Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1081-1091.doi: 10.16420/j.issn.0513-353x.2021-0355

• Research Papers • Previous Articles     Next Articles

Identification of Pigment Components and Key Genes in Carotenoid Pathway in Mutants of Chimeric Ananas comosus var. bracteatus

ZHOU Xuzixin, YANG Wei, MAO Meiqin, XUE Yanbin, MA Jun()   

  1. College of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2021-06-15 Revised:2021-09-07 Online:2022-05-25 Published:2022-05-25
  • Contact: MA Jun


In order to explore how the phenotype of yellow mutants of chimeric Ananas comosus var. bracteatus formed,chimeral leaves in red stage and yellow mutants were taken in this study for comprehensive and comparative analysis using CIEL*a*b* coloration,pigment determination,histocytological observation,LC-MS/MS detection and illumina RNA-seq. Results of CIEL*a*b* indicated that the color of the green central part and red margin part of the chimeric leaves was consisted of green-yellow and red-yellow color,respectively. The yellow mutant showed bright yellow color with extremely slight green. The significant decrease of total chlorophyll content,Chl.a/b ratio,total anthocyanin content and the significant increase of the proportion of carotenoid and Chl.b of the yellow mutant leaf resulted in the loss of green and red color and turned to yellow. The yellow pigments were scattered in a bit of mesophyll cells of the yellow mutant leaf while the other leaf cells were transparent and colorless. Lutein accounted for 79% of the 12 detected carotenoids content which is the dominant pigment of the yellow mutant leaf. The decrease of PSY1 expression and phytoene,carotenoid,lutein content indicated that PSY1 probably was a key gene responsible for the carotenoid biosynthesis limitation in the yellow mutant of chimeric Ananas comosus var. bracteatus.

Key words: Ananas comosus var. bracteatus, yellow mutant, carotenoid, metabolome, transcriptome

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