Acid Content in Tomato[J]. Acta Horticulturae Sinica, 2024, 51(2): 219-228.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0659
目的 Purpose | 正向引物(5′-3′) Forward primer | 反向的引物(5′-3′) Reverse primer |
---|---|---|
SlPPO for qRT-PCR | TGCCAACCAAAACAATGGTGA | CAGCATAGGCTAAGGGTGCT |
SlPPO for Ri | GGGGACAAGTTTGTACAAAAAAGCA GGCTGAGTCGGAGACTGCTTGGATACA | GGGGACCACTTTGTACAAGAAAGCT GGGTGCATTCACATTGTCATCCTCGTT |
SlPPO for OE | AAAAAGCAGGCTATGTCTTCTTCTT TTTGGAGTAATTC | AGAAAGCTGGGTTCAACAGTCCTCC AAAGATTCTGTA |
Table 1 Primers for the genes related to the tomato ascorbic acid
目的 Purpose | 正向引物(5′-3′) Forward primer | 反向的引物(5′-3′) Reverse primer |
---|---|---|
SlPPO for qRT-PCR | TGCCAACCAAAACAATGGTGA | CAGCATAGGCTAAGGGTGCT |
SlPPO for Ri | GGGGACAAGTTTGTACAAAAAAGCA GGCTGAGTCGGAGACTGCTTGGATACA | GGGGACCACTTTGTACAAGAAAGCT GGGTGCATTCACATTGTCATCCTCGTT |
SlPPO for OE | AAAAAGCAGGCTATGTCTTCTTCTT TTTGGAGTAATTC | AGAAAGCTGGGTTCAACAGTCCTCC AAAGATTCTGTA |
Fig. 1 Total ascorbic acid content of red ripe tomato fruit in parents, F1 and F2 population Different lowercase letters indicate significant differences(P < 0.05).
样品名称 Sample | Q20比率/% Q20 rate | 高质量读长数/条 Clean reads | 高质量碱基数/bp Clean bases | 读长数比对率/% Mapping rate of reads | 基因组测序深度/× Sequencing depth |
---|---|---|---|---|---|
低AsA池 Low AsA pool | 93.62 | 143 821 112 | 21 573 166 800 | 83.95 | 26.18 |
高AsA池 High AsA pool | 93.67 | 144 629 966 | 21 694 494 900 | 82.94 | 26.33 |
Table 2 Summary of whole-genome re-sequencing data
样品名称 Sample | Q20比率/% Q20 rate | 高质量读长数/条 Clean reads | 高质量碱基数/bp Clean bases | 读长数比对率/% Mapping rate of reads | 基因组测序深度/× Sequencing depth |
---|---|---|---|---|---|
低AsA池 Low AsA pool | 93.62 | 143 821 112 | 21 573 166 800 | 83.95 | 26.18 |
高AsA池 High AsA pool | 93.67 | 144 629 966 | 21 694 494 900 | 82.94 | 26.33 |
多酚氧化酶基因 Polyphenol oxidase gene | SNP位置 SNP location | 参考碱基 Reference base | 低AsA池Low AsA pool | 高AsA池High AsA pool | △SNP-index | ||
---|---|---|---|---|---|---|---|
Base | SNP-index | Base | SNP-index | ||||
Solyc08g074620 | 58745745 | C | C | 0 | Y | 0.792 | 0.792 |
58746330 | C | Y | 0.208 | Y | 0.833 | 0.625 | |
Solyc08g074630 | 58756071 | G | S | 0.312 | S | 0.778 | 0.466 |
58757391 | A | W | 0.346 | W | 0.762 | 0.416 | |
Solyc08g074640 | 58771375 | G | G | 0 | R | 0.750 | 0.750 |
58772995 | T | T | 0 | Y | 0.769 | 0.769 | |
Solyc08g074650 | 58785356 | A | A | 0 | G | 0.938 | 0.938 |
58785423 | C | C | 0 | T | 0.905 | 0.905 | |
Solyc08g074680 | 58830789 | G | S | 0.296 | C | 0.929 | 0.633 |
58841121 | T | Y | 0.292 | Y | 0.857 | 0.565 | |
Solyc08g074690 | 58851893 | A | W | 0.250 | W | 0.714 | 0.464 |
58852031 | G | G | 0 | R | 0.696 | 0.696 |
Table 3 Variation analysis of candidate genes in the target region of tomato chromosome 8
多酚氧化酶基因 Polyphenol oxidase gene | SNP位置 SNP location | 参考碱基 Reference base | 低AsA池Low AsA pool | 高AsA池High AsA pool | △SNP-index | ||
---|---|---|---|---|---|---|---|
Base | SNP-index | Base | SNP-index | ||||
Solyc08g074620 | 58745745 | C | C | 0 | Y | 0.792 | 0.792 |
58746330 | C | Y | 0.208 | Y | 0.833 | 0.625 | |
Solyc08g074630 | 58756071 | G | S | 0.312 | S | 0.778 | 0.466 |
58757391 | A | W | 0.346 | W | 0.762 | 0.416 | |
Solyc08g074640 | 58771375 | G | G | 0 | R | 0.750 | 0.750 |
58772995 | T | T | 0 | Y | 0.769 | 0.769 | |
Solyc08g074650 | 58785356 | A | A | 0 | G | 0.938 | 0.938 |
58785423 | C | C | 0 | T | 0.905 | 0.905 | |
Solyc08g074680 | 58830789 | G | S | 0.296 | C | 0.929 | 0.633 |
58841121 | T | Y | 0.292 | Y | 0.857 | 0.565 | |
Solyc08g074690 | 58851893 | A | W | 0.250 | W | 0.714 | 0.464 |
58852031 | G | G | 0 | R | 0.696 | 0.696 |
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