Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (5): 982-992.doi: 10.16420/j.issn.0513-353x.2023-0060
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
LIU Zeying, SUN Shuai, LIU Zhiqiang, CUI Xia*(), LI Ren*(
)
Received:
2023-12-07
Revised:
2024-03-26
Online:
2024-05-25
Published:
2024-05-29
LIU Zeying, SUN Shuai, LIU Zhiqiang, CUI Xia, LI Ren. Mapping of the Sharp Blossom-end Gene and Screening of Candidate Gene in Tomato[J]. Acta Horticulturae Sinica, 2024, 51(5): 982-992.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0060
Fig. 1 Phenotype analysis of the cultivated tomato MM and its mutant shb fruits Data analysis was performed by student’s t-test. *,** means statistically significant differences(* P < 0.05;** P < 0.01). H:Maximum height;W:Maximum width.
Fig. 2 The morphological observation of pericarp cells in the cultivated tomato MM and the mutant shb fruits The black line a indicates the cell layer number,the red rectangle indicates the total cell number,and the orange lines b and c indicate the cell length and width,respectively. Data analysis was performed by student’s t-test. ** means statistically extremely significant differences(P < 0.01).
Fig. 3 The morphology of the blossom end in the cultivated tomato MM and the mutant shb at 0 and 6 days post-anthesis The red arrow indicates the location where the style would drop.
Fig. 4 The cell morphological observation of the style base in the cultivated tomato MM and the shb mutant fruits at 0,3,6 days post-anthesis The red arrow indicates the location where the style would drop.
Fig. 6 Δ(SNP-index) from BSA-seq for mapping the genomic regions controlling tomato sharp blossom end The scattered points in the figure represent the calculated values of Δ(SNP-index). The orange straight line represents the |Δ(SNP-index)| > 0.8. The red arrows indicate the five screened SNP/InDels located near genes,two of which have equal delta SNP-index values.
单核苷酸多态性 SNP | 突变在基因上位置 Location of mutation | 候选基因 Candidate gene | 基因注释 Annotation |
---|---|---|---|
SL4.0ch06_ 38607063 | 启动子 Promoter | Solyc06g065570 | N-α-乙酰转移酶50 N-α-Acetyltransferase 50 |
SL4.0ch06_ 38607063 | 3′端非编码区 3′UTR | Solyc06g065580 | 尿苷-胞苷激酶 Uridine kinase |
SL4.0ch08_ 55974557 | 启动子 Promoter | Solyc08g068740 | 未知蛋白 Unknown protein |
SL4.0ch08_ 55974560 | 启动子 Promoter | Solyc08g068740 | 未知蛋白 Unknown protein |
SL4.0ch09_ 1411527 | 启动子 Promoter | Solyc09g007860 | 钙依赖的磷脂结合蛋白Calcium-dependent lipid-binding |
Table 1 SNP and candidate genes list associated with the sharp bloosom end in tomato
单核苷酸多态性 SNP | 突变在基因上位置 Location of mutation | 候选基因 Candidate gene | 基因注释 Annotation |
---|---|---|---|
SL4.0ch06_ 38607063 | 启动子 Promoter | Solyc06g065570 | N-α-乙酰转移酶50 N-α-Acetyltransferase 50 |
SL4.0ch06_ 38607063 | 3′端非编码区 3′UTR | Solyc06g065580 | 尿苷-胞苷激酶 Uridine kinase |
SL4.0ch08_ 55974557 | 启动子 Promoter | Solyc08g068740 | 未知蛋白 Unknown protein |
SL4.0ch08_ 55974560 | 启动子 Promoter | Solyc08g068740 | 未知蛋白 Unknown protein |
SL4.0ch09_ 1411527 | 启动子 Promoter | Solyc09g007860 | 钙依赖的磷脂结合蛋白Calcium-dependent lipid-binding |
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