Abstract: Simple sequence repeat（SSR）loci were searched and analyzed in the leaf transcriptomic sequence of Vaccinium corymbosum‘Brigitta’. The frequency of SSR was 21.32%，accounting for 22 058 SSR loci. The highest type of SSR motif was dinucleotides with the frequency of 77.70%，followed by trinucleotides with the frequency of 21.45%. Fifty-three random pairs of primers were used to perform polymerase chain reaction（PCR）in 8 accessions in Vaccinium genus. A total of 15 pairs of core primers with clear and stable amplification bands were screened from above primers. Genetic diversity of 39 accessions in genus of Vaccinium was estimated using the 15 core primers. The results showed that the maximum number of effective alleles was 7.11（VcSSR19）and the minimum value was 1.41（VcSSR41），with an average value of 3.90. The average of Shannon diversity index was 1.55，ranging from 0.64 to 2.31. The range of observed heterozygosity and expected heterozygosity were 0.050–0.900 and 0.293–0.870，with mean values of 0.457 and 0.677，respectively. Five polymorphic loci could be used to distinguish 38 accessions. Southern highbush blueberry cultivars‘Legacy’and‘Bluerainare’were more closely related to cranberry cultivars and wild relatives of Vaccinium，which would be suitable cultivars for cross breeding with wild species in Vaccinium.

Key words: blueberry, transcriptome, SSR, phylogenetic relationship, polymorphism