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Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (5): 982-992.doi: 10.16420/j.issn.0513-353x.2023-0060

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles     Next Articles

Mapping of the Sharp Blossom-end Gene and Screening of Candidate Gene in Tomato

LIU Zeying, SUN Shuai, LIU Zhiqiang, CUI Xia*(), LI Ren*()   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2023-12-07 Revised:2024-03-26 Online:2024-05-25 Published:2024-05-29

Abstract:

The sharp blossom-end mutant was obtained from cultivar tomato Money Maker(MM),named as shb. Fruits of shb mutants had a pointed tip and a predominantly increased fruit shape index,shown heart-shaped with smaller size,suggesting that SHB regulates tomato fruit size and shape. The freehand section and paraffin section observations showed that the pericarp thickness and cell layers of shb mutants were significantly decreased compared with MM. During early style development,different from the small and dense cells at the base of the MM style,the shb mutant style base and the cells at this region continued to expand,eventually forming a pointed end after style dropping. An F2 population which was derived from a cross between MM(flat blossom end)and shb(sharp blossom end)was generated. Genetic analysis indicated the sharp blossom end was a qualitative trait controlled by a single recessive gene. The individual plants with extreme fruit phenotypes of the pointed end and the flat one were divided into two groups for DNA pooling,and four candidate genes were preliminarily screened by using Bulked Segregant Analysis Coupled with Whole-Genome Sequencing(BSA-Seq).

Key words: tomato, blossom-end, fruit shape, cell division